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pBluescript II KS(+) EACMCV DNA-A 1.6mer

Plasmid

#159136

Purpose

Infectious clone containing a partial-tandem-dimer of East African cassava mosaic Cameroon virus 1998 DNA-A

Depositor

Vincent Fondong

Article

Hoyer et al Microbiol Resour Announc. 2020 Nov 12;9(

Insert

EACMCV DNA-A 1.6mer

Use

Infectious clone for bombarding plants

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pESC-URA-GFP-Ubc9ts

Plasmid

#20369

Depositor

Judith Frydman

Article

Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

Insert

SUMO-conjugating enzyme Ubc9 (UBC9 Budding Yeast)

Use

Yeast/bacteria shuttle

Tags

GFP

Expression

Yeast

Mutation

Y68L This mutation renders Ubc9 temperature-sens…

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only
pESC-LEU-GFP-VHL

Plasmid

#21053

Depositor

Judith Frydman

Article

Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

Insert

von Hippel-Lindau tumor suppressor (VHL Human)

Use

Yeast/bacteria shuttle

Tags

GFP

Expression

Yeast

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only
His6-TEV-mCerulean3-pBluescript

Plasmid

#166757

Purpose

For purification of mCerulean3 fluorophore

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

mCerulean3

Tags

6x Histidine tag, and TEV protease site

Expression

Bacterial

Promoter

T7

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pEF-pIgH-ESCargo(FTV)

Plasmid

#140160

Purpose

Expresses a regulatable secretory cargo for mammalian cells

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

piGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V)

Tags

ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

Expression

Mammalian

Promoter

EF-1a

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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His6-TEV-Venus-pBluescript

Plasmid

#166756

Purpose

For purification of Venus fluorophore

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

Venus

Tags

6x Histidine tag, and TEV protease site

Expression

Bacterial

Promoter

T7

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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pBluescript-IC3-FLAG-Tev-ZZ

Plasmid

#170311

Purpose

Plasmid for endogenous C-terminal tagging of the Tetrahymena thermophila Outer Dynein Arm intermediate chain 3 with FLAG and ZZ tags.

Depositor

Andrew Carter

Article

Mali et al Science. 2021 Feb 26;371(6532):910-916.

Insert

Outer Dynein Arm Intermediate Chain 3

Tags

FLAG, ZZ

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pESC-URA::CYP71AV1/CPR

Plasmid

#20118

Depositor

Jay Keasling

Article

Ro et al Nature. 2006 Apr 13. 440(7086):940-3.

Insert

amorphadiene oxidase and p450 reductase

Use

Synthetic Biology

Expression

Yeast

Available Since

March 2, 2009

Availability

Academic Institutions and Nonprofits only
pESC-URA-GFP-Ubc9wt

Plasmid

#20368

Depositor

Judith Frydman

Article

Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

Insert

SUMO-conjugating enzyme Ubc9 (UBC9 Budding Yeast)

Use

Yeast/bacteria shuttle

Tags

GFP

Expression

Yeast

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only
pBluescript II KS(+) ACMV DNA-A 1.4mer

Plasmid

#159134

Purpose

Infectious clone containing a partial-tandem-dimer of African cassava mosaic virus CM:2:98 DNA-A

Depositor

Vincent Fondong

Article

Hoyer et al Microbiol Resour Announc. 2020 Nov 12;9(

Insert

ACMV DNA-A 1.4mer

Use

Infectious clone for bombarding plants

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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plko-shmfn2-mfn2-Rescue

Plasmid

#160655

Purpose

"Rescue mfn2 expression by adding the guide-resistant-mutant mfn2 sequence"

Depositor

Qing Deng

Article

Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

Insert

mfn2 (MFN2 Human)

Use

Lentiviral

Promoter

CMV

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pTAJAK-71 (pESC-NatMXsyn-USER)

Plasmid

#78232

Purpose

Empty vector, for the insertion of gRNA expression cassettes into the vector.

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5 HA-GST-Presc-mAkt1 (wt)

Plasmid

#48805

Purpose

expresses wt Akt1

Depositor

David Sabatini

Article

Kang et al Science. 2013 Jul 26;341(6144):1236566.

Insert

Akt1 (Akt1 Mouse)

Tags

HA-GST-Presc

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2013

Availability

Academic Institutions and Nonprofits only
pESC-URA-Rnq1-GFP

Plasmid

#21051

Depositor

Judith Frydman

Article

Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

Insert

Rnq1 (RNQ1 Budding Yeast)

Use

Yeast/bacteria shuttle

Tags

GFP

Expression

Yeast

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only
Pet15b-Endosomal Escape Control

Plasmid

#186552

Purpose

Negative Control confirming the need for a CPP peptide in mediating internalization

Depositor

Jean Gariepy

Article

Mohammed et al J Control Release. 2012 Nov 28;164(1):58

Insert

6H-ETA-GFP

Tags

6xHis and EGFP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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Pet15b-Internalization and Endosomal Escape

Plasmid

#186551

Purpose

Internalization and Endosomal Escape to effectively route a protein cargo to the cytosol of cells

Depositor

Jean Gariepy

Article

Mohammed et al J Control Release. 2012 Nov 28;164(1):58

Insert

10R-8His-ETA-EGFP

Tags

8xHis and EGFP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-PICK1-rescue

Plasmid

#72573

Purpose

Expresses myc-PICK1 shRNA resistant cDNA

Depositor

Victor Anggono, Richard Huganir

Article

Anggono et al J Neurosci. 2011 Feb 9;31(6):2188-96. do

Insert

PICK1 (Pick1 Rat)

Tags

Myc

Expression

Mammalian

Mutation

5 silent mutations around shRNA recognition seque…

Promoter

CMV

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pESC-Leu-SopB(C460S)

Plasmid

#183671

Purpose

Inducible Salmonella Typhimurium SopB (catalytically inactive) for expression in yeast

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Salmonella enterica serovar Typhimurium)

Expression

Yeast

Mutation

C460S

Promoter

GAL1

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pESC-NAT-Ago-Dcr

Plasmid

#160381

Purpose

Overexpression of Ago-Dcr fusion protein in yeast

Depositor

John McCusker

Insert

Ago- Dcr

Expression

Yeast

Promoter

GAL

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBlueScript II SK-SNAP donor for H3f3b

Plasmid

#186933

Purpose

Genomic targeting of SNAP tag at H3f3b C-terminal

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

SNAP donor for H3f3b (H3f3b Mouse)

Tags

SNAP tag

Expression

Mammalian

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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Flag-TRIM24-C840W shRescue

Plasmid

#28137

Depositor

Michelle Barton

Article

Tsai et al Nature. 2010 Dec 16. 468(7326):927-32.

Insert

TRIM24 (TRIM24 Human)

Tags

FLAGX2

Expression

Mammalian

Mutation

C840Wmutated at aa 308 and 309 for shRNA rescue; …

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
PflQ2MT Pseudomonas fluorescens Q2-87 Metallothionein

Plasmid

#200297

Purpose

Bacterial expression plasmids for production of recombinant Pseudomonas fluorescens Q2-87 Metallothionein

Depositor

Artur Krężel

Article

Mosna et al Metallomics. 2023 Oct 4;15(10):mfad061.

Insert

Gene of metallothionein with Chiting Binding Domanin-Intein

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pBlueScript II SK-SNAP donor for Hist1h3g

Plasmid

#186932

Purpose

Genomic targeting of SNAP tag at Hist1h3g C-terminal

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

SNAP donor for Hist1h3g (H3c8 Mouse)

Tags

SNAP

Expression

Mammalian

Promoter

T7

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pESC-LEU-IpgD(C439S)

Plasmid

#183673

Purpose

Inducible Shigella flexneri IpgD (catalytically inactive) for expression in yeast

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

IpgD

Expression

Yeast

Mutation

C439S

Promoter

GAL1

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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YIplac204-pOst1-ESCargo(Orange)

Plasmid

#140157

Purpose

Expresses a regulatable secretory cargo for yeast at the TRP1 locus

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pOst1-APVNTT-E2-Orange-FKBP(LV)(C22V)

Expression

Yeast

Promoter

TPI1

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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YIplac128-pOst1-ESCargo(Orange)

Plasmid

#140158

Purpose

Expresses a regulatable secretory cargo for yeast at the LEU2 locus

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pOst1-APVNTT-E2-Orange-FKBP(LV)(C22V)

Expression

Yeast

Promoter

TPI1

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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YIplac128-pOst1-ESCargo(Crimson)

Plasmid

#140156

Purpose

Expresses a regulatable secretory cargo for yeast at the LEU2 locus

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)

Expression

Yeast

Promoter

TPI1

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
YIplac204-pOst1-ESCargo(Crimson)

Plasmid

#140154

Purpose

Expresses a vacuolar cargo for yeast at the TRP1 locus

Depositor

Benjamin Glick

Article

Casler et al Elife. 2020 Jun 25;9. pii: 56844. doi: 1

Insert

pOst1-APVNTT-DsRed-Express2-FKBP(FV)C22V-QRPL

Expression

Yeast

Promoter

TPI1

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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Y365A or A365Y inducible rescue

Plasmid

#131323

Purpose

gRNA to generate i-WT-r or i-MT-r systems

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

Y365A or A365Y inducible rescue

Use

CRISPR

Expression

Mammalian

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Rescue myc-PCDHGC5 mRNA

Plasmid

#122229

Purpose

Rescue mRNA for Protocadherin gamma C5 knock down with sh1 shRNA (plasmid 122227). With five silent mutations at sh1 shRNA target site.

Depositor

Angel de Blas

Article

Li et al J Neurosci. 2012 Aug 22;32(34):11780-97.

Insert

PCDHGC5 (Pcdhgc5 Rat)

Tags

9E10 cMyc epitope (EQKLISEEDL) was inserted betwe…

Expression

Mammalian

Mutation

Silent mutations are in five consecutive codons …

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pESC/mCitrine/erg3-mCherry

Plasmid

#117993

Purpose

Co-expression of mCitrine under the control of the Gal1 promoter and erg3 fused to mCherry (an ER-targeting marker protein) under the control of the Gal10 promoter

Depositor

Mathias Pribil

Article

Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

erg3-mCherry

Use

Plant/bacteria binary vector

Expression

Plant

Promoter

Gal10

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Torso 11.5 kb rescue fragment

Plasmid

#39810

Depositor

Norbert Perrimon

Article

Gayko et al Proc Natl Acad Sci U S A. 1999 Jan 19;96

Insert

Torso genomic DNA fragment (tor Fly)

Available Since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
pBluescript II NLS-MC-EGFP-tdTomato (pBET2)

Plasmid

#240411

Purpose

This plasmid facilitates the assessment of MMR proficiency in human living cells

Depositor

Isao Kuraoka

Article

Takedachi et al Mutat Res. 2022 Jan-Jun;824:111779. doi:

Insert

NLS-EGFP fusion protein

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-GalNAc-T2-GFP-ESCargo(FTV)

Plasmid

#140163

Purpose

Expresses a regulatable secretory cargo for mammalian cells and a Golgi marker

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

GalNAc-T2-msGFP2 and piGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V) (GALNT2 Synthetic, Human)

Tags

ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

Expression

Mammalian

Promoter

EF-1a

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF-pIgH-ESCargo(FTV,Crimson)

Plasmid

#140162

Purpose

Expresses a regulatable secretory cargo for mammalian cells

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

piGH-FTVNTT-E2-Crimson-FKBP(LV)(C22V)

Tags

ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

Expression

Mammalian

Promoter

EF-1a

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pESC-URA-Ub-G76A-Rnq1-GFP

Plasmid

#21052

Depositor

Judith Frydman

Article

Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

Insert

Rnq1 (RNQ1 Budding Yeast)

Use

Yeast/bacteria shuttle

Tags

GFP and ubiquitin

Expression

Yeast

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only
pEF-pIgH-ESCargo(FTV,Orange)

Plasmid

#140161

Purpose

Expresses a regulatable secretory cargo for mammalian cells

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

piGH-FTVNTT-E2-Orange-FKBP(LV)(C22V)

Tags

ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

Expression

Mammalian

Promoter

EF-1a

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac (GST-preSci site-CD45)

Plasmid

#177889

Purpose

Baculo expression of GST tag fused to human CD45 ICD

Depositor

Ron Vale

Article

Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

Insert

CD45 (aa 598-1304 only) (PTPRC Human)

Tags

TEV-GST-PreScission cut site

Expression

Insect

Mutation

L652P

Promoter

Polyhedrin

Available Since

Aug. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-TRIM24-F979A/N980A shRescue

Plasmid

#28143

Depositor

Michelle Barton

Insert

TRIM24 (TRIM24 Human)

Tags

FLAGX2

Expression

Mammalian

Mutation

A489V, F979A and N980A; aa 308 and 309 mutated fo…

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pRK5 HA-GST-Presc-mAkt1 (S473T)

Plasmid

#48806

Purpose

expresses mutant Akt1

Depositor

David Sabatini

Article

Kang et al Science. 2013 Jul 26;341(6144):1236566.

Insert

Akt1 (Akt1 Mouse)

Tags

HA-GST-Presc

Expression

Mammalian

Mutation

S473T

Promoter

CMV

Available Since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

Showing: 161 - 200 of 23042 results

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