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pAAV-CAG-flex-iGluSnFR4s-PDGFR-WPRE

Plasmid

#234447

Purpose

AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

Depositor

GENIE Project

Article

Aggarwal et al Nat Methods. 2026 Feb;23(2):417-425. doi

Insert

iGluSnFR4s-PDGFR

Use

AAV and Cre/Lox

Tags

IgK chain and Myc epi tag-PDGFR TM Domain

Expression

Mammalian

Promoter

CAG

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-dInPAkt

Plasmid

#181834

Purpose

Genetically encoded single-color sensor for monitoring plasma membrane PI(3,4,5)P2 dynamics in living cells. Based on dimerization-dependent red fluorescent protein (ddRFP).

Depositor

Jin Zhang

Article

Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

Insert

Lyn-dInPAkt

Tags

N-terminal targeting sequence from Lyn kinase, dd…

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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EGFR L858R

Plasmid

#11012

Purpose

Retroviral construct for expressing human EGFR with L858R mutation in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR L858R (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

L858R (tumor-derived)

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pMT-Hrp48-ADARcd-V5

Plasmid

#81172

Purpose

identification of cell-specific targets of RNA binding proteins

Depositor

Michael Rosbash

Article

McMahon et al Cell. 2016 Mar 30. pii: S0092-8674(16)30

Insert

Hrp48-ADARcd fusion (Hrb27C Fly)

Expression

Insect

Promoter

Drosophila metallothionein promoter

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Rac1-wt

Plasmid

#12985

Depositor

Gary Bokoch

Insert

Rac1 (RAC1 Human)

Tags

Myc

Expression

Mammalian

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-SFFV-Zim3-dCas9-P2A-BFP

Plasmid

#188767

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9

Use

Lentiviral

Tags

HA-2xNLS and Zim3 KRAB-NLS fusion

Promoter

SFFV

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERH-STRA8

Plasmid

#222585

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of STRA8, with hygromycin resistance

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

STRA8 (STRA8 Human)

Expression

Mammalian

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-AM

Plasmid

#206513

Purpose

AAV packaging vector containing AAV2 rep and AM cap and partial AAV2 p5 promoter

Depositor

Mark Kay

Article

Gonzalez-Sandoval et al Nat Commun. 2023 Apr 28;14(1):2448. doi:

Insert

AM capsid

Use

AAV

Promoter

p5

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pTriEx4-H6-FGAm (FlincG3)

Plasmid

#49202

Purpose

Fluorescent reporter for cGMP

Depositor

John Garthwaite

Article

Bhargava et al Front Mol Neurosci. 2013 Sep 24;6:26. do

Insert

FlincG3

Tags

His tag + other tags

Expression

Bacterial, Insect, and Mamm…

Mutation

Met to Lys in cpEGFP region similar to CGaMP3

Promoter

CMV

Available Since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti_dCas9-KRAB-MeCP2_Blast

Plasmid

#221501

Purpose

Lentiviral construct for expression of the dCas9-KRAB-MeCP2 CRISPRi vector

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

dCas9-KRAB-MeCP2-T2A-Blast

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Promoter

CMV

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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UMsfG

Plasmid

#161005

Purpose

ubiquitous expression of MCP-sfGFP

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

MCP-sfGFP

Expression

Plant

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pGEMS-II-dCas13-APO1-YTH

Plasmid

#204767

Purpose

GEMS system using dCas13-tethered APO1-YTH (pCMV-EGFP-PEST-m6A sensor-DHFR; EF1a-dCas13-NLS-APO1-YTH; hPGK-DsRed)

Depositor

Kate Meyer

Article

Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

dCas13-NLS-APO1-YTH-HA

Use

Synthetic Biology

Expression

Mammalian

Promoter

Ef1alpha

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-Cre-GFP

Plasmid

#68544

Purpose

AAV expression of Cre-GFP fusion

Depositor

Eric Nestler

Article

Kennedy et al Nat Neurosci. 2013 Apr;16(4):434-40. doi

Insert

Cre

Use

AAV and Cre/Lox

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pSC189

Plasmid

#32114

Depositor

Eric Rubin

Article

Chiang et al Gene. 2002 Aug 21;296(1-2):179-85.

Type

Empty backbone

Use

Transposon delivery vector

Tags

3x FLAG

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pLV GG hUbC-dsRED

Plasmid

#84034

Purpose

Contains Golden Gate cassette to express up to four gRNAs and dsRED

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pIDTsmart-RC2-delVP1-VP1-588TAG

Plasmid

#240122

Purpose

Express AAV2 capsid with ncAA mutant at site 588 of VP1

Depositor

Abhishek Chatterjee

Article

Pham et al Mol Ther Oncol. 2025 Aug 23;33(4):201040

Inserts

RC2-DelVP1
VP1-588-TAG

Use

AAV

Expression

Mammalian

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-UTX

Plasmid

#24168

Purpose

Mammalian expression of human UTX with HA tag

Depositor

Kristian Helin

Article

Agger et al Nature. 2007 Oct 11. 449(7163):731-4.

Insert

UTX (KDM6A Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pcsII-pb4-flag

Plasmid

#86768

Purpose

C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

Depositor

Do-Hyung Kim

Article

Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

Insert

proteasome beta subunit 4 (PSMB4 Human)

Use

Lentiviral

Tags

flag

Expression

Mammalian

Promoter

EF-1a

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pH6-MBP-TEV-BirA

Plasmid

#179694

Purpose

Expresses His6 tagged MBP-BirA fusion protein

Depositor

Mark Arbing

Insert

BirA

Tags

His6-MBP

Expression

Bacterial

Available Since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC2A5

Plasmid

#132090

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC2A5 (SLC2A5 Human)

Expression

Mammalian

Available Since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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