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Showing: 1001 - 1020 of 3104 results
  1. pcDNA3.1-nAChr-Alpha6-GFP

    Plasmid
    #50486
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Use
    Tags
    GFP fusion in M3-M4 loop (after residue A405)
    Expression
    Mammalian
    Mutation
    Gly-Ala-Gly flexible linker flanking the GFP open…
    Promoter
    CMV
    Available since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only
  2. pcDNA3.1-V5H6-mPCSK3 D153A

    Plasmid
    #122681
    Purpose
    Expresses mutated mouse PCSK3 in mammalian cells (aspartic acid at position 153 mutated to alanine via site directed mutagenesis)
    Depositor
    Insert
    Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    changed Aspartic Acid 153 to Alanine
    Promoter
    Available since
    May 16, 2019
    Availability
    Academic Institutions and Nonprofits only
  3. pcDNA3.1-nAChr-Alpha6-YFP

    Plasmid
    #50483
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Use
    Tags
    YFP fusion in M3-M4 loop (after residue A405)
    Expression
    Mammalian
    Mutation
    Gly-Ala-Gly flexible linker flanking the YFP open…
    Promoter
    CMV
    Available since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only
  4. pcDNA3.1-nAChr-Alpha6-CFP

    Plasmid
    #50482
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Use
    Tags
    CFP fusion in M3-M4 loop (after residue A405)
    Expression
    Mammalian
    Mutation
    Gly-Ala-Gly flexible linker flanking the CFP open…
    Promoter
    CMV
    Available since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only
  5. pcDNA3-C17orf53_1-87-mCer

    Plasmid
    #211441
    Purpose
    mammalian expression of the N-terminal amino acids 1-87 of human C17orf53 C-terminally fused to mCerulean
    Depositor
    Insert
    C17orf53 (HROB Human)
    Use
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    Deletion of aa 88-646
    Promoter
    Available since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. pcDNA3-C17orf53_1-87-mVen

    Plasmid
    #211442
    Purpose
    mammalian expression of the N-terminal amino acids 1-87 of human C17orf53 C-terminally fused to mVenus
    Depositor
    Insert
    C17orf53 (HROB Human)
    Use
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Deletion of aa 88-646
    Promoter
    Available since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
  7. pcDNA3.1_mutNSDHL

    Plasmid
    #136428
    Purpose
    Expresses NSDHL harboring all currently known mutations within exons 4 and 6 associated with CHILD syndrome.
    Depositor
    Insert
    human NSDHL with mutations in exon 4 and exon 6 (NSDHL Synthetic, Human)
    Use
    Tags
    V5-His tag
    Expression
    Mammalian
    Mutation
    C>T = A105V; G>C = A182P; G>A = R199H; G…
    Promoter
    CMV
    Available since
    March 5, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 1001 - 1020 of 3104 results