We narrowed to 6,231 results for: nls

1. p1194-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIA4_FLAG_NLS

   Plasmid

   #129532

   Purpose

   Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIA4

   Depositor

   Erik Sontheimer

   Article

   Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

   Inserts

   codon-optimized AcrIIA4
   sgRNA_Rosa26
   

   Use

   AAV

   Tags

   FLAG/NLS

   Expression

   Mutation

   Promoter

   U6 and cytomegalovirus-enhancer chicken β-actin p…

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-GFAP-2xNLS-mTurquoise2

   Plasmid

   #104053

   Purpose

   An AAV genome encoding expression of the nuclear localized fluorescent protein mTurquoise2 from the GFAP promoter

   Depositor

   Viviana Gradinaru

   Article

   Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

   Insert

   2xNLS-mTurquoise2
   

   Use

   AAV

   Tags

   NLS

   Expression

   Mutation

   Promoter

   GFAP

   Available Since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5_NLS-Halo-eGFP-BioID

   Plasmid

   #218448

   Purpose

   expresses the NLS-Halo-eGFP-BioID fusion protein via stable Flp-In integration

   Depositor

   Thorsten Stafforst

   Article

   Hanswillemenke et al Nat Chem Biol. 2024 Jan 17. doi: 10.1038

   Insert

   NLS-Halo-eGFP-BirA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR_TRE3GS-->GssGFP-P2A-NLSIFP

   Plasmid

   #162235

   Purpose

   Lentiviral vector for the expression of TRE3GS-->GssGFP-P2A-NLSIFP in mammalian cells

   Depositor

   Wendell Lim

   Article

   Toda et al Science. 2020 Oct 16;370(6514):327-331.

   Insert

   GFP - IFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQCXIP-NLS-Vx3-mEGFP

   Plasmid

   #35529

   Depositor

   Robert Cohen

   Article

   Sims et al Nat Methods. 2012 Feb 5. doi: 10.1038/nm

   Insert

   NLS-Vx3
   

   Use

   Retroviral

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. YIPlac204TC-NLS-DsRed-Express2

   Plasmid

   #25443

   Depositor

   Benjamin Glick

   Article

   Strack et al Nat Methods. 2008 Nov . 5(11):955-7.

   Insert

   NLS-DsRed-Express2
   

   Use

   Tags

   NLS

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   July 14, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTS2394-Tier1-PhCMV-3xNLS_mTagBFP2

   Plasmid

   #169531

   Purpose

   Tier-1 vector encoding PhCMV-driven blue fluorescent protein mTagBFP2 with nuclear localization (PhCMV-3xNLS-mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven nucleus-localized monomeric Tag blue fluorescent protein 2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-NPM NESM-NLS1D

   Plasmid

   #13288

   Depositor

   Xin Wang

   Article

   Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

   Insert

   NPM (NPM1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   L100A, L102A, deletion of aa134-aa142

   Promoter

   Available Since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

9. mTubulin NLS-mCherry-LEXY

   Plasmid

   #182597

   Purpose

   Drosophila integration plasmid expressing NLS-mCherry-LEXY fusion protein under the maternal tubulin promoter.

   Depositor

   Jared Toettcher

   Article

   Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

   Insert

   mCherry
   

   Use

   Tags

   LEXY and NLS

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX-4T1 NLS-GFP

    Plasmid

    #129441

    Purpose

    bacterial expression of GFP

    Depositor

    Oskar Laur

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-opCas12a-Ultra-6xNLS

    Plasmid

    #209024

    Purpose

    Lentiviral vector expressing human codon-optimized opCas12a-Ultra with 6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    opCas12a-Ultra-6xNLS-Flag-Myc-NeoR
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG and MYC

    Expression

    Mammalian

    Mutation

    E174R/M537R/S542R/F870L

    Promoter

    EF-1a

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGTag-NLS-TagRFP-B-actin

    Plasmid

    #117806

    Purpose

    Donor for precise CRISPR directed genomic integration using the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    TagRFP
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFUW-TetO-DsRed-cNLS

    Plasmid

    #138533

    Purpose

    Lentiviral inducible expression of DsRed-Express2 with nuclear localization signal

    Depositor

    Ana Teixeira, Christopher Grigsby

    Article

    Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

    Insert

    DsRed-Express2
    

    Use

    Lentiviral

    Tags

    3x SV40 cNLS

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-CMV

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-TetR-NLS-VP16

    Plasmid

    #103834

    Purpose

    Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited tetO arrays

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    EGFP-TetR-NLS-VP16 (tetR )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV2

    Available Since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. 416Gal-SV40NLS-FUS-YFP

    Plasmid

    #29595

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    NLS-FUS (FUS Human)
    

    Use

    Tags

    SV40 NLS and YFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pGTag-NLS-eGFP-B-actin

    Plasmid

    #117812

    Purpose

    Donor for precise CRISPR directed genomic integration using the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMTB2-NLS-BirA-2A-mCherry_Ras

    Plasmid

    #80067

    Purpose

    Beta-actin2 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Beta-actin2

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. NLS-FUS-Citrine-PixE

    Plasmid

    #111506

    Purpose

    For the nuclear localization of the PixELL system

    Depositor

    Jared Toettcher

    Article

    Dine et al Cell Syst. 2018 May 24. pii: S2405-4712(

    Insert

    PixE (slr1693 )
    

    Use

    Lentiviral

    Tags

    Citrine, FUS, and NLS

    Expression

    Mutation

    Promoter

    SFFV

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. PET-B-HypaSpCas9-NLS-6xHis

    Plasmid

    #207386

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HypaSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N692A, M694A, Q695A, H698A, amino acids 1005-1013…

    Promoter

    T7

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-mCherry-TSC2(AA,3Q)-NLS

    Plasmid

    #192445

    Purpose

    Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A, K1614Q, K1615Q, R1616Q mutations to disrupt Akt phosphorylation and GAP activity; targeted to the nucleus.

    Depositor

    Jin Zhang

    Article

    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

    Insert

    mCherry-TSC2(S939A,T1439A,K1614Q,K1615Q,R1616Q )-NLS (TSC2 Human)
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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