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pHAGE TRE dCas9-VP64

Plasmid

#50916

Purpose

Tet-regulatable dCas9-VP64 2nd generation lentiviral expression vector

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xHA and VP64

Expression

Mammalian

Mutation

D10A, H840A nuclease-deficient

Promoter

TRE

Available Since

March 18, 2014

Availability

Academic Institutions and Nonprofits only

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Flag-FOXK2

Plasmid

#153133

Purpose

Expression of Flag tagged human FOXK2 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXK2 (FOXK2 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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Flag-GFP11-pDEST-N

Plasmid

#118366

Purpose

Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible N-terminal vector with BiFC-tag (GFP11-Flag) to screen interaction partners.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

GFP11-Flag

Expression

Mammalian

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pDY1440 STITCHR GFP NOLC1 payload

Plasmid

#234829

Purpose

GFP cargo with 50bp NOLC homology arms

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

Insert

copGFP

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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VEE-mScarlet3-IRES-EGFP-IRES-moxBFP

Plasmid

#242407

Purpose

Native (conventional) self-amplifying RNA (saRNA) control construct expresses moxBFP as visual marker of IRES functionality

Depositor

Ewan St. John Smith

Article

Lim et al bioRxiv 2024.09.24.614636

Inserts

mScarlet3
IRES-EGFP
IRES-moxBFP

Use

Self-amplifying rna

Expression

Mammalian

Available Since

Sept. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TYMS

Plasmid

#100545

Purpose

mammalian expression of thymidylate synthetase

Depositor

Yuh-Shan Jou

Article

Yeh et al Oncotarget. 2017 Sep 8;8(44):77734-77751

Insert

thymidylate synthetase (TYMS Human)

Expression

Mammalian

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pZR047_Lenti-U6-sgGARP-MS2-PP7-hPGK-PCP-p65-HSF1-Puro

Plasmid

#180268

Purpose

Lentiviral expression vector for CRISPRa-SAM with GARP targeting sgRNA

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

GARP sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Split random

Plasmid

#200646

Purpose

Expressed a eGFP sequence where a chimeric intron with a random sequence was inserted

Depositor

Claire Francastel

Article

Bogard et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

Insert

Split GFP with chimeric intron containing random sequence

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pEF Synaptophysin-mRuby

Plasmid

#188980

Purpose

Synaptic buton marker

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Synaptophysin (Syp Synthetic, Rat)

Expression

Mammalian

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_NDG

Plasmid

#26820

Depositor

Derrick Rossi

Article

Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

Insert

5'UTR-NDGFP-3'UTR

Expression

Mammalian

Mutation

nuclear destabilized eGFP

Available Since

Nov. 17, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pLOX-TERT-iresTK

Plasmid

#12245

Purpose

2nd generation lentiviral vector for reversible immortalization of human primary cells

Depositor

Didier Trono

Article

Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

Insert

TERT, HSV1-TK (TERT Human)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Mutation

LoxP in 3' LTR.

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pCAGGS-N-TEV-Twin-Strep

Plasmid

#202526

Purpose

Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in mammalian cell

Depositor

Chiaki Murakami

Article

Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

Type

Empty backbone

Tags

TEV cleavable site and Twin-strep-tag

Expression

Mammalian

Promoter

CAG

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pSLIK-Hygro

Plasmid

#25737

Purpose

3rd generation Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Hygromycin resistance gene coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3/Myc-DNMT3B1

Plasmid

#35522

Purpose

Mammalian expression of human DNMT3B1 with myc tag

Depositor

Arthur Riggs

Article

Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

Insert

DNMT3B1 (DNMT3B Human)

Tags

Myc

Expression

Mammalian

Available Since

March 9, 2012

Availability

Academic Institutions and Nonprofits only

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pDGE347

Plasmid

#153228

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV2.5-THP-GFP

Plasmid

#80336

Purpose

Fluorescent Reporter for Dopaminergic Neuron Differentiation

Depositor

Kwang-Soo Kim

Article

Oh et al Gene Ther. 2009 Mar;16(3):437-40. doi: 1

Insert

rat TH promoter (Th Rat)

Use

AAV

Promoter

rat Tyrosine hydroxylase

Available Since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE334

Plasmid

#153243

Purpose

M2E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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10xHis-MBP-TEV-S. pyogenes dCas9 M1C D10A C80S H840A C574S

Plasmid

#60815

Purpose

dCas9 with single cysteine residue (M1C) for site-specific labelling

Depositor

Jennifer Doudna

Article

O'Connell et al Nature. 2014 Sep 28. doi: 10.1038/nature

Insert

dCas9 M1C D10A C80S H840A C574S

Tags

His10 and MBP

Expression

Bacterial

Mutation

M1C D10A C80S H840A C574S

Available Since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only

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RIP Cre-ER (DM#265)

Plasmid

#15029

Depositor

Douglas Melton

Article

Dor et al Nature. 2004 May 6. 429(6987):41-6.

Insert

Rat insulin promoter (RIP) (Ins2 Rat)

Use

Cre/Lox; For making transgenic constructs

Tags

Cre-ER (TM)

Expression

Mammalian

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only

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pSLIK-Zeo

Plasmid

#25736

Purpose

Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Zeocin resistance gene coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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