We narrowed to 1,917 results for: FRT

1. Syn-FRT-MCS-WPRE-SV40 - GCH9

   Plasmid

   #230819

   Purpose

   AAV cloning vector for Flpo-dependent expression under the Syn promoter.

   Depositor

   Kimberly Ritola

   Type

   Empty backbone

   Use

   AAV; Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFRT-PonA-BI-Gl-GFP-PTC-mCherry-WT (5FP-WP)

   Plasmid

   #189795

   Purpose

   Express GFP/mCherry linked wild-type and PTC-containing beta-globin bi-directionally under ponasteron A inducible promoter in mammalian cells

   Depositor

   Robert Singer

   Article

   Sato et al Nat Commun. 2021 Dec 10;12(1):7203. doi:

   Insert

   beta-globin (HBB Mouse, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ponasterone A inducible

   Available Since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Frt-Atg-Hygro-CMV-green (mWasabi)-PA-F3

   Plasmid

   #67276

   Purpose

   RMCE donor plasmid for FlpO. the marker gene is green fluorescent (mWasabi) with PA

   Depositor

   Jannik Elverløv-Jakobsen

   Article

   Callesen et al PLoS One. 2016 Aug 19;11(8):e0161471. do

   Inserts

   GFP (mWasabi)
   hygro
   

   Use

   Tags

   F3 and Frt

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFRT-TO-RPB1-His K1278R CRres si2,4R

   Plasmid

   #139406

   Purpose

   Dox-inducible, K1278R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

   Depositor

   Jesper Svejstrup

   Article

   Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

   Insert

   RPB1 (POLR2A Human)
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   K1278R

   Promoter

   Available Since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-CMV-FRT-myrSNAP-2A-H2BeGFP-WPRE

   Plasmid

   #102986

   Purpose

   AAV expressing membrane-targeted SNAP protein and nuclear-targeted eGFP after FLP-mediated recombination

   Depositor

   Marco Tripodi

   Article

   Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

   Inserts

   membrane-targeted SNAP protein after FLP-mediated recombination
   Nuclear-targeted eGFP after FLP-mediated recombination
   

   Use

   AAV; Adeno associated viral vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA5 FRT TO myc Separase Non-Cleavable

   Plasmid

   #59826

   Purpose

   Allows the integration of myc Separase Non-Cleavable in the genome and Tet-inducible expression.

   Depositor

   Stephen Taylor

   Article

   Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

   Insert

   Separase (ESPL1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Non-Cleavable (E1483R, R1486E, E1503R, R1506E, E1…

   Promoter

   hybrid human cytomegalovirus (CMV)/TetO2 promoter

   Available Since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA5FRT/Flag-hINO80 ΔN ΔHSA (520-1556)

   Plasmid

   #29446

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

   Insert

   INO80 (INO80 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   INO80 520-1556 (a.a.)

   Promoter

   Available Since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA5FRT/FLAG-Ino80 ΔNC (267-1261)

   Plasmid

   #29439

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

   Insert

   INO80 (INO80 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   INO80 267-1261 (a.a.) with Q1116K mutation

   Promoter

   Available Since

   Aug. 1, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA5FRT/FLAG-Ino80 ΔNC-EQ (267-1261)

   Plasmid

   #29437

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

   Insert

   INO80 (INO80 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   INO80 267-1261 (a.a.) E653Q

   Promoter

   Available Since

   July 1, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pDEST-FRT/T0-GFP-BRCA1 1364-1437

    Plasmid

    #71119

    Purpose

    Mammalian expression of GFP-BRCA1 1364-1437

    Depositor

    Daniel Durocher

    Article

    Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains amino acids 1364-1437 of BRCA1

    Promoter

    CMV/TO

    Available Since

    Dec. 9, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pEF5/FRT/DEST-3xHA/mGli3/Flag P5-6A

    Plasmid

    #51249

    Purpose

    Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 980,1006 mutated to Ala

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli3 (Gli3 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV and Flag

    Expression

    Mammalian

    Mutation

    amino acids 980,1006 mutated to Ala

    Promoter

    EF1a

    Available Since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pFRT-TO-RPB1-His CRres K619,627,642R si2,4R

    Plasmid

    #139409

    Purpose

    Dox-inducible, K619, 627, 642R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

    Depositor

    Jesper Svejstrup

    Article

    Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

    Insert

    RPB1 (POLR2A Human)
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    K619, 627, 642R

    Promoter

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFRT-TO-RPB1-His K751,758,767R CRres si2,4R

    Plasmid

    #139408

    Purpose

    Dox-inducible, K751, 758, 767R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

    Depositor

    Jesper Svejstrup

    Article

    Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

    Insert

    RPB1 (POLR2A Human)
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    K751, 758, 767R

    Promoter

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFRT-TO-RPB1-His K710,719R CRres si2,4R

    Plasmid

    #139407

    Purpose

    Dox-inducible, K710, 719R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

    Depositor

    Jesper Svejstrup

    Article

    Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

    Insert

    RPB1 (POLR2A Human)
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    K710,719R

    Promoter

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA5 FRT TO myc Separase S1226A/ C2029A

    Plasmid

    #59823

    Purpose

    Allows the integration of myc Separase S1226A/ C2029A in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Holland et al J Cell Sci. 2006 Aug 15;119(Pt 16):3325-

    Insert

    Separase (ESPL1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    S1226A/ C2029A

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEF5/FRT/DEST-3xHA/mGli3/Flag P1-4A

    Plasmid

    #51247

    Purpose

    Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907 mutated to Ala

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli3 (Gli3 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV and Flag

    Expression

    Mammalian

    Mutation

    amino acids 849,865,877,907 mutated to Ala

    Promoter

    EF1a

    Available Since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA5FRT/Flag-hINO80 HSA (212-526)

    Plasmid

    #29443

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

    Insert

    INO80 (INO80 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    INO80 212-526 (a.a.)

    Promoter

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

18. Triple ORF FRT ifgMosaic Donor (IG135)

    Plasmid

    #99626

    Purpose

    To clone the 3 ORFs for FlpO dependent generation of ifgMosaics

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    PGK-Neo
    

    Use

    Cre/Lox, Mouse Targeting, and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA5-FRT/TO-neo-C11orf51-3xFlag (2765)

    Plasmid

    #39859

    Depositor

    Jonathon Pines

    Article

    Mansfeld et al Nat Cell Biol. 2011 Sep 18;13(10):1234-4

    Insert

    C11orf51 (ANAPC15 Human)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pBW768_pCAG-PV1-FRT-lox2272-BFP-BGHpA

    Plasmid

    #87574

    Purpose

    PV1 for 2-input, 4-output decoder circuit

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    mtagBFP
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge