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pFUW-tetO-Irf2

Plasmid

#139814

Purpose

doxycycline-inducible expression of mouse Irf2 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Irf2 (Irf2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mClover3-MAP4-MTBD

Plasmid

#171498

Purpose

T7 promotor drives in vitro transcription of mClover3-tagged mouse MAP4-Microtubule Binding Domain mRNA

Depositor

Melina Schuh

Article

So et al Science. 2019 Jun 28;364(6447). pii: 364

Insert

MAP4-MTBD (Map4 Mouse)

Use

Pgehme

Tags

mClover3

Promoter

T7

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-AID-PcrA-UGI-T2A-mTagBFP2

Plasmid

#221224

Purpose

Express HACE Editor as a fusion of AID cytidine deaminase and PcrA helicase with mTagBFP2 reporter

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

AID-PcrA-UGI-T2A-mTagBFP2

Expression

Mammalian

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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VZV ORF 37(gH)

Plasmid

#64081

Purpose

eukaryotic expression plasmid for VZV glycoprotein H

Depositor

Randall Cohrs

Article

Birlea et al J Virol. 2013 Jan;87(1):415-21. doi: 10.

Insert

VZV glycoprotein H

Expression

Mammalian

Promoter

CMV IE with intron

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only
pET48 AtGUN1-PS

Plasmid

#136358

Purpose

Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 918)

Depositor

Tatsuru Masuda

Article

Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

Insert

cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 918 (GUN1 Mustard Weed)

Tags

TRX-His

Expression

Bacterial

Promoter

T7

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pBetaActin-FKBP-mScarlet-KIFC1motor

Plasmid

#191334

Purpose

Expresses the fluorescently labeled minus-end directed KIFC1 motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FKBP-mScarlet-KIFC1 (KIFC1 Human)

Expression

Mammalian

Mutation

motor domain aa 125-673; N593K

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv1_SDHA

Plasmid

#177980

Purpose

lentiviral vector expressing Cas9 and a sgRNA targeting SDHA

Depositor

David Sabatini, Jessica Spinelli

Article

Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

Insert

sgRNA targeting SDHA (SDHA Human)

Use

Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

U6

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pHS1928

Plasmid

#205967

Purpose

JAGBLH010000220 Cas8-HNH operon (E. coli codon optimized) with His-tagged Cas8 in pET45b(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas8-HNH all proteins, with His-tagged Cas8

Tags

14xHis on Cas8

Expression

Bacterial

Promoter

T7

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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CD45 sp-SNAPf-CD45R0Ex-His10 (XSB316)

Plasmid

#78509

Purpose

Construct for expressing CD45R0 (extracellular domain) His10 in Sf9

Depositor

Ron Vale

Article

Su et al Science. 2016 Apr 29;352(6285):595-9. do

Insert

CD45R0Ex (PTPRC Human)

Tags

His10 and SNAPf

Expression

Insect

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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IMPT-2070

Plasmid

#172092

Purpose

pFastBac1 vector with GP64 promoter replacing polH promoter expressing A2aAR+BRIL GPCR

Depositor

Raymond Stevens

Article

Liu et al Science. 2012 Jul 13;337(6091):232-6. do

Insert

Homo sapiens adenosine A2a receptor (ADORA2A) (ADORA2A Human)

Tags

cytochrome b562RIL

Expression

Insect

Promoter

gp64

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pHS1950

Plasmid

#205983

Purpose

Ga0116245_10002768 T7/LacO-Cas6 + TwinStrep-Cas14 + Cas7 + His14-Cas5::pJ23119-DR-Fn spacer-DR

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Inserts

Ga0116245_10002768 candidate type VII proteins
Cas14
Cas5
CRISPR array

Tags

14xHis and TwinStrep-tag

Expression

Bacterial

Promoter

T7 (proteins), pJ23119 (crRNA)

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-TFEC

Plasmid

#178447

Purpose

doxycycline-inducible expression of Human TFEC in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

TFEC (TFEC Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTol2-elavl3-Voltron

Plasmid

#119037

Purpose

Pan neuronal expression of Voltron in zebrafish

Depositor

Eric Schreiter

Article

Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

Insert

Voltron

Use

Zebrafish expression

Promoter

elavl3

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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RV-Cag-Dio-EYFP-P2A-cMaf

Plasmid

#98174

Purpose

Retroviral vector encoding Cre-dependent expression of EYFP-P2A-Maf

Depositor

Oscar Marin

Article

Mi et al Science. 2018 Feb 22. pii: science.aar68

Insert

cMaf (codon optimzed) (Maf Mouse)

Use

Retroviral

Mutation

Codon optimized

Promoter

CAG

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG -YWAA-pGK-HYG

Plasmid

#107381

Purpose

Mammalian expression of FLAG-YWAA

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

cereblon (CRBN Human)

Tags

FLAG

Expression

Mammalian

Mutation

CRBN-Y383A/W385A

Promoter

CMV

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-Tet2 promoter F

Plasmid

#63879

Purpose

Luciferase reporter plasmid used to study transcriptional control of murine Tet2

Depositor

Kian Peng Koh

Article

Sohni et al Mol Cell Biol. 2015 Mar 15;35(6):1026-42

Insert

Tet2 Promoter fragment F

Use

Luciferase

Expression

Mammalian

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-GST FNIP2

Plasmid

#164982

Purpose

FNIP2 expressing vector for mammalian cells

Depositor

Roberto Zoncu

Article

Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

Insert

FNIP2 (FNIP2 Human)

Tags

GST

Expression

Mammalian

Promoter

CAG

Available Since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-Fgß-ddFLN4-HIS-ybbr

Plasmid

#101239

Purpose

wildtype Fgß peptide with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces

Depositor

Hermann Gaub

Article

Milles et al Science. 2018 Mar 30;359(6383):1527-1533

Insert

Fgß peptide, ddFLN4 fingerprint (FGB Human, D. discoideum)

Tags

HIS and ybbr

Expression

Bacterial

Mutation

C18S in ddFLN4

Promoter

T7

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pHS0781 pcDNA3.1(+) CMV-SV40 NLS-AwaIscB-nucleoplasmin NLS-3xHA

Plasmid

#176538

Purpose

Mammalian expression of nuclear localized AwaIscB

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

AwaIscB

Tags

Nucleoplasmin NLS-3xHA and SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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p667-UBC-NRF1-V5-miniTurbo

Plasmid

#207810

Purpose

C-terminal tagged NRF1 for miniTurbo proximity experiments

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

NRF1-V5-miniTurbo (NFE2L1 Human)

Use

Lentiviral

Tags

V5-miniTurbo

Promoter

UBC

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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