We narrowed to 25,212 results for: lars;

1. pFUGW-hGtACR2-EYFP

   Plasmid

   #67877

   Purpose

   Mammalian expression construct of human codon-adapted anion channelrhodopsin 2 from Guillardia theta fused in frame to EYFP via a NotI site in the pFUGW vector backbone

   Depositor

   John Spudich

   Article

   Govorunova et al Science. 2015 Jun 25. pii: aaa7484.

   Insert

   hGtACR2
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   UbC

   Available since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. CAG sHRPa-NRX

   Plasmid

   #73143

   Purpose

   Large fragment of split HRP fused to the extracellular terminus of neurexin3B. CAG promoter.

   Depositor

   Alice Ting

   Article

   Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

   Insert

   sHRPa-NRX
   

   Use

   Tags

   2x HA tag and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCIS GEM-CEPIA1er

   Plasmid

   #58217

   Purpose

   Ratiometric (Blue to Green) fluorescent indicator for calcium signaling in the endoplasmic reticulum

   Depositor

   Masamitsu Iino

   Article

   Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

   Insert

   GEM-CEPIA1er
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   GEM-GECO1 E298D F359W D400E E407D

   Promoter

   CAG

   Available since

   July 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS426 Gal TDP43

   Plasmid

   #27466

   Depositor

   Aaron Gitler

   Article

   Johnson et al Proc Natl Acad Sci U S A. 2008 Apr 29. 1

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 31, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pCLX-CHOP-dGFP

   Plasmid

   #71299

   Purpose

   Lentiviral plasmid to express destabilized GFP under the control of the ATF-responsive CHOP promoter

   Depositor

   Patrick Salmon

   Article

   Boitard et al Cell Rep. 2015 Mar 3;10(8):1349-61. doi:

   Insert

   dGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CHOP (ATF-responsive)

   Available since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMLS-SV40-EGFP

   Plasmid

   #46919

   Purpose

   Target EGFP gene that is stably integrated into HEK293 genome

   Depositor

   Stanley Qi, Jonathan Weissman

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Insert

   GFP
   

   Use

   CRISPR and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-ConVERGD-ConFonvCon-eGFP-W3SL

   Plasmid

   #218752

   Purpose

   Provides AND intersectional (Cre+Flp+vCre) expression of eGFP

   Depositor

   Lindsay Schwarz

   Article

   Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

   Insert

   eGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. NbV5-HALOTAG

   Plasmid

   #201482

   Purpose

   Indirect detection of V5-tagged protein using the HaloTag protein labeling technology.

   Depositor

   Patrick Giguère

   Article

   Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

   Insert

   NbV5-HALOTAG
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-cMSP1E3D1

   Plasmid

   #199772

   Purpose

   Expresses split-intein fused MSP1E3D1 for circularization during expression (external His-Tag)

   Depositor

   Franz Hagn

   Article

   Daniilidis et al J Mol Biol. 2022 Dec 30;434(24):167861.

   Insert

   cMSP1E3D1
   

   Use

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   Extended cMSP1D1; external His-Tags flanking the …

   Promoter

   Available since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-hSyn-ConVERGD-TVA(mChr)-W3SL

    Plasmid

    #218754

    Purpose

    Provides AND intersectional (Cre+Flp) expression of TVA-mCherry

    Depositor

    Lindsay Schwarz

    Article

    Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

    Insert

    TVA-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-hSyn-ConVERGD-ConFonvConNon-eGFP-W3SL

    Plasmid

    #218753

    Purpose

    Provides AND intersectional (Cre+Flp+vCre+Vika) expression of eGFP

    Depositor

    Lindsay Schwarz

    Article

    Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. FRB_SCF2_LD6_CTEV

    Plasmid

    #58879

    Purpose

    MESA split-TEV protease chain with Rapamycin FRB ectodomain 2 aa scaffold, 6 aa linker domain, C-terminal TEV protease

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    MESA split protease chain with FRB rapamycin-binding ectodomain and C terminal fragment of TEV protease
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pNubWtXgate

    Plasmid

    #105079

    Purpose

    Yeast mating-based Split Ubiquitin Assay, positive control

    Depositor

    Wolf Frommer

    Article

    Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

    Type

    Empty backbone

    Use

    Tags

    NubI

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FKBP_SCF2_LD6_NTEV

    Plasmid

    #58878

    Purpose

    MESA split-TEV protease chain with Rapamycin FKBP ectodomain, 2 aa scaffold, 6 aa linker domain, N-terminal TEV protease

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    MESA split protease chain with FKBP rapamycin-binding ectodomain and N terminal fragment of TEV protease
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. E1b-GFP-Tol2-Gateway

    Plasmid

    #37846

    Depositor

    Nadav Ahituv

    Article

    Birnbaum et al Genome Res. 2012 Jun;22(6):1059-68. Epub

    Type

    Empty backbone

    Use

    Zebrafish enhancer assay vector

    Tags

    Expression

    Mutation

    Promoter

    E1b

    Available since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDONR TDP43 WT YFP

    Plasmid

    #27470

    Depositor

    Aaron Gitler

    Article

    Johnson et al Proc Natl Acad Sci U S A. 2008 Apr 29. 1

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFUGW-hGtACR1-EYFP

    Plasmid

    #67795

    Purpose

    Mammalian expression construct of human codon-adapted anion channelrhodopsin 1 from Guillardia theta fused in frame to EYFP via a NotI site in the pFUGW vector backbone

    Depositor

    John Spudich

    Article

    Govorunova et al Science. 2015 Jun 25. pii: aaa7484.

    Insert

    hGtACR1
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-hSyn-ConVERGD-N2cG-WPRE

    Plasmid

    #218757

    Purpose

    Provides AND intersectional (Cre+Flp) expression of N2C glycoprotein

    Depositor

    Lindsay Schwarz

    Article

    Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

    Insert

    N2cG
    

    Use

    AAV

    Tags

    Expression

    Mutation

    See Depositor Comments

    Promoter

    hSyn

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-hSyn-eNpHR 3.0-EYFP

    Plasmid

    #26775

    Purpose

    3rd gen lentiviral expression of eNpHR 3.0 fused to EYFP driven by human Synapsin I promoter for optogenetic inhibition

    Depositor

    Karl Deisseroth

    Article

    Gradinaru et al Cell. 2010 Apr 2. 141(1):154-65.

    Insert

    eNpHR 3.0
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Trafficking Signal (TS) ER Export Signal

    Promoter

    hSyn

    Available since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 3XHA-PP2A-Ba

    Plasmid

    #195229

    Purpose

    Retroviral vector containing HA tagged PP2A

    Depositor

    David Bryant, Keith Mostov

    Article

    Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

    Insert

    PPP2R2A (PPP2R2A Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLBM0010

    Plasmid

    #172816

    Purpose

    Level 2 module containing HSP21-GFP

    Depositor

    Benjamin Field

    Article

    Velay et al Plant Methods. 2022 May 26;18(1):69. doi

    Insert

    [35S-P19][35S-CTPSSU-mHSP21-GFP]
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET28a-cMSP1D1

    Plasmid

    #199768

    Purpose

    Expresses split-intein fused MSP1D1 for circularization during expression (external His-Tag)

    Depositor

    Franz Hagn

    Article

    Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

    Insert

    cMSP1D1
    

    Use

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    external His-Tags flanking the C and N termini

    Promoter

    Available since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. FKBP_LD 0_TEV

    Plasmid

    #58877

    Purpose

    MESA protease chain with Rapamycin FKBP ectodomain and no linker domain

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    MESA protease chain with FKBP rapamycin-binding ectodomain, TEV protease and mCherry fusion
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pIS002

    Plasmid

    #194013

    Purpose

    This plasmid is designed for use in a procedure to synthesize single-stranded DNA containing only the insert.

    Depositor

    Timothy Whitehead

    Article

    Strawn et al Biotechnol Bioeng. 2023 Oct;120(10):3057

    Insert

    C16 Ex3 XTR f1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-hSyn-ConVERGD-ConFoff-TVA(mChr)-W3SL

    Plasmid

    #218756

    Purpose

    Provides NOT intersectional (Cre NOT Flp) expression of TVA-mCherry

    Depositor

    Lindsay Schwarz

    Article

    Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

    Insert

    TVA-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-hSyn-ConVERGD-CoffFon-TVA(mChr)-W3SL

    Plasmid

    #218755

    Purpose

    Provides NOT intersectional (Flp NOT Cre) expression of TVA-mCherry

    Depositor

    Lindsay Schwarz

    Article

    Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

    Insert

    TVA-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET28a-cMSPdH5

    Plasmid

    #199771

    Purpose

    Expresses split-intein fused MSPdH5 for circularization during expression (external His-Tag)

    Depositor

    Franz Hagn

    Article

    Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

    Insert

    cMSPdH5
    

    Use

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    external His-Tags flanking the C and N termini

    Promoter

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CAG-loxP-3xpA-loxP-H2B-sfGFP

    Plasmid

    #136064

    Purpose

    H2B-sfGFP cre reporter

    Depositor

    Mario Capecchi

    Article

    Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

    Insert

    HIST1H2BB (H2BC3 Human)
    

    Use

    Cre/Lox

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSplit-CO-hDib1

    Plasmid

    #58788

    Purpose

    Express LucC-hDib1 fusion protein in mammalian cells

    Depositor

    Hiroyoshi Ariga

    Article

    Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

    Insert

    hDib1 (TXNL4A Human)
    

    Use

    Tags

    Flag and Luciferase (394-550aa)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. mTFP1-C-Src-7

    Plasmid

    #55472

    Purpose

    Localization: Membrane/Vesicles, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    C-Src
    

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge