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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,485 results for: 683

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Showing: 1961 - 2000 of 2485 results

  8. TFORF1344

    Plasmid
    #143792
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    FEZF2 (FEZF2 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    143792-plasmid-map-sequence-id-396243

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  11. pCW-GemininΔDbox-HA-Puro

    Plasmid
    #193770
    Purpose
    Doxycyline-inducible (TetOn) human Geminin (C-terminal HA tag) with Dbox removed to prevent degradation by APC/C, expressed from all-in-one pCW vector (TetOn + rtTA)
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    GemininΔDbox-HA (GMNN Human)
    Use
    Lentiviral
    Tags
    HA
    Mutation
    Dbox removed (R23A, L26A). Codons switched at siR…
    Promoter
    TRE
    Available Since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193770-plasmid-map-sequence-id-380213

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  12. hGFAP-Cre

    Plasmid
    #40591
    Purpose
    Mouse astrocyte expression of Cre
    Depositor
    Albee Messing
    Article
    Zhuo et al Genesis. 2001 Oct;31(2):85-94.
    Insert
    Cre recombinase (GFAP Phage, Mouse, Human)
    Use
    Cre/Lox and Mouse Targeting
    Tags
    MP-1 fragment (mouse protamine 1 intron and polya…
    Expression
    Mammalian
    Mutation
    Construct contains a nuclear-targeted Cre recombi…
    Promoter
    hGFAP promoter
    Available Since
    Sept. 20, 2012
    Availability
    Academic Institutions and Nonprofits only
    40591-plasmid-map-sequence-id-302078

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  15. G protein alpha-s-YFP

    Plasmid
    #55781
    Purpose
    This G protein alpha-s construct contains internal insertions of YFP and the EE epitope.
    Depositor
    Catherine Berlot
    Article
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Insert
    alpha-s-EE YFP (Gnas Rat)
    Tags
    EYFP flanked by SGGGS on each side was inserted i…
    Expression
    Mammalian
    Mutation
    Met was substituted for Gln69 in EYFP (Clontech).…
    Promoter
    CMV
    Available Since
    Aug. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    55781-plasmid-map-sequence-id-119279

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  19. TFORF0361

    Plasmid
    #142255
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TCEB3 (ELOA Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142255-plasmid-map-sequence-id-395748

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  20. TFORF1445

    Plasmid
    #143276
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    GTF2H3 (GTF2H3 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    143276-plasmid-map-sequence-id-397436

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  21. TFORF1446

    Plasmid
    #143277
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    GTF2H3 (GTF2H3 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    143277-plasmid-map-sequence-id-396144

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  22. TFORF3186

    Plasmid
    #144662
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    GTF2H3 (GTF2H3 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144662-plasmid-map-sequence-id-350017

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  23. TFORF2693

    Plasmid
    #142359
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TEAD4 (TEAD4 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    142359-plasmid-map-sequence-id-397176

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  26. G protein alpha-s(alpha3beta5)

    Plasmid
    #55799
    Purpose
    This G protein alpha-s mutant exhibits increased affinity for and decreased ability to be activated by Gs-protein-coupled receptors as well as decreased ability to activate adenylyl cyclase.
    Depositor
    Catherine Berlot
    Article
    Berlot et al J Biol Chem. 2002 Jun 7;277(23):21080-5.
    Insert
    G protein alpha-s with alpha-i2 substitutions in alpha3/beta5 loop (Gnas Rat)
    Tags
    internal EE epitope (residues 189-194 in alpha-s …
    Expression
    Mammalian
    Mutation
    N271K, K274D, R280K, T284D, I285T in alpha-s sequ…
    Promoter
    CMV
    Available Since
    July 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    55799-plasmid-map-sequence-id-119340

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  29. G protein alpha-s(alpha3beta5/G226A)

    Plasmid
    #55798
    Purpose
    In addition to the mutations in alpha-s(alpha3beta5), this dominant negative G protein alpha-s mutant contains the G226A mutation, which impairs activating conformation changes in Switch II.
    Depositor
    Catherine Berlot
    Article
    Berlot et al J Biol Chem. 2002 Jun 7;277(23):21080-5.
    Insert
    alpha-s (alpha3beta5/G226A) (Gnas Rat)
    Tags
    internal EE epitope (residues 189-194 in alpha-s …
    Expression
    Mammalian
    Mutation
    N271K, K274D, R280K, T284D, I285T, G226A in alpha…
    Promoter
    CMV
    Available Since
    Aug. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    55798-plasmid-map-sequence-id-112177

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  39. pdCas9 (GB1079)

    Plasmid
    #75399
    Purpose
    Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part for C-terminal fusions
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10
    Insert
    Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available Since
    Aug. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75399-plasmid-map-sequence-id-151817

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Showing: 1961 - 2000 of 2485 results