Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 5,553 results for: golden gate

Clear keywords
Showing: 81 - 100 of 5553 results

  1. pTALEN(Nd154C63) SFFV

    Plasmid
    #50431
    Purpose
    pTAL plasmid compatible with the Golden Gate TALEN cloning system, for cloning of TALENs and expression in mammalian cells.
    Depositor
    Andrew Scharenberg
    Type
    Empty backbone
    Use
    Lentiviral and TALEN
    Expression
    Mammalian
    Promoter
    SFFV
    Available since
    May 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    50431-plasmid-map-sequence-id-75352

    Enlarge

  2. EcoFlex MoClo Kit

    Plasmid kit
    #1000000080
    Purpose
    Collection of DNA parts for synthetic biology use in E. coli, cell-free protein synthesis, protein purification, and hierarchical assembly of transcription units.
    Depositor
    Paul Freemont
    Application
    Cloning and Synthetic Biology
    Vector type
    Bacterial Expression
    Cloning type
    Golden Gate (MoClo)
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  3. CIDAR MoClo Parts Kit

    Plasmid kit
    #1000000059
    Purpose
    Collection of standardized parts for combinatorial design and expression tuning in E. coli.
    Depositor
    Douglas Densmore
    Application
    Cloning and Synthetic Biology
    Vector type
    Bacterial Expression
    Cloning type
    Golden Gate (MoClo)
    Available since
    Jan. 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  4. zCas9i cloning kit: MoClo-compatible CRISPR/Cas9 cloning kit with intronized Cas9

    Plasmid kit
    #1000000171
    Purpose
    MoClo-compatible vectors for assembly of CRISPR/Cas9 constructs using a high efficiency intron-optimized SpCas9-coding gene in plants.
    Depositor
    Sylvestre Marillonnet, Johannes Stuttmann
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector type
    Plant Expression
    Editing type
    CRISPR
    Cloning type
    Golden Gate (MoClo)
    Available since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only

  5. MoClo CRISPR/Cas Toolkit for Plants

    Plasmid kit
    #1000000159
    Purpose
    Plasmids for gene editing using Cas nucleases and base editors in monocots and dicots.
    Depositor
    Vladimir Nekrasov
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector type
    Plant Expression
    Editing type
    CRISPR
    Cloning type
    Golden Gate (MoClo)
    Available since
    July 17, 2020
    Availability
    Academic Institutions and Nonprofits only

  6. PGR-Blue

    Plasmid
    #68374
    Purpose
    Terminator quantification vector utilizing Golden Gate Assembly and amilCP. Positive colonies are green and false positives are blue. Terminators are placed between GFP and RFP - induced by arabinose.
    Depositor
    Nathan Reyna
    Type
    Empty backbone
    Use
    Synthetic Biology; Golden gate assembly
    Expression
    Bacterial
    Promoter
    pBAD
    Available since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    68374-plasmid-map-sequence-id-121945

    Enlarge

  7. pET-Sangamo-HIS

    Plasmid
    #40787
    Purpose
    for e. coli expression, Golden Gate Compatible TALEN incorporated with C Terminal 6xHIS Tag, Codon Optimized FokI
    Depositor
    Gang Bao
    Insert
    Transcription Activator Like Effector Nuclease
    Use
    TALEN
    Tags
    C Terminal 6xHIS
    Expression
    Bacterial
    Mutation
    +63 C Terminus,Codon Optimized FokI Endonuclease
    Promoter
    T7
    Available since
    Dec. 12, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    40787-plasmid-map-sequence-id-68902

    Enlarge

  8. pTALEN(Nd154C63) MND

    Plasmid
    #50432
    Purpose
    pTAL plasmid compatible with the Golden Gate TALEN cloning system, for cloning of TALENs and expression in mammalian cells.
    Depositor
    Andrew Scharenberg
    Type
    Empty backbone
    Use
    Lentiviral and TALEN
    Expression
    Mammalian
    Promoter
    MND
    Available since
    May 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    50432-plasmid-map-sequence-id-75353

    Enlarge

  9. pTALEN(Nd154C63) Ef1a

    Plasmid
    #50433
    Purpose
    pTAL plasmid compatible with the Golden Gate TALEN cloning system, for cloning of TALENs and expression in mammalian cells.
    Depositor
    Andrew Scharenberg
    Type
    Empty backbone
    Use
    Lentiviral and TALEN
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    May 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    50433-plasmid-map-sequence-id-75354

    Enlarge

  10. pTALE64G

    Plasmid
    #35188
    Purpose
    Golden Gate Compatible TALE Transcription Factor using VP64 Domain with T2A-GFP addition
    Depositor
    Gang Bao
    Insert
    TAL Effector-VP64-T2A-eGFP
    Use
    TALEN
    Expression
    Mammalian
    Mutation
    +63 C Terminus, VP64 Domain, T2A-GFP
    Promoter
    CMV
    Available since
    Dec. 12, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    35188-plasmid-map-sequence-id-42839

    Enlarge

  11. pTALE64

    Plasmid
    #35189
    Purpose
    Golden Gate Compatible TALE Transcription Factor using VP64 Domain
    Depositor
    Gang Bao
    Insert
    Transcription Activator Like Effector Transcription Factor
    Use
    TALEN
    Expression
    Mammalian
    Mutation
    +63 C Terminus, VP64 Domain
    Promoter
    CMV
    Available since
    Dec. 12, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    35189-plasmid-map-sequence-id-68899

    Enlarge

  12. TALEN_C63

    Plasmid
    #40788
    Purpose
    Golden Gate Compatible TALEN Construct for site specific modification of genome, +63 C Terminus, Codon Optimized FokI
    Depositor
    Gang Bao
    Insert
    Transcription Activator Like Effector Nuclease
    Use
    TALEN
    Expression
    Mammalian
    Mutation
    +63 C Terminus, Codon Optimized FokI Endonuclease
    Promoter
    CMV
    Available since
    Dec. 12, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    40788-plasmid-map-sequence-id-68904

    Enlarge

  13. pICSL12013

    Plasmid
    #245630
    Purpose
    Level 0 Golden Gate part 6GAL4UAS promoter, GGAG - AATG
    Depositor
    Mark Youles
    Insert
    6GAL4UAS promoter
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245630-plasmid-map-sequence-id-495460

    Enlarge

  14. pICSL50020

    Plasmid
    #245582
    Purpose
    Level 0 Golden Gate part C-Terminal Tag with overhangs TTCG - GCTT
    Depositor
    Mark Youles
    Insert
    C-Terminal tag eCFP
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245582-plasmid-map-sequence-id-495505

    Enlarge

  15. pICSL50068

    Plasmid
    #245623
    Purpose
    Level 0 Golden Gate part C-Terminal Tag with overhangs TTCG - GCTT
    Depositor
    Mark Youles
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    C-Terminal tag mTurquoise2 (monomeric)
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245623-plasmid-map-sequence-id-495482

    Enlarge

  16. pICSL12081

    Plasmid
    #245651
    Purpose
    Level 0 Golden Gate part pLAT52 Pollen Specific promoter, GGAG - AATG
    Depositor
    Mark Youles
    Insert
    pLAT52 Pollen Specific promoter
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available since
    Jan. 5, 2026
    Availability
    Academic Institutions and Nonprofits only
    245651-plasmid-map-sequence-id-495392

    Enlarge

  17. pICSL12030

    Plasmid
    #245637
    Purpose
    Level 0 Golden Gate part pAt1: Tip Delta (AT3g16240) promoter, GGAG - AATG
    Depositor
    Mark Youles
    Insert
    pAt1: Tip Delta (AT3g16240) promoter
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available since
    Jan. 5, 2026
    Availability
    Academic Institutions and Nonprofits only
    245637-plasmid-map-sequence-id-495381

    Enlarge

  18. pICSL13003

    Plasmid
    #245653
    Purpose
    Level 0 Golden Gate part 6GAL4UAS (Dex inducible expression system) promoter, GGAG - CCAT
    Depositor
    Mark Youles
    Insert
    6GAL4UAS (Dex inducible expression system) promoter
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    245653-plasmid-map-sequence-id-495394

    Enlarge

  19. pICSL60038

    Plasmid
    #245677
    Purpose
    Level 0 Golden Gate partAtUbi10 terminator, GCTT - CGCT
    Depositor
    Mark Youles
    Insert
    AtUbi10 terminator
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245677-plasmid-map-sequence-id-495499

    Enlarge

  20. pICSL41308_CP

    Plasmid
    #245695
    Purpose
    Level 0 Golden Gate Coding Sequence (WITH stop codon) acceptor with AmilCP negative selection cassette AATG - GCTT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available since
    Dec. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    245695-plasmid-map-sequence-id-495761

    Enlarge

Showing: 81 - 100 of 5553 results