We narrowed to 10,361 results for: POR
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Plasmid#131849PurposeExpresses the N-terminal nuclear export signal of HcRed-NPMMLF1 in mammalian cells through transient transfectionDepositorInsertNPM1 (NPM1 Human)
TagsHcRedExpressionMammalianMutationThis is a control deletion contruct of the cytop…Available SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-uTEV1-MAVS-mTA2
Plasmid#171000PurposeHiLITR protease with MAVS [R537A, R538A] C-terminal tmd targeting information (Mitochondria and ER insertion)DepositorInsertEGFP-uTEV1-MAVS(tmd)[R537A, R538A]
UseLentiviral and Synthetic BiologyExpressionMammalianMutationMAVS(tmd)[R537A, R538A]PromoterpTRE-tight (TetOn)Available SinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-uTEV1-MAVS-mTA5
Plasmid#171003PurposeHiLITR protease with MAVS [Y536delinsFIVLI, R537A] C-terminal tmd targeting information (Mitochondria and ER insertion)DepositorInsertEGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI, R537A]
UseLentiviral and Synthetic BiologyExpressionMammalianMutationMAVS(tmd)[Y536delinsFIVLI, R537A]PromoterpTRE-tight (TetOn)Available SinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
PMP34-hLOV-TEVcs-GAL4bd-VP64-V5
Plasmid#170997PurposeHiLITR transcription factor with full-length PMP34 targeting sequence (Peroxisome)DepositorInsertPMP34(FL)-NNES-hLOV-TEVcs(ENLYFQ-M)-GAL4bd-VP64-V5 (SLC25A17 Synthetic)
UseLentiviral and Synthetic BiologyTagsV5ExpressionMammalianPromoterEf1-alphaAvailable SinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
prCOrev
Plasmid#153961PurposeUsed to detect crossover-type DNA recombination events elicited by iso-positional nicking of two homologous DNA fragments (recombination analogous to Holliday's model)DepositorInsert5' and 3' EGFP fragments sharing a 386-bp sequence, separated by a NeoR gene cassette and arranged in the opposite direction
UseRetroviral; A reporter plasmid detecting dna reco…TagsNLS-ZFExpressionMammalianMutationNo mutationPromoterLTRAvailable SinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
prCO
Plasmid#153960PurposeUsed to detect crossover-type DNA recombination events elicited by iso-positional nicking of two homologous DNA fragments (recombination analogous to Holliday's model)DepositorInsert5' and 3' EGFP fragments sharing a 386-bp sequence, separated by a NeoR gene cassette and arranged in the same direction
UseRetroviral; A reporter plasmid detecting dna reco…TagsNLS-ZFExpressionMammalianMutationNo mutationPromoterLTRAvailable SinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
SMT215
Plasmid#134818PurposeSMT205 with DHFR-FLAG-tag, western blotDepositorInsertsDihydrofolate reductase
Thymidylate synthase
TagsFLAGExpressionBacterialMutationSilent mutations to eliminate internal restrictio…PromoterT7 and TETAvailable SinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
SMT201
Plasmid#134816PurposeSMT101 with TET promoter for TYMS, in vivo assaysDepositorInsertsDihydrofolate reductase
Thymidylate synthase
ExpressionBacterialMutationSilent mutations to eliminate internal restrictio…PromoterT7 and TETAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
N229A Lo-MetQ
Plasmid#118268PurposeN229A Mutation in Methionine Binding Protein (MetQ) used to solve crystal structureDepositorInsertMetQ (N229A) (metQ E. coli)
Tags10x His Tag and Enterokinase Cleavage SiteExpressionBacterialMutationChanged Asparagine (Asn) 229 to Alanine (Ala)PromoterT7 PromoterAvailable SinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAL829
Plasmid#119697Purposeprecursor slow-folding maltodextrin binding protein, MBP or malE, with mutations Y283D, G289C and V347CDepositorInsertslow-folding maltose ABC transporter periplasmic binding protein, malE, with mutations Y283D, G289C and V347C
TagsnoneExpressionBacterialMutationmutations Y283D, G289C and V347CPromoterT5Available SinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-1xTS
Plasmid#109418PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to one tyrosine sulfation (TS) motif. VHH-1xTS also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
TagsBAP, HA, His6, T7, and Tyrosine sulfation (TS) se…ExpressionBacterialPromoterT7Available SinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b+3(R112C/T180C)C
Plasmid#108193PurposeExpresses PCNA3(R112C/T180C)-P450cam fusion protein in E. coliDepositorInsertPCNA3(R112C/T180C)-P450cam fusion protein
TagsHis6 tagExpressionBacterialMutationR112C/T180C(PCNA3)PromoterT7 promoterAvailable SinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b+2(L171C)X
Plasmid#108192PurposeExpresses PdR-PCNA2(L171C)-PdX fusion protein in E. coliDepositorInsertPCNA2(L171C)-PdX fusion protein
TagsHis6 tagExpressionBacterialMutationL171C (PCNA2); C73S/C85S (PdX)PromoterT7 promoterAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b+R2(L171C)X
Plasmid#108191PurposeExpresses PdR-PCNA2(L171C)-PdX fusion protein in E. coliDepositorInsertPdR-PCNA2(L171C)-PdX fusion protein
TagsHis6 tagExpressionBacterialMutationL171C (PCNA2); C73S/C85S (PdX)PromoterT7 promoterAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b+1(G108C)X
Plasmid#108189PurposeExpresses PCNA1(G108C)-PdX fusion protein in E. coliDepositorInsertPCNA1(G108C)-PdX fusion protein
TagsHis6 tagExpressionBacterialMutationG108C (PCNA1); C73S/C85S (PdX)PromoterT7 promoterAvailable SinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLuc-OptoJNKi
Plasmid#89744PurposeExpression of light-regulated JNK inhibitor, firefly luciferase-fused, wild-type photosensor, inhibitor JIP11DepositorInsertOptoJNKi
UseLuciferaseTagsFirefly luciferaseExpressionMammalianPromoterCMVAvailable SinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-RP2-C86Y,P95L
Plasmid#86074PurposeA bacterial expression plasmid encoding ciliary localization effected RP2 (RP2-C86Y,P95L) with N-terminus GST-tag.DepositorInsertRP2 (RP2 Human)
TagsGSTExpressionBacterialMutationChanged cysteine-86 and proline-95 to tyrosine an…PromotertacAvailable SinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
CFFΔC-EGFP
Plasmid#86059PurposeA mammalian expression plasmid encoding fibrocystin mimentic clone CFFΔC (CD8a luminal domain, fibrocystin transmembrane and ciliary targeting signal) with C-terminus EGFP.DepositorInsertFibrocystin (Pkhd1 Mouse)
TagsGFPExpressionMammalianMutationcontains CD8a luminal and transmembrane domains a…PromoterCMVAvailable SinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPD2cX
Plasmid#86968PurposeExpresses PCNA2 variant (L171C) fused phosphite dehydrogenase and putidaredoxin in E. coliDepositorInsertPCNA2 variant (L171C) fused to phosphite dehydrogenase and putidaredoxin
TagsHis6 tagExpressionBacterialMutationE175A/A176R in PTDH, C73S/C85S in PdX, L171C in P…PromoterT7 promoterAvailable SinceMarch 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNIA-CEN-FLAG-LINXa4-Bre1
Plasmid#73617PurposeControl of the E3 ligase Bre1 with LINXa in yeast.DepositorInsertLINXa4-Bre1dNLS
UseSynthetic BiologyTagsFLAGExpressionYeastMutationBre1 point mutations K8A, K9A and K11APromoterADH1Available SinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only