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pMXs-hM₃O-IP

Plasmid

#46645

Depositor

Nobuaki Kikyo

Article

Hirai et al Stem Cells. 2011 Sep;29(9):1349-61. doi:

Insert

hM₃O (POU5F1 Human)

Use

Retroviral

Tags

IRES Puro and human MyoD (aa 1-62)

Expression

Mammalian

Mutation

contains a fragment of human MyoD (aa1-62) fused …

Available Since

July 22, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG-FLAG-puro-PTPIP51(1-470)

Plasmid

#170536

Purpose

Expresses PTPIP51(1-470) in human HeLa cells after transfection

Depositor

Byung Il Lee

Article

Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

Insert

Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)

Tags

FLAG tag

Expression

Mammalian

Promoter

CAG promoter

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only
pCfB2311 (SNR52p-gRNA.ADE2-SUP4t_natMX)

Plasmid

#83947

Purpose

gRNA cassette-carrying vector with natMX marker

Depositor

Irina Borodina

Article

Stovicek et al Metab Eng Commun. 2015 Mar 20;2:13-22. d

Insert

gRNA targeting ADE2 gene

Expression

Yeast

Available Since

Oct. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3-Myc3-Cul3 delta N41

Plasmid

#21590

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

Insert

cullin 3 (CUL3 Human)

Tags

myc3

Expression

Mammalian

Mutation

Residues 34-74 deleted from N terminus.

Available Since

July 1, 2009

Availability

Academic Institutions and Nonprofits only
APEX2-ChBP

Plasmid

#108878

Purpose

Expression of APEX2 fused to a nanobody against mCherry

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

APEX2-ChBP

Expression

Mammalian

Available Since

May 26, 2018

Availability

Academic Institutions and Nonprofits only

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pRS40B

Plasmid

#35478

Depositor

Steven Haase

Article

Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

Type

Empty backbone

Use

Yeast integrating vector

Available Since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pBK-CMV-hNHERF2

Plasmid

#47801

Purpose

Expresses human NHERF2 in mammalian cells

Depositor

Randy Hall

Article

Lau et al Biochemistry. 2001 Jul 24;40(29):8572-80

Insert

NHERF2 (NHERF2 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 3, 2013

Availability

Academic Institutions and Nonprofits only

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mMib1-FLAG

Plasmid

#37116

Depositor

Martin Dorf

Article

Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

Insert

Mib1(Mus musculus mindbomb homolog 1 (Drosophila) (Mib1)) (Mib1 Mouse)

Tags

3 x FLAG

Expression

Mammalian

Mutation

ACC Kozak sequence added before ATG annotated.

Promoter

CMV

Available Since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

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I-SceI pB 5UAS::ZfSyGCaMP2

Plasmid

#26125

Depositor

Leon Lagnado

Article

Dreosti et al Nat Methods. 2009 Dec . 6(12):883-9.

Insert

Synaptophysin GCaMP2 (sypb Zebrafish)

Use

Zebrafish expression

Tags

GCaMP2

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only

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pRSII316

Plasmid

#35455

Depositor

Steven Haase

Article

Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

Type

Empty backbone

Use

Yeast centromere vector

Promoter

None

Available Since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

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PL-5LTR-RGR(DMD#1)-AmCyan-A

Plasmid

#138482

Purpose

Expresses sgRNA targeting human DMD (Dystrophin) for packaging into NanoMEDIC particle.

Depositor

Akitsu Hotta

Article

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

Ribozyme-flanked gRNA and AmCyan

Use

CRISPR

Expression

Mammalian

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTet_NAD-Snifit-cyto

Plasmid

#113914

Purpose

Plasmid encoding the cytosolic localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cells

Depositor

Kai Johnsson

Article

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Insert

NAD-Snifit

Expression

Mammalian

Promoter

CMV TetO2

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pT2K-CAGGS-T2TP

Plasmid

#114725

Purpose

expression in mammalian cells

Depositor

Martine Roussel

Article

Kawauchi et al Oncogene. 2017 Sep 14;36(37):5231-5242.

Insert

Tol2 transposase

Expression

Mammalian

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP Tubulin K40Q

Plasmid

#105302

Purpose

To study effects of acetyl-mimetic (hyper-acetylated) tubulin

Depositor

Kenneth Yamada

Article

Joo et al Nat Commun. 2014 Mar 25;5:3510. doi: 10.

Insert

alpha tubulin (TUBA1B Human)

Tags

EGFP

Expression

Mammalian

Mutation

K40Q

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Hygro(+)-PSTCD-cGAS

Plasmid

#102605

Purpose

Mammalian expression of cGAS fused to PSTCD tag

Depositor

Nicolas Manel

Article

Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

Insert

cGAS (CGAS Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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HaloTag-G protein beta-1

Plasmid

#67016

Purpose

G protein beta-1 tagged with HaloTag on amino terminus

Depositor

Catherine Berlot

Insert

HaloTag-G protein beta-1 (GNB1 Human)

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pMAX deltaN-ERG

Plasmid

#29447

Depositor

Valeri Vasioukhin

Article

Klezovitch et al Proc Natl Acad Sci U S A. 2008 Feb 12. 1

Insert

ERG (ERG Human)

Expression

Mammalian

Mutation

Deletion of first 33 aa of ERG

Available Since

March 31, 2011

Availability

Academic Institutions and Nonprofits only
pAD-CMV-STAT5B-CMV-GFP

Plasmid

#83257

Purpose

Adenoviral expression of STAT5B with co-expression of GFP

Depositor

Andrew Brooks

Article

Legrand et al J Invest Dermatol. 2016 Sep;136(9):1781-

Inserts

Stat5b (Stat5b Mouse)
eGFP

Use

Adenoviral

Promoter

CMV

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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v3em-Cterm-PE6c-dualU6-Rosa26-twinPE-attB

Plasmid

#207861

Purpose

AAV genome encoding C-terminal PE6c and U6 expression cassettes for Rosa26 twinPE pegRNA pairs

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

NpuC-Cterm-PE6c-dualU6-Rosa26-twinPE-attB

Use

AAV

Mutation

See Manuscript

Promoter

Cbh

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMVA2RBS035

Plasmid

#121051

Purpose

Expresses recombinant MVA pathway in Escherichia coli

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

Inserts

mvaE
mvaS
mvaK1
mvaK2
mvaD
idi

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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