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We narrowed to 18,824 results for: nls

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Showing: 1001 - 1020 of 18824 results

  3. pCMV-PE7-P2A-GFP

    Plasmid
    #222996
    Purpose
    Mammalian expression of SpCas9 PE7 prime editor with P2A-EGFP marker
    Depositor
    Brittany Adamson
    Publication
    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE7-P2A-EGFP
    Tags
    P2A-EGFP, SV40 bpNLS, and c-Myc NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    222996-plasmid-map-sequence-id-442553

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  4. DD-Cas9 with filler sequence and Venus (EDCPV)

    Plasmid
    #90085
    Purpose
    This plasmid contains destabilized Cas9 and has Venus after P2A sequence. This vector also contains filler sequence which required to be removed for cloning of desired sgRNA
    Depositor
    Raffaella Sordella
    Publication
    Senturk et al Nat Commun. 2017 Feb 22;8:14370. doi: 10
    Insert
    Cas9
    Use
    CRISPR and Lentiviral
    Tags
    Destabilized Domain and Flag
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    July 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    90085-plasmid-map-sequence-id-182691

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  12. pAAV.cTNT.iCre

    Plasmid
    #69916
    Purpose
    Used to package AAV9:cTNT.iCre, which specifically transduce cardiomyocytes and express Cre recombinase. Only works in vivo.
    Depositor
    William Pu
    Publication
    Lin et al Circ Res. 2015 Jan 2;116(1):35-45. doi:
    Has service
    AAV9
    Insert
    improved cre (iCre) recombinase and tdTomato
    Use
    AAV
    Tags
    The iCre coding frame and tdTomato coding frame i…
    Promoter
    Chicken cardiac troponin T
    Available since
    Dec. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    69916-plasmid-map-sequence-id-472137

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  15. PB_tre_Cas9

    Plasmid
    #126029
    Purpose
    PiggyBac cargo vector expressing doxycycline inducible Cas9
    Depositor
    Mauro Calabrese
    Publication
    Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    humanized S. pyrogenes Cas9
    Hygromycin resistance
    Use
    CRISPR; Piggybac
    Expression
    Mammalian
    Promoter
    EF1 alpha and TRE
    Available since
    June 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    126029-plasmid-map-sequence-id-246111

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  17. pTE4398

    Plasmid
    #74042
    Purpose
    Expresses human codon-optimized LbCpf1 and Lb crRNA.
    Depositor
    Ervin Welker
    Publication
    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Lb crRNA
    LbCpf1
    Use
    CRISPR
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Promoter
    CMV and human U6
    Available since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    74042-plasmid-map-sequence-id-137813

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  18. pdCas9-DNMT3A-EGFP (ANV)

    Plasmid
    #71685
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-EGFP; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Publication
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Human, Synthetic, S. pyogenes)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    71685-plasmid-map-sequence-id-322543

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  19. pSynSRE-T-Luc

    Plasmid
    #60444
    Purpose
    Uses the hamster HMG-CoA synthase promoter sequence (-324/-225) and minimal HMG-CoA sythnase TATA box (-28/+39) to study SREBP-dependent transcriptional activation.
    Depositor
    Timothy Osborne
    Publication
    Dooley et al J Biol Chem. 1998 Jan 16;273(3):1349-56.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HMG-CoA synthase promoter (-324/-225)
    Use
    Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Promoter
    HMG-CoA synthase (-324/-225) & TATAA (-28/+39)
    Available since
    Feb. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60444-plasmid-map-sequence-id-266122

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Showing: 1001 - 1020 of 18824 results