Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 6,291 results for: feng zhang

Clear keywords
Showing: 81 - 100 of 6291 results

  1. AAV:ITR-U6-sgRNA(NeuN)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

    Plasmid
    #60230
    Purpose
    Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Cre recombinase
    EGFP
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA-P2A and EGFP-KASH
    Expression
    Mammalian
    Promoter
    U6 and hSyn
    Available Since
    Nov. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    60230-plasmid-map-sequence-id-490567

    Enlarge

  5. pC0048-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q)

    Plasmid
    #103864
    Purpose
    dPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Longer linker than pC0039.
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GSGGGGS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105ā€¦
    Available Since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    103864-plasmid-map-sequence-id-199176

    Enlarge

  11. pPbcas13b

    Plasmid
    #89906
    Purpose
    Bacterial expression for pbCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.
    Depositor
    Feng Zhang
    Article
    Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.
    Insert
    Cas13b
    Expression
    Bacterial
    Promoter
    Lac
    Available Since
    May 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    89906-plasmid-map-sequence-id-178224

    Enlarge

  16. AAV:ITR-U6-sgRNA(LacZ)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR

    Plasmid
    #60225
    Purpose
    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA targeting LacZ. AAV backbone.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Renilla luciferase
    Cre recombinase
    Use
    AAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targeā€¦
    Tags
    Cre-HA and Rluc-P2A
    Expression
    Mammalian
    Promoter
    EFS and U6
    Available Since
    Oct. 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    60225-plasmid-map-sequence-id-96391

    Enlarge

  20. PX383 Lactobacillus johnsonii Cas9

    Plasmid
    #68320
    Purpose
    Lactobacillus johnsonii Cas9
    Depositor
    Feng Zhang
    Insert
    LjCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    68320-plasmid-map-sequence-id-119535

    Enlarge

Showing: 81 - 100 of 6291 results