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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,160 results for: ung

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Showing: 81 - 100 of 4160 results

  1. pFC334

    Plasmid
    #87846
    Purpose
    AMA1 plasmid with Aspergillus optimized Cas9, argB selection marker and yA specific sgRNA expressed with ribozymes
    Depositor
    Uffe Mortensen
    Article
    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do
    Inserts
    Cas9
    argB
    yA specific sgRNA
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Promoter
    A. nidulans gpdA promoter with Hammerhead ribozym…
    Available Since
    May 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    87846-plasmid-map-sequence-id-174613

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  5. BPK4410 - human expression plasmid for SpCas9 Cluster 1 (HypaCas9)

    Plasmid
    #101178
    Purpose
    Human expression plasmid for SpCas9 Cluster 1 variant (HypaCas9): CMV-T7-hSpCas9-Cluster1(N692A, M694A, Q695A, H698A)-NLS(SV40)-3xFLAG
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-Cluster1(N692A/M694A/Q695A/H698A)
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    N692A/M694A/Q695A/H698A
    Promoter
    CMV
    Available Since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101178-plasmid-map-sequence-id-219907

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  6. pCAG-CBE4max-SpG-P2A-EGFP (RTW4552)

    Plasmid
    #139998
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized CBE4max SpCas9 variant named SpG with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…
    Promoter
    CAG
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139998-plasmid-map-sequence-id-394975

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  9. pMA3790

    Plasmid
    #70110
    Purpose
    mtDNA elimination/production of rho-0 cells
    Depositor
    Mikhail Alexeyev
    Article
    Spadafora et al PLoS One. 2016 May 2;11(5):e0154684. doi
    Inserts
    EGFP
    hUNG1 Y147A
    Neo
    Tags
    None, human OTC mitochondrial targeting sequence,…
    Expression
    Mammalian
    Mutation
    Y147A, aa's 1-77 removed
    Promoter
    CMV, EF1a, and SV40 and native
    Available Since
    May 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    70110-plasmid-map-sequence-id-399654

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  16. pCAG-CBE4max-SpG-P2A-EGFP (RTW4552)

    Plasmid
    #139998
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized CBE4max SpCas9 variant named SpG with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…
    Promoter
    CAG
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139998-plasmid-map-sequence-id-394975

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  17. MSP469

    Plasmid
    #65771
    Purpose
    Human expression vector for SpCas9 VQR variant: CMV-T7-humanSpCas9(D1135V/R1335Q/T1337R)-NLS-3xFLAG (VQR variant)
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/R1335Q/T1337R)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135V, R1335Q, and T1337R mutations in Cas9
    Promoter
    CMV & T7
    Available Since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65771-plasmid-map-sequence-id-112653

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Showing: 81 - 100 of 4160 results