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MTK2_018

Plasmid

#123710

Purpose

Encodes truncated pCMV as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pCMV-C

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pmECFPC1/GgVcl 1-1066 (CFP at N term)

Plasmid

#46281

Depositor

Susan Craig

Article

Chen et al J Cell Biol. 2005 May 9;169(3):459-70.

Insert

Vcl 1-1066 (VCL Chicken)

Tags

ECFP

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2013

Availability

Academic Institutions and Nonprofits only

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pPK720

Plasmid

#38147

Depositor

Patricia Kuwabara

Article

Soloviev et al Dev Biol. 2011 Mar 15;351(2):242-53. Epu

Insert

dpy-7p::ptc-3::gfp (ptc-3 )

Tags

EGFP

Expression

Worm

Promoter

dpy-7

Available Since

Oct. 25, 2012

Availability

Academic Institutions and Nonprofits only

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p5Ecldn5bE1

Plasmid

#90113

Purpose

5' Gateway entry clone containing endothelial specific enhancer sequence of cldn5b

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

cldn5bE1 (cldn5b Zebrafish)

Expression

Bacterial

Available Since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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p3994 pGEM HHR23A

Plasmid

#10863

Depositor

Peter Howley

Article

Kumar et al J Biol Chem 1997 May 23;272(21):13548-54

Insert

HHR23A (RAD23A Human)

Expression

Bacterial

Available Since

Oct. 26, 2005

Availability

Academic Institutions and Nonprofits only
pRC02

Plasmid

#114681

Purpose

sfGFP.Strand10-Haloenzyme

Depositor

Matthew Good

Article

Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

Insert

sfGFP.Strand10-Haloenzyme

Tags

His10

Expression

Bacterial

Promoter

T7

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA4.1/HisMax-Syx1

Plasmid

#35708

Depositor

Arie Horowitz

Article

Liu et al Mol Biol Cell. 2006 Apr;17(4):1880-7. Ep

Insert

Syx1 (Plekhg5 Mouse)

Tags

6x HIS and Xpress

Expression

Mammalian

Mutation

R205K

Promoter

CMV

Available Since

June 22, 2012

Availability

Academic Institutions and Nonprofits only
2xDHFR-myc

Plasmid

#20215

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

2xDHFR (folA E. coli)

Tags

myc

Expression

Mammalian

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pBS C-ret

Plasmid

#119900

Purpose

Mouse Ret In situ hybridization probe

Depositor

David Ginty, Wenqin Luo

Article

Luo et al Neuron. 2009 Dec 24;64(6):841-56. doi: 1

Insert

ret (Ret Mouse)

Use

In situ hybridization probe

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pPK348

Plasmid

#38145

Depositor

Patricia Kuwabara

Article

Soloviev et al Dev Biol. 2011 Mar 15;351(2):242-53. Epu

Insert

ptc-3::GFP (ptc-3 Nematode)

Expression

Worm

Promoter

ptc-3

Available Since

Oct. 25, 2012

Availability

Academic Institutions and Nonprofits only

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pIS1-miR-126 site

Plasmid

#25026

Depositor

Bob Weinberg

Article

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Insert

synthetic miR-126 site

Use

Luciferase

Expression

Mammalian

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
pSicoR-TTF1i2

Plasmid

#59621

Purpose

Expression of shRNA against human TTF1

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

TTF1 (TTF1 Human)

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
pQCXIP-Laforin Myc R171H

Plasmid

#84671

Purpose

mammalian expression and/or retrovirus preparation

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Promoter

5'LTR

Available Since

Nov. 24, 2016

Availability

Academic Institutions and Nonprofits only
pPD_Pinx-1::G-CaMP3

Plasmid

#117422

Purpose

For Calcium imaging, expression of G-CaMP3 under the expression of a Pinx-1 promoter (AIB interneurons)

Depositor

Shohei Mitani

Article

Hori et al PLoS Genet. 2018 Jun 25;14(6):e1007477.

Insert

G-CaMP3

Expression

Worm

Promoter

inx-1

Available Since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

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p067 pCS mTRPS1 P4

Plasmid

#11039

Depositor

Ramesh Shivdasani

Article

Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

Insert

TRPS1 P4 (Trps1 Mouse)

Use

Xenopus, mammalian, avian, and zebrafish

Mutation

Truncation at aa1121 to mimic frameshift at codon…

Available Since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only
pESC-LEU2-RAD30cmyc

Plasmid

#60821

Purpose

to overexpress yeast polymerase eta in yeast

Depositor

Thomas Kunkel, Youri Pavlov

Article

Pavlov et al Mutat Res. 2001 Jul 1;478(1-2):129-39.

Insert

RAD30 (RAD30 Budding Yeast)

Tags

c-myc

Expression

Yeast

Promoter

GAL1

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
pRS424-TUB2 (1-428)

Plasmid

#60395

Purpose

Expression of yeast beta tubulin (TUB2) - without Cterminal tail using GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Expression

Yeast

Mutation

deleted 429 - end

Promoter

GAL

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
T7-AglC-His6

Plasmid

#89726

Purpose

Expresses AglC from M. voltae with an N-terminal T7 tag and a C-terminal His6 tag. Plasmid has codons optimized for E. coli expression.

Depositor

Barbara Imperiali

Article

Zamora et al Methods Enzymol. 2017;597:145-186. doi:

Insert

AglC

Tags

His6 and T7

Expression

Bacterial

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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T7-AglK-His

Plasmid

#89714

Purpose

Expresses AglK from M. voltae with an N-terminal T7 tag and a C-terminal His6 tag. Plasmid has codons optimized for E. coli expression.

Depositor

Barbara Imperiali

Article

Zamora et al Methods Enzymol. 2017;597:145-186. doi:

Insert

AglK

Tags

His6 and T7

Expression

Bacterial

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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His8-TEV-PseC

Plasmid

#89724

Purpose

Expresses PseC from C. jejuni with an N-terminal octaHis tag and TEV cleavage site

Depositor

Barbara Imperiali

Article

Zamora et al Methods Enzymol. 2017;597:145-186. doi:

Insert

PseC (pseC Campylobacter jejuni)

Tags

His8 and TEV

Expression

Bacterial

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only