We narrowed to 3,550 results for: golden gate

1. L0I87-SPCHPromoter_GG0-1

   Plasmid

   #219707

   Purpose

   Level 0 pSPCH promoter, GG0 and GG1 BsaI sites for golden gate assembly.

   Depositor

   Jennifer Nemhauser

   Insert

   GG0-pSPCH-GG1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   WT promoter with added golden gate linkers

   Promoter

   Available since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. L0I84 - GG0-pAHP6-GG1

   Plasmid

   #219706

   Purpose

   Level 0 pAHP6 promoter, GG0 and GG1 BsaI sites for golden gate assembly.

   Depositor

   Jennifer Nemhauser

   Insert

   GG0-pAHP6-GG1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   WT promoter with added golden gate linkers

   Promoter

   Available since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTALE64G

   Plasmid

   #35188

   Purpose

   Golden Gate Compatible TALE Transcription Factor using VP64 Domain with T2A-GFP addition

   Depositor

   Gang Bao

   Insert

   TAL Effector-VP64-T2A-eGFP
   

   Use

   TALEN

   Tags

   Expression

   Mammalian

   Mutation

   +63 C Terminus, VP64 Domain, T2A-GFP

   Promoter

   CMV

   Available since

   Dec. 12, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pTALE64

   Plasmid

   #35189

   Purpose

   Golden Gate Compatible TALE Transcription Factor using VP64 Domain

   Depositor

   Gang Bao

   Insert

   Transcription Activator Like Effector Transcription Factor
   

   Use

   TALEN

   Tags

   Expression

   Mammalian

   Mutation

   +63 C Terminus, VP64 Domain

   Promoter

   CMV

   Available since

   Dec. 12, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pOGG012

   Plasmid

   #113986

   Purpose

   Vector construction module. Partition genes (parABCDE) for plasmid stability in absence of antibiotic selection for construction of Golden Gate cloning vectors

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Insert

   Partition genes (parABCDE)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Promoter

   Available since

   Jan. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOGG010

   Plasmid

   #113984

   Purpose

   Vector construction module. RK2 (IncP) low-copy number origin of replication for construction of Golden Gate cloning vectors

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Insert

   RK2 (IncP)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Promoter

   Available since

   Jan. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOGG011

   Plasmid

   #113985

   Purpose

   Vector construction module. pBBR1 medium-copy number origin of replication for construction of Golden Gate cloning vectors

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Insert

   pBBR1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Promoter

   Available since

   Jan. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. High Complexity Golden Gate Assembly Standards Kit (24 fragment lac cassette)

   Plasmid Kit

   #1000000221

   Purpose

   A positive control for high-complexity golden gate assembly. Junction sites were predicted using comprehensive end-joining ligation fidelity and bias data to allow high accuracy assembly.

   Depositor

   Eric Cantor

   Available since

   June 1, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pOPARA2

   Plasmid

   #200417

   Purpose

   Golden Gate assembly of expression constructs containing pBAD promoter (spectinomycin resistance)

   Depositor

   Kamil Witek

   Article

   Lesley et al bioRxiv

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOPARA1

    Plasmid

    #200416

    Purpose

    Golden Gate assembly of expression constructs containing pBAD promoter (carbenicillin resistance)

    Depositor

    Kamil Witek

    Article

    Lesley et al bioRxiv

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TALEN_C63

    Plasmid

    #40788

    Purpose

    Golden Gate Compatible TALEN Construct for site specific modification of genome, +63 C Terminus, Codon Optimized FokI

    Depositor

    Gang Bao

    Insert

    Transcription Activator Like Effector Nuclease
    

    Use

    TALEN

    Tags

    Expression

    Mammalian

    Mutation

    +63 C Terminus, Codon Optimized FokI Endonuclease

    Promoter

    CMV

    Available since

    Dec. 12, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pCVL RipTAL(Nd154C63) SFFV Xba-Sal linker

    Plasmid

    #50627

    Purpose

    pRipTAL plasmid compatible with Golden Gate TALEN cloning system. For cloning of megaTALs or other TAL-fusions and expression in mammalian cells. Meganuclease/fusion is cloned between Xba-Sal sites.

    Depositor

    Andrew Scharenberg

    Type

    Empty backbone

    Use

    Lentiviral and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. TALEN_C18

    Plasmid

    #47974

    Purpose

    Golden Gate Compatible TALEN Construct site specific modification of genome, shortened C Terminus, Codon Optimized FokI

    Depositor

    Gang Bao

    Insert

    Transcription Activator Like Effector Nuclease
    

    Use

    TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Shortened C-Terminus,Codon Optimized FokI Endonuc…

    Promoter

    CMV

    Available since

    Dec. 12, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pNDGG014

    Plasmid

    #231335

    Purpose

    BEVA Golden Gate cloning vector; Narrow host range plasmid. L1-pMB1-Gm-ELT3; pNDGG021, pOGG009, pNDGG007, pOGG013. GmR.

    Depositor

    Barney Geddes

    Article

    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pQGG003

    Plasmid

    #231330

    Purpose

    BEVA Golden Gate cloning vector; BEVA2.0 Narrow host range plasmid with I-SceI counterselectable site for BpiI cloning; KmR/NmR.

    Depositor

    Barney Geddes

    Article

    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNIC-NoTag-GG

    Plasmid

    #215810

    Purpose

    Cloning of target gene with no tags

    Depositor

    Frank von Delft, ASAP Discovery

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pET-HIS-Sangamo

    Plasmid

    #40786

    Purpose

    for e. coli expression, Golden Gate Compatible TALEN incorporated with N Terminal 6xHIS Tag, Codon Optimized FokI

    Depositor

    Gang Bao

    Insert

    Transcription Activator Like Effector Nuclease
    

    Use

    TALEN

    Tags

    N Terminal 6xHIS

    Expression

    Bacterial

    Mutation

    +63 C Terminus, Codon Optimized FokI Endonuclease

    Promoter

    T7

    Available since

    Dec. 12, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pET-Sangamo-HIS

    Plasmid

    #40787

    Purpose

    for e. coli expression, Golden Gate Compatible TALEN incorporated with C Terminal 6xHIS Tag, Codon Optimized FokI

    Depositor

    Gang Bao

    Insert

    Transcription Activator Like Effector Nuclease
    

    Use

    TALEN

    Tags

    C Terminal 6xHIS

    Expression

    Bacterial

    Mutation

    +63 C Terminus,Codon Optimized FokI Endonuclease

    Promoter

    T7

    Available since

    Dec. 12, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pNDGG003

    Plasmid

    #231316

    Purpose

    BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RK2-Tc-par-ELT4; GG assembly from pOGG010, pOGG012, pOGG014, pOGG042, and pOGG004, TcR

    Depositor

    Barney Geddes

    Article

    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNDGG050

    Plasmid

    #231319

    Purpose

    BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RK2-Gm-par-ELT4; GG assembly from pOGG004, pOGG009, pOGG010, pOGG012, and pOGG014, GmR

    Depositor

    Barney Geddes

    Article

    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge