We narrowed to 60,621 results for: tra
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Plasmid#223023PurposeExpress OS9 gene in mesenchymal lineage of Zebrafish.DepositorAvailable SinceFeb. 24, 2025AvailabilityAcademic Institutions and Nonprofits only
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rag2:TSPAN31_CE_pISceI
Plasmid#223025PurposeExpress TSPAN31 gene in mesenchymal lineage of Zebrafish.DepositorAvailable SinceFeb. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-HA-CBS(opt)-FLAG
Plasmid#230088Purposeexpressing human CBSDepositorInsertCBS (CBS Human)
UseLentiviralTagsFLAG and HAExpressionMammalianMutationMultiple wobble mutationsPromoterCMVAvailable SinceJan. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
TFORF0089
Plasmid#142532PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF1000
Plasmid#142251PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPBbleo-TGFa-ScNeo
Plasmid#209904PurposeTo monitor the status of TGFα, the plasmid encodes a recombinant TGFα fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent proteinDepositorInsertTGFa-ScNeo (TGFA Human)
TagsGGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…ExpressionMammalianAvailable SinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr2-TGFa-ScNeo
Plasmid#209908PurposeTo monitor the status of TGFα, the plasmid encodes a recombinant TGFα, fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent proteinDepositorInsertTGFa-ScNeo (TGFA Human)
TagsGGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…ExpressionMammalianAvailable SinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
DG SaCas9 GFP V3
Plasmid#226961PurposeCBh-SaCas9-2A-GFP, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
DG SaCas9 puro V3
Plasmid#226960PurposeCBh-SaCas9-2A-Puro, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
PDG458 eSp(1.1) V3
Plasmid#226964PurposeCBh-eSpCas9(1.1)-2A-GFP, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pICH86966::AtU6p::sgRNA:NbCysP6
Plasmid#223217PurposeExpresses an sgRNA targeting the NbCysP6 gene in Nicotiana benthamiana with an Arabidopsis U6 promoterDepositorInsertNbCysP6 sgRNA
UseCRISPR and Synthetic BiologyTagsNoneExpressionPlantPromoterArabidopsis U6 promoterAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-NEDD4L_ΔC2 (157)
Plasmid#187720PurposeExpress GST-tagged NEDD4L ΔC2 (157) in insect cellsDepositorInsertNEDD4L residues 157-975 (NEDD4L Human)
TagsGST-HRV 3C siteExpressionInsectMutationdeletion of residues 1-156Available SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-NEDD4L_W2-W3-W4-HECT (349)
Plasmid#187721PurposeExpress GST-tagged NEDD4L W2-W3-W4-HECT (349) in insect cellsDepositorInsertNEDD4L residues 349-975 (NEDD4L Human)
TagsGST-HRV 3C siteExpressionInsectMutationdeletion of residues 1-348Available SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBacHTA-NEDD4L_C2-W1-L-W2-HECT
Plasmid#187723PurposeExpress His-tagged NEDD4L C2-W1-L-W2-HECT chimera in insect cellsDepositorInsertNEDD4L residues 1-418+582-975 (NEDD4L Human)
TagsHis-TEVExpressionInsectMutationdeletion of residues 419-581Available SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-NEDD4L_W2-W3-W4-HECT (381)
Plasmid#187708PurposeExpress his-tagged NEDD4L W2-W3-W4-HECT in bacterial cellsDepositorInsertNEDD4L residues 381-975 (NEDD4L Human)
TagsHis-HRV 3C siteExpressionBacterialMutationdeletion of residues 1-380Available SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-NEDD4L_W3-W4-HECT (492)
Plasmid#187709PurposeExpress his-tagged NEDD4L W3-W4-HECT in bacterial cellsDepositorInsertNEDD4L residues 492-975 (NEDD4L Human)
TagsHis-HRV 3C siteExpressionBacterialMutationdeletion of residues 1-491Available SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-NEDD4L_W4-HECT
Plasmid#187710PurposeExpress his-tagged NEDD4L W4-HECT in bacterial cellsDepositorInsertNEDD4L residues 543-975 (NEDD4L Human)
TagsHis-HRV 3C siteExpressionBacterialMutationdeletion of residues 1-542Available SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-NEDD4L_FL-CS
Plasmid#187713PurposeExpress the catalytically inactive GST-tagged NEDD4L FL in bacterial cellsDepositorInsertNEDD4L FL with C874 mutated to a serine (NEDD4L Human)
TagsGST-HRV 3C siteExpressionBacterialMutationCys874 mutated to a serineAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-NEDD4L_W2-W3-W4-HECT (348)
Plasmid#187716PurposeExpress GST-tagged NEDD4L W2-W3-W4-HECT (348) in bacterial cellsDepositorInsertNEDD4L with residues XX (NEDD4L Human)
TagsGST-HRV 3C siteExpressionBacterialMutationdeletion of residues 1-347Available SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-NEDD4L_WW34
Plasmid#187718PurposeExpress GST-tagged NEDD4L WW34 tandem domains in bacterial cellsDepositorInsertNEDD4L residues 492-581 (NEDD4L Human)
TagsGST-HRV 3C siteExpressionBacterialMutationonly residues 492-581Available SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only