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pCMVd1-NLS-GAL4-iLight2-VP16-T2A-mTagBFP2

Plasmid

#219548

Purpose

NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2 construct expression in mammalian cells.

Depositor

Vladislav Verkhusha

Article

Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

Insert

NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2

Expression

Mammalian

Mutation

The (SAGG)x4 linker between GAL4(1-65) and the PC…

Promoter

CMVd1

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCSII-EF-miRFP709-hCdt(1/100)

Plasmid

#80007

Purpose

hCdt(1/100) fused with near-infrared fluorescent protein miRFP709 (G1 phase cell cycle reporter)

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

hCdt1(1/100) (CDT1 Human)

Use

Lentiviral

Tags

miRFP709

Promoter

EF-1 alpha

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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phTERT-cp PS Intein tTA

Plasmid

#242032

Purpose

Blue-light-controlled, hTERT-promoter-driven release of the tTA transactivator from cytoplasm to nucleus.

Depositor

Vladislav Verkhusha

Article

Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

Insert

Cp PS Intein tTA

Expression

Mammalian

Mutation

The CMV promoter was replaced with the hTERT prom…

Promoter

hTERT

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-GAL4-iLight-VP16

Plasmid

#170274

Purpose

NLS-Gal4(1-65)-iLight(human codon optimized)-VP16 construct expression in mammalian cells. pAAV vector.

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

Insert

NLS-Gal4(1-65)-iLight(human codon optimized)-VP16

Use

AAV

Mutation

See Comments

Promoter

CaMKII

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-CIB1-Myc

Plasmid

#32505

Depositor

John Groffen, Nora Heisterkamp

Article

Haataja et al J Biol Chem. 2002 Mar 8;277(10):8321-8.

Insert

CIB1 (CIB1 Human)

Tags

Myc

Expression

Mammalian

Available Since

Jan. 26, 2012

Availability

Academic Institutions and Nonprofits only
pAAV-CaMKII-iGECI-NES

Plasmid

#160424

Purpose

AAV plasmid for CaMKII-driven expression of a genetically-encoded calcium indicator iGECI with a nuclear export signal sequence

Depositor

Vladislav Verkhusha

Article

Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

Insert

iGECI-NES

Use

AAV

Expression

Mammalian

Promoter

CaMKII

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pEB3-miRFP670nano

Plasmid

#127433

Purpose

EB3 (microtubule-associated protein) labeling with near-infrared fluorescent protein miRFP670nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

EB3-miRFP670nano (MAPRE3 Synthetic, Human)

Tags

miRFP670nano

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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eDrEGFR

Plasmid

#184000

Purpose

Optically controlled eDrEGFR expression in mammalian cells

Depositor

Dmitry Gerashchenko, Vladislav Verkhusha

Article

Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

Insert

eDrEGFR (EGFR Human)

Tags

Ig kappa secretory signal, mCherry, Deinococcus r…

Expression

Mammalian

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pEB3-miRFP703

Plasmid

#79994

Purpose

EB3 (microtubule-associated protein) labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

EB3 (MAPRE3 Human)

Tags

miRFP703

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pMito-LSSmKate2-N1

Plasmid

#31879

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

human cytochrome C oxidase subunit VIII (COX8A Human)

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

Available Since

Sept. 9, 2011

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1Xpress-BcrGAP

Plasmid

#38175

Depositor

Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

BCR (BCR Human)

Tags

Xpress

Expression

Mammalian

Mutation

contains GAP domain amino acids 1004-1271

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
anxA10-GFP

Plasmid

#107199

Purpose

Expresses human annexin A10-GFP fusion protein for live cell imaging

Depositor

Volker Gerke, Ursula Rescher

Article

Quiskamp et al Cell Mol Life Sci. 2014 Jan;71(2):311-29

Insert

annexin A10 (ANXA10 Human)

Tags

Green Fluorescent Protein

Expression

Mammalian

Promoter

CMV

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1Xpress-AbrGAP

Plasmid

#38177

Depositor

Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

Abr GAP domain (ABR Human)

Tags

Xpress

Expression

Mammalian

Mutation

ABR GAP domain including amino acids 597-859

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
eDrcKIT

Plasmid

#184001

Purpose

Optically controlled eDrcKIT expression in mammalian cells

Depositor

Dmitry Gerashchenko, Vladislav Verkhusha

Article

Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

Insert

eDrcKIT (KIT Human)

Tags

Ig kappa secretory signal, mCherry, Deinococcus r…

Expression

Mammalian

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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ECFP-Bcr in pCCL-cppt178-MNDU3

Plasmid

#38156

Depositor

Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

Bcr (BCR Human)

Use

Lentiviral

Tags

ECFP

Available Since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only
pB2AR-miRFP670nano

Plasmid

#127442

Purpose

Beta-2-adrenergic receptor internally tagged with miRFP670nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

B2AR-miRFP670nano (ADRB2 Synthetic, Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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pRD408

Plasmid

#178199

Purpose

Expression of H-NS from its natural promoter

Depositor

Remus Dame

Article

Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

Insert

phns + hns (hns Escherichia coli)

Expression

Bacterial

Promoter

hns promoter

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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ECFP-Bcr NE/AA

Plasmid

#36418

Depositor

John Groffen, Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

breakpoint cluster region (BCR Human)

Tags

ECFP

Expression

Mammalian

Mutation

N689A/ E690A

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
pCDNA3.1Xpress-Abr-HisC

Plasmid

#32509

Depositor

John Groffen, Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

ABR (ABR Human)

Tags

HISC

Expression

Mammalian

Available Since

Feb. 13, 2012

Availability

Academic Institutions and Nonprofits only
Bcr DH domain NE/AA pGEX

Plasmid

#38180

Depositor

Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

BCR DH domain N689A/E690A (BCR Human)

Tags

GST

Expression

Bacterial

Mutation

BCR DH domain N689A/E690A

Available Since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only