Addgene

We narrowed to 2,785 results for: 2-Oct

Showing: 201 - 250 of 2785 results

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pHH0103_SRC-1/1-IS2/2

Plasmid

#91251

Purpose

Protein expression and purification of human SH3 domain construct SRC-1/1-IS2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SRC-1/1-IS2/2 (SRC Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_LASP1-1/1-SV1/2

Plasmid

#91399

Purpose

Protein expression and purification of human SH3 domain construct LASP1-1/1-SV1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

LASP1-1/1-SV1/2 (LASP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_SRC-1/1-IS1/2

Plasmid

#91349

Purpose

Protein expression and purification of human SH3 domain construct SRC-1/1-IS1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SRC-1/1-IS1/2 (SRC Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_DOCK5-1/1-IS1/2

Plasmid

#91303

Purpose

Protein expression and purification of human SH3 domain construct DOCK5-1/1-IS1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

DOCK5-1/1-IS1/2 (DOCK5 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_LASP1-1/1-SV2/2

Plasmid

#91392

Purpose

Protein expression and purification of human SH3 domain construct LASP1-1/1-SV2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

LASP1-1/1-SV2/2 (LASP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_ARHGAP10-1/1-SV2/2

Plasmid

#91436

Purpose

Protein expression and purification of human SH3 domain construct ARHGAP10-1/1-SV2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ARHGAP10-1/1-SV2/2 (ARHGAP10 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_MIA2-1/1-SV1/2

Plasmid

#91357

Purpose

Protein expression and purification of human SH3 domain construct MIA2-1/1-SV1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

MIA2-1/1-SV1/2 (MIA2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_CACNB3-1/1-SV1/2

Plasmid

#91336

Purpose

Protein expression and purification of human SH3 domain construct CACNB3-1/1-SV1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

CACNB3-1/1-SV1/2 (CACNB3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_MIA2-1/1-SV2/2

Plasmid

#91326

Purpose

Protein expression and purification of human SH3 domain construct MIA2-1/1-SV2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

MIA2-1/1-SV2/2 (MIA2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_DOCK5-1/1-IS2/2

Plasmid

#91304

Purpose

Protein expression and purification of human SH3 domain construct DOCK5-1/1-IS2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

DOCK5-1/1-IS2/2 (DOCK5 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_ABI2-1/1-SV1/2

Plasmid

#91285

Purpose

Protein expression and purification of human SH3 domain construct ABI2-1/1-SV1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ABI2-1/1-SV1/2 (ABI2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_ARHGAP10-1/1-SV1/2

Plasmid

#91286

Purpose

Protein expression and purification of human SH3 domain construct ARHGAP10-1/1-SV1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ARHGAP10-1/1-SV1/2 (ARHGAP10 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_CACNB3-1/1-SV2/2

Plasmid

#91290

Purpose

Protein expression and purification of human SH3 domain construct CACNB3-1/1-SV2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

CACNB3-1/1-SV2/2 (CACNB3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_MAPK8IP2-1/1-SV2/2

Plasmid

#91293

Purpose

Protein expression and purification of human SH3 domain construct MAPK8IP2-1/1-SV2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

MAPK8IP2-1/1-SV2/2 (MAPK8IP2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiCRISPR - Amplicon, BCR/ABL sgRNA 2

Plasmid

#70658

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic BCR/ABL-targeting sgRNA element from U6 promoter. Lentiviral backbone.

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

sgRNA against BCR/ABLamplicon found in CML-derived K562 cell line

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only
2842 pBS Bcl-2 5'genomic

Plasmid

#8747

Depositor

Stanley Korsmeyer

Article

Veis et al Cell 1993 Oct 22;75(2):229-40.

Insert

Bcl-2 5' genomic (Bcl2 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
LB124 (Bcl-2 from -1287 to -3934)

Plasmid

#15240

Depositor

Linda Boxer

Article

Wilson et al Mol Cell Biol. 1996 Oct . 16(10):5546-56

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial

Mutation

Bcl-2 fragment from -1287 to -3934 with P1

Promoter

Available since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
LB334 (Bcl-2 from -1287 to -1640)

Plasmid

#15241

Depositor

Linda Boxer

Article

Wilson et al Mol Cell Biol. 1996 Oct . 16(10):5546-56

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial

Mutation

Bcl-2 fragment from -1287 to -1640 with P1

Promoter

Available since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
LB360 (Bcl-2 from -1287 to -1337)

Plasmid

#15242

Depositor

Linda Boxer

Article

Wilson et al Mol Cell Biol. 1996 Oct . 16(10):5546-56

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial

Mutation

Bcl-2 fragment from -1287 to -1337 with P1

Promoter

Available since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
GB1-His-TEV-A7 (2-180)

Plasmid

#190066

Purpose

Codon-optimized bacterial expression plasmid. Expresses Annexin A7 proline rich domain.

Depositor

Lalit Deshmukh

Article

Yu et al Biochemistry. 2023 Oct 3. doi: 10.1021/a

Insert

Annexin A7 PRD (ANXA7 Human)

Use

Tags

GB1-His-TEV

Expression

Bacterial

Mutation

PRD only, residues 2-180

Promoter

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Mouse genome-wide lentiviral CRISPR gRNA library version 2

Pooled Library

#67988

Purpose

Mouse genome-wide CRISPR guide RNA library V2. (pKLV2-U6gRNA5(MouseV2)-PGKpuro2ABFP-W)

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Species

Homo sapiens, Mus musculus, and Synthetic

Use

CRISPR and Lentiviral

Available since

Oct. 14, 2016

Availability

Academic Institutions and Nonprofits only
pCDNA3-SREBP-2-GFP (A1134-1135)

Plasmid

#204203

Purpose

Mammalian expression of SREBP-2

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SREBP-2 (SREBF2 Human)

Use

Tags

T7x3 and eGFP

Expression

Mammalian

Mutation

Gly to Ala 1134, 1135

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 Flag beta-2-adrenergic-receptor

Plasmid

#14697

Depositor

Robert Lefkowitz

Article

Tang et al Proc Natl Acad Sci U S A. 1999 Oct 26. 9

Insert

B2AR (ADRB2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

June 19, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA4TO R99A CoV-2 nsp1 3xFLAG

Plasmid

#176061

Purpose

Expresses R99A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV-2 nsp1 (ORF1ab SARS-CoV-2)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-guide-puro mBltp1 (KIAA1109) - 2

Plasmid

#198490

Purpose

lentiviral stable expression of mBltp1 (KIAA1109) gRNA 2

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mBltp1 (4932438A13Rik Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-mDlx-GCaMP6f-Fishell-2

Plasmid

#83899

Purpose

GCaMP6f expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element

Depositor

Gordon Fishell

Article

Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

GCaMP6f

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

mDlx

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pVRC8400-SARS-CoV-2-NTD-AVI

Plasmid

#160475

Purpose

Expresses SARS-CoV-2 NTD domain with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

Insert

SARS-CoV-2-NTD

Use

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pVRC8400-SARS-CoV-2-RBD-AVI

Plasmid

#160476

Purpose

Expresses SARS-CoV-2 RBD domain with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

Insert

SARS-CoV-2-RBD

Use

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pVRC8400-SARS-CoV-2-S2P-AVI

Plasmid

#160474

Purpose

Expresses SARS-CoV-2 S2P protein with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

Insert

SARS-CoV-2-S2P

Use

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLQ2806-2 pHR: U6-Sasgv2TRE3G CMV-mCherry

Plasmid

#84250

Purpose

Expresses optimized Sa sgTRE3G gRNA with a mCherry fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sa sgTRE3G

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-bio-myc-mCE 2-210

Plasmid

#112715

Purpose

Expresses N-terminally bio-myc-tagged mouse CE/Rngtt 2-210 in mammalian cells

Depositor

Daniel Schoenberg

Article

Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

Insert

Rngtt (Rngtt Mouse)

Use

Tags

biotinylation signal peptide (Tagwerker et al. 20…

Expression

Mammalian

Mutation

Silent mutation at Arg 132 (CGT to CGC), deleted …

Promoter

CMV

Available since

Oct. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1-Teton-puro-shFDPS #2

Plasmid

#198760

Purpose

conditional knockdown of FDPS

Depositor

Hyonchol Jang

Article

Kim et al Exp Mol Med. 2018 Oct 17;50(10):137. doi

Insert

shFDPS #2 (FDPS Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSG5HA-MAGE-2-429

Plasmid

#89133

Purpose

mammalian expression of N-terminal HA-tagged full-length human MAGE-A11 amino acid residues 2-429

Depositor

Elizabeth Wilson

Article

Askew et al J Biol Chem. 2009 Dec 11;284(50):34793-8

Insert

MAGE-A11 (MAGEA11 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

contains aa 2-429

Promoter

SV40

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only
pLV_dCas9-ZIM3-KRAB_sgTAOK2i_#2

Plasmid

#172984

Purpose

CRISPRi for TAOK2

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

sgTAOK2i (TAOK2 Human)

Use

CRISPR and Lentiviral

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV_dCas9-KRAB_sgSTK4i_#2 (PuroR)

Plasmid

#209774

Purpose

CRISPRi for STK4

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

dCas9-KRAB, sgSTK4i (STK4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GB1-His-TEV-A7-Strep (2-180)

Plasmid

#190067

Purpose

Codon-optimized bacterial expression plasmid. Expresses Annexin A7 proline rich domain with strep.

Depositor

Lalit Deshmukh

Article

Yu et al Biochemistry. 2023 Oct 3. doi: 10.1021/a

Insert

Annexin A7 PRD (ANXA7 Human)

Use

Tags

GB1-His-TEV and Strep

Expression

Bacterial

Mutation

PRD only, residues 2-180

Promoter

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCG

Plasmid

#51476

Purpose

mammalian cell expression vector

Depositor

Winship Herr

Article

Tanaka et al Cell. 1990 Feb 9;60(3):375-86.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 28, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pSrpR-BCD7-tetR-ssrA P65U18C45T14 v40-2 (491)

Plasmid

#70736

Purpose

plasmid for building 3n2

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

tetR-ssrA

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pSrpR-BCD7-lambdaCI-ssrA P65U18C47T14 v40-2 (495)

Plasmid

#70735

Purpose

plasmid for building 5n1

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

lambdaCI-ssrA

Use

Tags

Expression

Mutation

Promoter

Available since

May 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pSrpR-BCD7-betI-ssrA P65U18C64T14 v50-2 (567)

Plasmid

#70734

Purpose

plasmid for building 3n1, 5n2

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

betI-ssrA

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBetI-BCD7-qacR-ssrA P69U18C63T16 v50-2 (568)

Plasmid

#70732

Purpose

plasmid for building 5n2

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

phlF-ssrA

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
ATF4 17: 5’ATF4.uORF1stopm1&2.GFP

Plasmid

#21863

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Stop codons between the colinear uORF1 and uORF2 …

Promoter

Available since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV_dCas9-KRAB_sgSTK3i_#2 (PuroR)

Plasmid

#209772

Purpose

CRISPRi for STK3

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

dCas9-KRAB, sgSTK3i (STK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCGN

Plasmid

#53395

Purpose

mammalian cell expression vector with HA-tag

Depositor

Winship Herr

Article

Tanaka et al Cell. 1990 Feb 9;60(3):375-86.

Insert

HA tag

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 28, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pVRC8400-SARS-CoV-2-RBD-SD1-AVI

Plasmid

#160477

Purpose

Expresses SARS-CoV-2 RBD-SD1 domain with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

Insert

SARS-CoV-2-RBD-SD1

Use

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLQ1852-2 pHR: U6-SpsgCD95-1 CMV-EGFP

Plasmid

#84251

Purpose

Expresses Sp sgCD95-1 gRNA with an EGFP fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sp sgCD95-1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLQ2853-4 pHR: U6-Sasgv2CXCR4-2 CMV-EGFP

Plasmid

#84255

Purpose

Expresses optimized Sa sgCXCR4-2 gRNA with an EGFP fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sa sgCXCR4-2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLQ1852-3 pHR: U6-SpsgCD95-2 CMV-EGFP

Plasmid

#84252

Purpose

Expresses Sp sgCD95-2 gRNA with an EGFP fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sp sgCD95-2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-H1-shSRGAP2B/C#2-hsynapsin-EGFP

Plasmid

#220797

Purpose

shRNA plasmid against human SRGAP2B/C genes (#2) with EGFP expression in neurons

Depositor

Pierre Vanderhaeghen

Article

Libe-Philippot et al Neuron. 2024 Oct 9:S0896-6273(24)00645-7

Insert

EGFP

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA4TO K164A H165A CoV-2 nsp1 3xFLAG

Plasmid

#176060

Purpose

Expresses K164A K165A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV-2 K164A H165A nsp1 (ORF1ab SARS-CoV-2)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

K164A and H165A

Promoter

CMV

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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