We narrowed to 2,187 results for: D10

1. pD10AH840AhCas9 (GB1041)

   Plasmid

   #68207

   Purpose

   Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed; human codon optimis…

   Promoter

   Available since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. iPEmaxΔR-D10A

   Plasmid

   #239381

   Purpose

   For inverse prime editor using nCas9-D10A-R221K-N394K and M-MLV-RTΔRNase H in HEK293T cells

   Depositor

   Caixia Gao

   Insert

   nCas9(D10A-R221K-N394K), M-MLV-RT(ΔRNase H)
   

   Use

   CRISPR

   Tags

   BPNLS and SV40 NLS, c-myc NLS

   Expression

   Mammalian

   Mutation

   D10A, R221K, N394K in Cas9; ΔRNase H in M-MLV RT

   Promoter

   CMV

   Available since

   Aug. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. iPEmax-D10A

   Plasmid

   #239386

   Purpose

   For inverse prime editor using nCas9-D10A-R221K-N394K in HEK295T cells

   Depositor

   Caixia Gao

   Insert

   nCas9-D10A-R221K-N394K, M-MLV-RT
   

   Use

   CRISPR

   Tags

   BPNLS and SV40 NLS, c-myc NLS

   Expression

   Mammalian

   Mutation

   D10A, R221K, N394K in Cas9

   Promoter

   CMV

   Available since

   Aug. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. D10A-Cas9

   Plasmid

   #239383

   Purpose

   For circular RNA-mediated inverse prime editor using nCas9-D10A in HEK294T cells

   Depositor

   Caixia Gao

   Insert

   nCas9-D10A
   

   Use

   CRISPR

   Tags

   BPNLS and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A in Cas9

   Promoter

   CMV

   Available since

   Aug. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR221-STARD10

   Plasmid

   #166216

   Purpose

   Gateway entry vector for N. furzeri STARD10

   Depositor

   Daniel Jarosz

   Article

   Chen et al Dev Cell. 2024 Jul 22;59(14):1892-1911.e

   Insert

   STARD10
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBP-TagR_MCD10

   Plasmid

   #204101

   Purpose

   Type 0 tag-compatible RBS part

   Depositor

   Mikhail Shapiro

   Insert

   MCD10 medium RBS for assembly with tag part
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBP-R_MCD10

   Plasmid

   #204093

   Purpose

   Type 0 RBS part

   Depositor

   Mikhail Shapiro

   Insert

   MCD10 medium RBS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAD100_hIRG1_4-461_pvp008

   Plasmid

   #124875

   Purpose

   Expresses hCAD Arg273His mutant in E.coli

   Depositor

   Konrad Buessow

   Article

   Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

   Insert

   cis-aconitate decarboxylase (ACOD1 Human)
   

   Use

   Tags

   StrepTag and TEV site

   Expression

   Bacterial

   Mutation

   mutated Arg273 to His, deleted amino acids 1-3 an…

   Promoter

   T7

   Available since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQ151D10A-x3.7

   Plasmid

   #124309

   Purpose

   Gateway entry vector with pcoCas9D10A-x3.7 (Plant codon optimized)

   Depositor

   Yiping Qi

   Article

   Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

   Insert

   pcoCas9D10A-x3.7
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAtCas9-D10A_1

    Plasmid

    #91159

    Purpose

    Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:hpt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    AtCas9_D10A (nickase)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A

    Promoter

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. Syntaxin-4 d10

    Plasmid

    #44188

    Depositor

    Thomas Weimbs

    Article

    Reales et al PLoS One. 2011;6(6):e21181. doi: 10.1371

    Insert

    Syntaxin-4
    

    Use

    Tags

    2xMyc and His

    Expression

    Mammalian

    Mutation

    deleted first 10 amino acids

    Promoter

    Available since

    July 1, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pGEM-T ZfHoxd10a

    Plasmid

    #27543

    Depositor

    Victoria Prince

    Insert

    Hoxd10a (hoxd10a Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pUNIV-SIG-5HTR4D100A

    Plasmid

    #16312

    Depositor

    Bruce Conklin

    Article

    Hsiao et al Proc Natl Acad Sci U S A. 2008 Jan 29. 1

    Insert

    h5HT4b Serotonin Receptor (Rs1 D100A RASSL) (HTR4 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Added FLAG tag; Aspartic Acid 100 changed to Alan…

    Promoter

    Available since

    Jan. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

14. lentiCas9n(D10A)-Blast

    Plasmid

    #63593

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Inserts

    Cas9
    Blasticidin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A, nickase mutant

    Promoter

    EFS-NS

    Available since

    April 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDD104 (Peft-3::Cre)

    Plasmid

    #47551

    Purpose

    Cre recombinase expression plasmid for removal of floxed selectable markers in the C. elegans germline

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

    Insert

    Cre Recombinase
    

    Use

    Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    eef-1A.1 (eft-3)

    Available since

    Sept. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPD100 VP16-ZF43

    Plasmid

    #138761

    Purpose

    Constitutive expression of VP16-ZF43 under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    VP16-ZF43
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRS415_pGal-nCas9(D10A)

    Plasmid

    #79618

    Purpose

    Expresses nCas9(D10A) in yeast cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    D10A for nickase Cas9

    Promoter

    pGal1

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDe-CAS9-D10A

    Plasmid

    #61477

    Purpose

    based on pDe-CAS9, encodes Cas9 D10A nickase variant

    Depositor

    Holger Puchta

    Article

    Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

    Insert

    Cas9-D10A
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    catalytic Asp10 changed to Ala

    Promoter

    PcUbi4-2

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. Rad51–Cas9(D10A)

    Plasmid

    #125567

    Purpose

    Expresses the Rad51–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

    Depositor

    David Liu

    Article

    Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

    Insert

    Rad51–Cas9(D10A) (RAD51 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pST1374-GCN4-D10A

    Plasmid

    #113022

    Purpose

    Expresses GCN4-D10A in mammalian cells

    Depositor

    Xingxu Huang

    Article

    Jiang et al Cell Res. 2018 Jun 6. pii: 10.1038/s4142

    Insert

    GCN4-D10A (GCN4 Budding Yeast, S. pyogenes)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CMV

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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