We narrowed to 664 results for: rtta

1. AAVS1 CAG rtTA3 TauP301L 2N4R-EGFP

   Plasmid

   #132393

   Purpose

   Exchange cassette containing inducible TauP301L 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site

   Depositor

   Gerold Schmitt-Ulms

   Article

   Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

   Insert

   human TauP301L 2N4R-EGFP (MAPT Human, Synthetic)
   

   Use

   CRISPR and Cre/Lox

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Proline 301 to Leucine (P301L)

   Promoter

   TREtight

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAVS1-Neo-TRE-CMV-Cre-rtTA

   Plasmid

   #165457

   Purpose

   Tetracycline inducible CRE expression from AAVS1 locus

   Depositor

   Madeline Lancaster

   Article

   Benito-Kwiecinski et al Cell. 2021 Apr 15;184(8):2084-2102.e19.

   Insert

   CRE
   

   Expression

   Mammalian

   Promoter

   Tight TRE promoter

   Available Since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAVS1 CAG rtTA3 TauWT 2N4R-EGFP

   Plasmid

   #132389

   Purpose

   Exchange cassette containing inducible TauWT 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site

   Depositor

   Gerold Schmitt-Ulms

   Article

   Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

   Insert

   human TauWT 2N4R-EGFP (MAPT Human, Synthetic)
   

   Use

   CRISPR and Cre/Lox

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   TREtight

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Pblu-AAVS1-Cas9-p300-M2rtTA-AAVS1

   Plasmid

   #112261

   Purpose

   Donor plasmid with a Cas9-p300 expression cassette, a M2rtTA expression cassette, a T2A-puro selection cassette, and two gRNA recognition sequences at both ends of the whole insertion DNA fragment.

   Depositor

   Zhili Rong

   Article

   Ma et al FEBS Lett. 2018 Jun 5. doi: 10.1002/1873

   Insert

   spCas9-p300 (EP300 Human, Synthetic)
   

   Expression

   Mammalian

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS-TRE-FHDnd1-Ubc-rtTA-I2G

   Plasmid

   #70076

   Purpose

   2nd gen lentiviral vector. tet/dox controllable expression of FLAG-HA-tagged murine Dnd1 in mammalian cells

   Depositor

   Thomas Tuschl

   Article

   Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

   Insert

   Dnd1 (Dnd1 Mouse)
   

   Use

   Lentiviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Silent mutations are introduced to escape from RN…

   Promoter

   Tet responsible promoter

   Available Since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRRL-SFFV-rtTA3-IRES-Luc-P2A-mtagBFP

   Plasmid

   #176027

   Purpose

   construct expresses the reverse tet-responsive transactivator 3 (rtTA3) together with Luciferase (Luc) for bioluminescence in vivo imaging, and mTaqBFP for enriching transgenic cells by flow cytometry

   Depositor

   Irmela Jeremias

   Article

   Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

   Inserts

   rtTA3
   Luc2
   mtagBFP
   

   Use

   Lentiviral

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBSKΔB- CAG-rtTA2sM2-IRES-tTSkid-IRES-Neo

   Plasmid

   #62346

   Purpose

   Expresses rtTA2sM2 and tTSkid in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Nucleic Acids Res. 2015 Jun 19. pii: gkv

   Insert

   rtTA2sM2-IRES-tTSkid
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAVS1-TRE-Cas9- puro-polyA-CAG-rtTA

   Plasmid

   #107270

   Purpose

   Expresses Cas9 upon induction with doxicycline.

   Depositor

   Pablo Menéndez

   Article

   Castano et al Stem Cell Res. 2017 May;21:137-140. doi:

   Inserts

   hSpCas9
   rtTA
   bGH polyA-SV40polyA
   

   Tags

   3xFlag, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Promoter

   CAG and TRE

   Available Since

   April 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAVS1-Neo-CAG-M2rtTA-H2BmNeon (donorAB-mNeon)

   Plasmid

   #85799

   Purpose

   donorAB-Neon targeting vector for human AAVS1 locus; knock-in of Dox controlled H2BGFP

   Depositor

   Roland Friedel

   Article

   Tejero et al EBioMedicine. 2019 Apr 2. pii: S2352-396

   Insert

   PPP1R12C (PPP1R12C Human)
   

   Use

   Human targeting

   Mutation

   homology arms for knock-in into first intron

   Available Since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCS-TRE-FHDnd1-Y102A-Ubc-rtTA-I2G

    Plasmid

    #70075

    Purpose

    2nd gen lentiviral vector. tet/dox controllable expression of FLAG-HA-tagged murine Dnd1 (RRM mutant) in mammalian cells

    Depositor

    Thomas Tuschl

    Article

    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

    Insert

    Dnd1 (Dnd1 Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Y102A. Silent mutations are introduced to escape …

    Promoter

    Tet responsible promoter

    Available Since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. dnCEBP: pLenti-TRE-A-C/EBP-PGK-rtTAA-2A-Puro

    Plasmid

    #177201

    Purpose

    encoding a dominant negative form of CEBP under inducible promoter

    Depositor

    Markus Covert

    Article

    Kudo et al bioRxiv (2022)

    Insert

    dnCEBP (A-C/EBP)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. tre-stblHIF: pLenti-TRE-stblHIF-PGK-rtTAA-2A-Puro

    Plasmid

    #177203

    Purpose

    encoding stblHIF under inducible promoter

    Depositor

    Markus Covert

    Article

    Kudo et al bioRxiv (2022)

    Insert

    stblHIF
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    P402A/P577G/N813A

    Promoter

    TRE

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. tetO-MyoD-T2A-GFPnls-mPGK-rtTA-IRES-Puro

    Plasmid

    #69546

    Purpose

    Tet-On system expressing MyoD-2A-GFP-2xNLS.

    Depositor

    Charles Gersbach

    Inserts

    eGFP
    Myod1 (Myod1 Mouse)
    

    Use

    Lentiviral

    Tags

    2x nuclear localization sequence and T2A "sk…

    Mutation

    Removed stop codon to fuse it to the 2A-eGFPnls i…

    Available Since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAVS1-Neo-UBC-M2rtTA-H2BmNeon (donorAB-UBC-mNeon)

    Plasmid

    #85800

    Purpose

    donorAB-UBC-Neon targeting vector for human AAVS1 locus; knock-in of Dox controlled H2Bneon

    Depositor

    Roland Friedel

    Article

    Tejero et al EBioMedicine. 2019 Apr 2. pii: S2352-396

    Insert

    PPP1R12C (PPP1R12C Human)
    

    Use

    Human targeting

    Mutation

    homology arms for knock-in into first intron

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. LV-TRE-MyoD-Stop-LacZ PGK-rtTA-IRES-Puro

    Plasmid

    #61441

    Purpose

    Dox-inducible MyoD with mRNA tag for smFISH

    Depositor

    Charles Gersbach

    Article

    Gibson et al Integr Biol (Camb). 2015 Jun 8;7(6):663-

    Insert

    MyoD (Myod1 Mouse)
    

    Use

    Lentiviral

    Available Since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB_rtTA_BsmBI

    Plasmid

    #126028

    Purpose

    PiggyBac cargo vector expressing rtTA, contains BsmBI sites for sgRNA cloning

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

    Type

    Empty backbone

    Use

    CRISPR; Piggybac

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV_hEF1a_rtTA3

    Plasmid

    #61472

    Purpose

    3rd generation lentiviral vector; constitutive expression of rtTA3 (TetON system)

    Depositor

    Ron Weiss

    Article

    Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

    Insert

    rtTA3
    

    Use

    Lentiviral

    Promoter

    hEF1a

    Available Since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAVS1_Puro_ccdb_Ubc_rtTA

    Plasmid

    #172130

    Purpose

    Destination vector for AAVS1 targeting

    Depositor

    Kenji Kontani

    Article

    Araki et al J Biol Chem. 2021 Nov 18:101428. doi: 10

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    PGK1

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. MXS_rtTA3

    Plasmid

    #62428

    Purpose

    MXS_chaining vector with rtTA3

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    Reverse tetracycline transactivator
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSLQ8702 pHR-TRE3G-AcrVA1-EF1a-sfGFP-T2A-rtta-WPRE

    Plasmid

    #140229

    Purpose

    Dox-inducible expression of anti-Cpf1 AcrVA1. Constitutive EF1a driven rtTA and GFP

    Depositor

    Stanley Qi

    Article

    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

    Insert

    AntiCRISPR protein AcrVA1
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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