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pcDNA3.1-2xFLAG-2xSTREP-CDK6

Plasmid

#172614

Purpose

Expresses 2xFLAG-2xSTREP-tagged CDK6 in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CDK6 (CDK6 Human)

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-hCCND1-HA

Plasmid

#174154

Purpose

Lentiviral vector expressing HA-tagged human cyclin D1

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

CCND1 (CCND1 Human)

Use

Lentiviral

Tags

hemagglutinin (HA)

Expression

Mammalian

Promoter

EF1A

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_MAP3K8_WT

Plasmid

#82202

Purpose

Gateway Donor vector containing MAP3K8 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAP3K8 (MAP3K8 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_BAP1_WT

Plasmid

#81852

Purpose

Gateway Donor vector containing BAP1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

BAP1 (BAP1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-mVenus-p27K−

Plasmid

#176651

Purpose

It encodes an mVenus fused to a mutant p27K-, which lacks binding affinity to Cdk. This reporter helps to identify quiescent disseminated tumor cells (in G0 phase of cell cycle).

Depositor

Mohamed Bentires-Alj

Article

Correia et al Nature. 2021 Jun;594(7864):566-571. doi:

Insert

mVenus-p27K- (Cdkn1b Mouse)

Use

Lentiviral

Tags

mVenus

Mutation

Mutant K- (lacks affinity to Cdk)

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223_TGFBR1_WT

Plasmid

#82237

Purpose

Gateway Donor vector containing TGFBR1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TGFBR1 (TGFB1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RAC1_WT

Plasmid

#82157

Purpose

Gateway Donor vector containing RAC1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RAC1 (RAC1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RAC1_p.Q61L

Plasmid

#82178

Purpose

Gateway Donor vector containing RAC1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RAC1 (RAC1 Human)

Use

Gateway entry vector

Mutation

Q61L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RAC1_p.T17N

Plasmid

#82179

Purpose

Gateway Donor vector containing RAC1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RAC1 (RAC1 Human)

Use

Gateway entry vector

Mutation

T17N

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLL3.7 FLAG-YAP1-TEAD-P-H2B-mCherry

Plasmid

#128327

Purpose

Reporter to evaluate YAP1/TEAD-mediated gene transcription

Depositor

Yutaka Hata

Article

Maruyama et al Mol Cancer Res. 2018 Feb;16(2):197-211.

Inserts

H2B mCherry
YAP1 (YAP1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_BMP2_WT

Plasmid

#82101

Purpose

Gateway Donor vector containing BMP2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

BMP2 (BMP2 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ERN1_WT

Plasmid

#82115

Purpose

Gateway Donor vector containing ERN1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ERN1 (ERN1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CD79B_WT

Plasmid

#81750

Purpose

Gateway Donor vector containing CD79B , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CD79B (CD79B Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pJT300 Lenti pEF-dCas9-3XFLAG-ZNF705_KRAB-T2A-tagBFP-P2A-Blast

Plasmid

#195542

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

Lenti pEF-dCas9-3XFLAG-ZNF705_KRAB-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Promoter

pEF1a

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti HsATP13A2 WT

Plasmid

#171485

Purpose

transfer plasmid for lentiviral vector production expressing Hs ATP13A2 WT

Depositor

Veerle Baekelandt

Article

van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

Insert

human ATP13A2 (ATP13A2 Human)

Use

Lentiviral

Mutation

D508N , D962N

Promoter

CMV

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pDY0181 Cargo EGFP with AttP Bxb1 site (Parent minicircle)

Plasmid

#179115

Purpose

Cargo EGFP with AttP Bxb1 site (Parent minicircle)

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

Cargo EGFP with AttP Bxb1 site

Use

CRISPR

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223_STAT3_WT

Plasmid

#82235

Purpose

Gateway Donor vector containing STAT3, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

STAT3 (STAT3 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLX313-TP53-WT

Plasmid

#118014

Purpose

Constitutive expression of wild-type human Tumor Protein p53 (TP53)

Depositor

William Hahn, David Root

Article

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Insert

TP53 (TP53 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1alpha

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pDY0423 Cargo EGFP with AttP Bxb1 site for in-frame fusion (Parent minicircle)

Plasmid

#179116

Purpose

Cargo EGFP with AttP Bxb1 site for in-frame fusion (Parent minicircle)

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

Cargo EGFP with AttP Bxb1 site for in-frame fusion

Use

CRISPR

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223_DKK1_WT

Plasmid

#82250

Purpose

Gateway Donor vector containing DKK1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

DKK1 (DKK1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge