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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 5,632 results for: chia

Showing: 2551 - 2600 of 5632 results

  1. pJK591

    Plasmid
    #71589
    Purpose
    Produces Acetobacter aceti 1023 acetyl-CoA:oxalate CoA-transferase (UctC) with Thr2>Ala/Asp177>Ala mutants (UctC-T2A,D177A)
    Depositor
    T. J. Kappock
    Article
    Mullins et al PLoS One. 2013 Jul 23;8(7):e67901. doi:
    Insert
    acetyl-CoA:oxalate CoA-transferase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes threonine-2 to alanine and aspartate-177 …
    Promoter
    T7
    Available since
    Jan. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    71589-plasmid-map-sequence-id-130927

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  2. pHL 100

    Plasmid
    #52989
    Purpose
    pLlac0-1:OxyS-mCherry, pLtetO-1:fhlA::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    OxyS sRNA
    fhlA
    Use
    Synthetic Biology
    Tags
    GFP and RBS-mCherry
    Expression
    Bacterial
    Mutation
    −309 to +96 bp relative to start codon
    Promoter
    pLlac0-1 and pLtetO-1
    Available since
    Jan. 27, 2015
    Availability
    Academic Institutions and Nonprofits only

  3. pHL 802

    Plasmid
    #52991
    Purpose
    pLtetO-1:OxyS-mCherry, pLlacO-1:ompC::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    OxyS sRNA
    ompC
    Use
    Synthetic Biology
    Tags
    GFP and RBS-mCherry
    Expression
    Bacterial
    Mutation
    contains −81 to +36 bp relative to start codon
    Promoter
    pLlacO-1 and pLtetO-1
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  4. pHL 1070

    Plasmid
    #53002
    Purpose
    pCon:DsrA, pLlacO-1:sodB::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    DsrA sRNA
    sodB
    Use
    Synthetic Biology
    Tags
    GFP
    Expression
    Bacterial
    Mutation
    contains −56 to +141 bp relative to start codon
    Promoter
    pCon and pLlacO-1
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  12. pEcCas

    Plasmid
    #73227
    Purpose
    Constitutive expression of cas9 and inducible expression of lambda RED and sgRNA
    Depositor
    Sheng Yang
    Article
    Li et al Acta Biochim Biophys Sin (Shanghai). 202
    Inserts
    Cas9
    sgRNA
    Use
    CRISPR; Red recombinase
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    native promoter
    Available since
    June 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    73227-plasmid-map-sequence-id-419024

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  15. pMS_366

    Plasmid
    #182131
    Purpose
    expresses retron RNA, ec.86 Reverse Transcriptase, and CspRecT SSAP, to create a specific rifampicin resistance mutation
    Depositor
    George Church
    Article
    Schubert et al Proc Natl Acad Sci U S A. 2021 May 4;118
    Inserts
    retron RNA
    ec.86 RT
    CspRecT
    Use
    Tags
    Expression
    Mutation
    incorporated rifampicin-resistance-conferring don…
    Promoter
    Available since
    Sept. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    182131-plasmid-map-sequence-id-355782

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  18. pREDCas9

    Plasmid
    #71541
    Purpose
    Constitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.
    Depositor
    Tao Chen
    Article
    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10
    Inserts
    Cas9
    lambda red genes
    plasmid curing system
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    July 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71541-plasmid-map-sequence-id-333602

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  20. Plasmid EUPp_09

    Plasmid
    #157745
    Purpose
    PgyrA_ada _adh2 _pMB1_ampR (for expressing two enzymes (Ada and Adh2) to allow E. coli to consume ethanol)
    Depositor
    Kang Zhou
    Article
    Liang et al Metab Eng. 2020 Nov 25. pii: S1096-7176(
    Inserts
    acetaldehyde dehydrogenase
    alcohol dehydrogenase (ADH2 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PgyrA
    Available since
    June 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    157745-plasmid-map-sequence-id-334102

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  28. pCas9cur

    Plasmid
    #71538
    Purpose
    Constitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.
    Depositor
    Tao Chen
    Article
    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10
    Inserts
    Cas9
    Plasmid curing system
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    71538-plasmid-map-sequence-id-129198

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  29. pTA-Mob 2.0

    Plasmid
    #149662
    Purpose
    Conjugation helper plasmid for use in cis (self-transfer) and trans (transfer of plasmids with RK2/RP4 origin of transfer) mobilization to prokaryotic and eukaryotic cells.
    Depositor
    Bogumil Karas
    Article
    Soltysiak et al Int J Mol Sci. 2019 Oct 21;20(20). pii:
    Type
    Empty backbone
    Use
    Synthetic Biology; Conjugation helper plasmid
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    149662-plasmid-map-sequence-id-379005

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  44. p46Cpf1-OP2

    Plasmid
    #98592
    Purpose
    Produces lambda Red and Cpf1 for recombination and selection. The plasmid is combined with a donor plasmid (with crRNA and template) for rapid genome editing in E.coli.
    Depositor
    Qiong Wu
    Article
    Ao et al Front Microbiol. 2018 Oct 9;9:2307. doi:
    Insert
    FnCpf1
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Codon optimized for E. coli
    Promoter
    araBAD
    Available since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    98592-plasmid-map-sequence-id-201329

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  47. RBD#4L

    Plasmid
    #193021
    Purpose
    hnRNP C RRM RNA binding domain inserted in between N415 and N416 of Loop 1 of LwaCas13a
    Depositor
    Xue Gao
    Article
    Yang et al Nat Chem Biol. 2022 Sep 22. pii: 10.1038
    Insert
    Cas13a
    Use
    Tags
    hnRNP C RRM
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    193021-plasmid-map-sequence-id-380240

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  50. pTJV1Sc-rpoB

    Plasmid
    #166978
    Purpose
    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance
    Depositor
    Christopher Reisch
    Article
    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00
    Inserts
    Ec86 Retron (ECD_00831 )
    lambda bet
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23101 and VanCC
    Available since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    166978-plasmid-map-sequence-id-331328

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Showing: 2551 - 2600 of 5632 results