We narrowed to 2,498 results for: ERK

1. anxA2-GFP

   Plasmid

   #107196

   Purpose

   Expresses human annexin A2-GFP fusion protein for live cell imaging

   Depositor

   Ursula Rescher, Volker Gerke

   Article

   Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

   Insert

   annexin A2 (ANXA2 Human)
   

   Use

   Tags

   Green Fluorescent Protein

   Expression

   Mammalian

   Mutation

   Ala residue 65 in human sequence replaced by Glu …

   Promoter

   CMV

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. EGFP-Bcr R1090A

   Plasmid

   #36416

   Depositor

   Nora Heisterkamp, Eunjoon Kim

   Article

   Oh et al J Neurosci. 2010 Oct 20;30(42):14134-44.

   Insert

   breakpoint cluster region protein isoform 1 (BCR Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   R1090A

   Promoter

   Available since

   Aug. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-CARD14-E138A

   Plasmid

   #69921

   Purpose

   Expresses mutant FLAG-CARD14 in mammalian cells

   Depositor

   Francesca Capon

   Article

   Berki et al J Invest Dermatol. 2015 Jul 23. doi: 10.

   Insert

   CARD14 isoform 3 (CARD14 Human)
   

   Use

   Tags

   FLAG

   Expression

   Bacterial and Mammalian

   Mutation

   E138A

   Promoter

   CMV

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3-CARD14-G117S

   Plasmid

   #69920

   Purpose

   Expresses mutant FLAG-CARD14 in mammalian cells

   Depositor

   Francesca Capon

   Article

   Berki et al J Invest Dermatol. 2015 Jul 23. doi: 10.

   Insert

   CARD14 isoform 3 (CARD14 Human)
   

   Use

   Tags

   FLAG

   Expression

   Bacterial and Mammalian

   Mutation

   G117S

   Promoter

   CMV

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3-CARD14-D176H

   Plasmid

   #69919

   Purpose

   Expresses mutant FLAG-CARD14 in mammalian cells

   Depositor

   Francesca Capon

   Article

   Berki et al J Invest Dermatol. 2015 Jul 23. doi: 10.

   Insert

   CARD14 isoform 3 (CARD14 Human)
   

   Use

   Tags

   FLAG

   Expression

   Bacterial and Mammalian

   Mutation

   D176H

   Promoter

   CMV

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3-CARD14-L156P

   Plasmid

   #69918

   Purpose

   Expresses mutant FLAG-CARD14 in mammalian cells

   Depositor

   Francesca Capon

   Article

   Berki et al J Invest Dermatol. 2015 Jul 23. doi: 10.

   Insert

   CARD14 isoform 3 (CARD14 Human)
   

   Use

   Tags

   FLAG

   Expression

   Bacterial and Mammalian

   Mutation

   L156P

   Promoter

   CMV

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

7. Bcr/Abl P210-pLEF

   Plasmid

   #38158

   Depositor

   Nora Heisterkamp

   Article

   Kweon et al J Biol Chem. 2008 Feb 8;283(6):3023-30.

   Insert

   BCR/ABL P210 (ABL1, BCR Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Aug. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. Bcr/Abl P190-pLEF

   Plasmid

   #38159

   Depositor

   Nora Heisterkamp

   Article

   Kweon et al J Biol Chem. 2008 Feb 8;283(6):3023-30.

   Insert

   BCR/ABL P190 (ABL1, BCR Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   T117M in c-ABL1

   Promoter

   SV40

   Available since

   Aug. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLEVI(408)-ColE-iLight-msfGFP

   Plasmid

   #170268

   Purpose

   Expresses LexA_DBD-iLight-msfGFP under J23116 promoter and mCherry under ColE promoter with a LexA408 operator in bacteria.

   Depositor

   Vladislav Verkhusha

   Article

   Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

   Insert

   iLight
   

   Use

   Tags

   msfGFP

   Expression

   Bacterial

   Mutation

   To design iLight for expression in bacteria, a fu…

   Promoter

   pJ23116

   Available since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a(+)-SpdCas9-SBiT_Strep-Tag-II

    Plasmid

    #198750

    Purpose

    Expresses S. Pyogenes dCas9 fused to SmallBiT fragment of split NanoLuc luciferase

    Depositor

    Maarten Merkx

    Article

    van der Veer et al ACS Cent Sci. 2023 Mar 15;9(4):657-667.

    Insert

    SpdCas9 - SmallBiT
    

    Use

    Tags

    Strep-tag II

    Expression

    Bacterial

    Mutation

    D10A, H840A (nuclease inactivating mutations in &…

    Promoter

    T7

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a(+)-SpdCas9-LBiT_Strep-Tag-II

    Plasmid

    #198751

    Purpose

    Expresses S. Pyogenes dCas9 fused to LargeBiT fragment of split NanoLuc luciferase

    Depositor

    Maarten Merkx

    Article

    van der Veer et al ACS Cent Sci. 2023 Mar 15;9(4):657-667.

    Insert

    SpdCas9 - LargeBiT
    

    Use

    Tags

    Strep-tag II

    Expression

    Bacterial

    Mutation

    D10A, H840A (nuclease inactivating mutations in &…

    Promoter

    T7

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. cmamxA2-GFP

    Plasmid

    #107197

    Purpose

    Expresses human annexin A2-GFP fusion protein for live cell imaging, all type II Ca2+ binding sites deleted

    Depositor

    Ursula Rescher, Volker Gerke

    Article

    Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

    Insert

    cmannexin A2 (ANXA2 Human)
    

    Use

    Tags

    Green Fluorescent Protein

    Expression

    Mammalian

    Mutation

    all type II Ca2+ binding sites deleted ( D161A, E…

    Promoter

    CMV

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBabe-kappa-HA-dL5-myc-ADRB2

    Plasmid

    #101253

    Purpose

    Expresses HA-dL5(E52D)-cMyc N-terminal fusion of beta-2 adrenergic receptor in mammalian cells, MMLV retroviral plasmid (MBIC5, dL5**, FAP, ADRB2)

    Depositor

    Marcel Bruchez

    Article

    Perkins et al Biochemistry. 2018 Feb 6;57(5):861-871.

    Insert

    kappa-HA-dL5-myc-ADRB2 (ADRB2 Human, Budding Yeast)
    

    Use

    Retroviral

    Tags

    FAP-dL5 and HA epitope

    Expression

    Mammalian

    Mutation

    Promoter

    MMLV LTR

    Available since

    Oct. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pWZL Neo Myr Flag MAPK12

    Plasmid

    #20592

    Depositor

    William Hahn, Jean Zhao

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    MAPK12 (MAPK12 Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pDONR223-MAPK12

    Plasmid

    #23719

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    MAPK12 (MAPK12 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pREPROII-attB

    Plasmid

    #86557

    Purpose

    Dox inducible expression of reprogramming factors (Klf4, Sox2, Oct4, & cMyc), integration via phiC31 attB sites, excision via Cre/loxP

    Depositor

    Ralf Kuehn

    Article

    Merkl et al PLoS One. 2013;8(1):e55170. doi: 10.1371

    Inserts

    Oct4 (Pou5f1 Mouse)
    cMyc (Myc Mouse)
    Sox2 (Sox2 Mouse)
    Kruppel-like factor 4 (Klf4 Mouse)
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FUW-ubiquitin-EphB2-SV40-GFP (B2G)

    Plasmid

    #65442

    Purpose

    Expression of mouse EPHB2 and GFP in mammalian cells

    Depositor

    Eduard Batlle

    Article

    Cortina et al Nat Genet. 2007 Nov;39(11):1376-83. Epub

    Insert

    EPHB2 (Ephb2 Mouse)
    

    Use

    Lentiviral

    Tags

    SV40-EGFP

    Expression

    Mammalian

    Mutation

    See depositor comments below.

    Promoter

    UBQ

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-UBC-S2-RAB_EKARev

    Plasmid

    #160726

    Purpose

    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK indicator), VSV-G tag, and S2 protein scaffold.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    S2-RAB_EKARev
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human ubiquitin C (UBC) promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-UBC-S3-RAB_EKARev

    Plasmid

    #160727

    Purpose

    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK indicator), VSV-G tag, and S3 protein scaffold.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    S3-RAB_EKARev
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human ubiquitin C (UBC) promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Lyn-FLINC-EKAR1

    Plasmid

    #87707

    Purpose

    Fluorescent biosensor for super-resolution imaging of ERK activity; targeted to plasma membrane.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

    Insert

    Lyn-FLINC-EKAR1
    

    Use

    Tags

    N-terminal targeting sequence from Lyn kinase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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