We narrowed to 4,840 results for: donor
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Plasmid#136936Purposedonor plasmid for inducible transgene expression in human cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertall-in-one tet-on system
UseSynthetic BiologyExpressionMammalianPromoterTRE3GSAvailable sinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDY32 AAVS1-PuroR-9xTet0-minCMV-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
Plasmid#161928PurposeDonor plasmid for integrating minCMV promoter-driven dual surface marker/fluorescent reporter at AAVS1 locusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSurface marker and Citrine activation reporter
UseSynthetic BiologyExpressionMammalianPromoterpEFAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PDX1-2A-eGFP
Plasmid#66964PurposePDX1-eGFP mini-vector donor for integration of the eGFP reporter into the PDX1 locusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertpancreatic and duodenal homeobox 1 (PDX1 Human)
UseUnspecifiedTags2A-eGFPAvailable sinceJuly 24, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AL62_pDonor.DMD.TS
Plasmid#100287PurposeDMD intron 43-targeting plasmidDepositorInsertEGFP expression unit (DMD Synthetic, Human)
UseCRISPR and Synthetic Biology; Human targetingExpressionMammalianAvailable sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJT039 AAVS1-PuroR-9xTet0-pEF-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
Plasmid#161927PurposeDonor plasmid for integrating pEF promoter-driven dual surface marker/fluorescent reporter at AAVS1 locusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSurface marker and Citrine repression reporter
UseSynthetic BiologyExpressionMammalianPromoterpEFAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEN366 - pTRE3G-CTCF-mRuby2-BGHpA-CAGGS-rtta3G-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE donor
Plasmid#156432PurposeVector to introduce a constitutive rtta3G cassette and a DOX-inducible wild-type CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCTCF, rtta3G, TetO3G (Ctcf Mouse)
UseMouse TargetingExpressionMammalianMutationnoneAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
qTAG-N-Puro-dTAG
Plasmid#207694PurposeEmpty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-dTAG cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteExpressionMammalianAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAC155-pCR8-sgExpression
Plasmid#49045PurposeU6-driven sgRNA expressing cassette on a gateway donor vectorDepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterU6Available sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMPRAv3:minP-GFP
Plasmid#109036PurposeMPRA v3 minimal promoter with GFP donorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertemGFP
ExpressionMammalianPromoterminPAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAVS1-Puro cTnT FUCCI
Plasmid#136935Purposedonor plasmid for cardiac-specific FUCCI expression in human cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTroponin T promoter
UseSynthetic BiologyExpressionMammalianPromotercTnTAvailable sinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAC149-pCR8-dCas9VP160
Plasmid#48221PurposedCas9VP160 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9(D10A;H840A) fusion with VP160 activation domain
UseCRISPR and Gateway CloningTagsHA Tag and VP160MutationD10A;H840A nuclease-deficientAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONR-53BP1 1-1972 WT
Plasmid#53446PurposeRecombinational donor/master vectorDepositorInsert53BP1 (TP53BP1 Human)
UseGateway CloningMutationsiRNA resistant and mutations A128V, T720A, A1347…Available sinceApril 10, 2015AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pBR_attB(bxb)_lox
Plasmid#183762PurposeBxb1-specific donor plasmid for the cloning of payloads <20 kbDepositorTypeEmpty backboneUseCre/Lox and Synthetic BiologyExpressionMammalianAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
T-2A-EGFP-PGK-Puro
Plasmid#83344PurposeDonor vector to knocin EGFP into human Brachyury(T) locusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHuman Brachyury knockin homology arms (TBXT Human)
Tags2A-EGFPAvailable sinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBFC0996
Plasmid#186695PurposeVcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, 2xLguI cargo stuffer, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right EndDepositorInsertstnsA
tnsB
tnsC
tnsD
tniQ
cas8-cas5 fusion
cas7
cas6
crRNA
Tn7 transposon
2xLguI Cargo Stuffer
SbfI+AsiSI dual restriction site for donor plasmid removal during ET-Seq
ExpressionBacterialPromoterPLacAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
FLAG-LoxP-PURO-LoxP-SNAP-TERT
Plasmid#71390PurposeHomologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes PURO resistance flanked by LoxP sites for selection.DepositorAvailable sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUC19-OCT4-T2A-NLS-EmGFP-P2A-Puro
Plasmid#89992PurposeDonor template for generation of OCT4-T2A-nEmGFP reporter cell linesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertOCT4-T2A-NLS-EmGFP-P2A-Puro (POU5F1 Human)
UseCRISPRPromoternoneAvailable sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONOR 6colors
Plasmid#229974PurposebigMamAct assembled plasmid for 6colors cellular labellingDepositorPublicationInsertmito-mCherry, EYFP-tubulin, H2B iRFP720, GTS-mTagBFP, mTFP1-actin
UseCRISPRExpressionMammalianMutationSee Depositor CommentsAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDonor_Tn[GFP]
Plasmid#213903PurposeMariner transposon expressing mNeonGreen with kan selection casetteDepositorInsertmariner transposon with kan resistance and mNeonGreen
ExpressionBacterialAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDonor_Tn[RFP]
Plasmid#213904PurposeMariner transposon expressing mCherry with cam selection casetteDepositorInsertmariner transposon with cam resistance and mCherry
ExpressionBacterialAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only