We narrowed to 23,036 results for: his

Showing: 10381 - 10400 of 23036 results

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pLei-sfGFP-D134*

Plasmid

#191110

Purpose

An E. coli sfGFP reporter with one TAG at position 134

Depositor

Han Xiao

Article

Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

Insert

superfolder green fluorescence protein

Tags

His tag

Expression

Bacterial

Mutation

changing D134 to TAG

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLei-sfGFP-V2*D134*

Plasmid

#191111

Purpose

An E. coli sfGFP reporter with two TAG at positions 2 & 134

Depositor

Han Xiao

Article

Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

Insert

superfolder green fluorescence protein

Tags

His tag

Expression

Bacterial

Mutation

changing V2 & D134 to TAG

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET-Cas9-FCPF

Plasmid

#220132

Purpose

Expresses Cas9-FCPF in E.coli

Depositor

Xinlai Cheng

Article

Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

Insert

Cas9-FCPF (cas9 S. pyogenes)

Tags

His and NLS

Expression

Bacterial

Promoter

T7

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pET50delCT_Vipp1_dL10Ala

Plasmid

#223817

Purpose

Bacterial expression of Vipp1_dL10Ala with C-terminal 6x His-Tag and 3C cleavage site

Depositor

Carsten Sachse

Article

Junglas et al Nat Struct Mol Biol. 2025 Mar;32(3):555-

Insert

Vipp1

Tags

6xHis

Expression

Bacterial

Mutation

replacement of 10 aa in Hinge 2 with Ala

Available Since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pET-28a (+)-Gal4-p53

Plasmid

#171076

Purpose

Expresses Gal4-p53 fusion protein in bacteria

Depositor

Joan Conaway

Article

He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

Insert

Gal4 DBD (NEWENTRY Budding Yeast)

Tags

His and p53(1-85)

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pColdI-GST-mGTSF2

Plasmid

#215714

Purpose

Bacterial expression of GST-tagged GTSF2

Depositor

Phillip Zamore

Article

Arif et al Nature. 2022 Aug;608(7923):618-625. doi:

Insert

GTSF2 (Gtsf2 Mouse)

Tags

6x His and GST

Expression

Bacterial

Promoter

CspA

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-jGCaMP8f L27A

Plasmid

#204140

Purpose

Fluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with L27A mutation

Depositor

Katalin Torok

Article

Tran et al Front Cell Neurosci. 2023 May 18;17:1155

Insert

jGCaMP8f L27A

Tags

N-terminal His tag

Expression

Mammalian

Mutation

Leucine 27 changed to Alanine

Promoter

CMV

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 R196E wgMDH

Plasmid

#204272

Purpose

Bacterial expression of R196E wgMDH with IPTG induction - Active site mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

R196E

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 R130Q wgMDH

Plasmid

#204268

Purpose

Bacterial expression of R130Q wgMDH with IPTG induction - Active site mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

R130Q

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 M66L wgMDH

Plasmid

#204247

Purpose

Bacterial expression of M66L wgMDH with IPTG induction - Subunit interface mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

M66L

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 M91Q wgMDH

Plasmid

#204250

Purpose

Bacterial expression of M91Q wgMDH with IPTG induction - Subunit interface mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

M91Q

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 D87L wgMDH

Plasmid

#204248

Purpose

Bacterial expression of D87L wgMDH with IPTG induction - Subunit interface mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

D87L

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-P19-NP

Plasmid

#196608

Purpose

siRNA vector expressing TMV P19 and a 425bp-long NP sequence as inverted repeat

Depositor

Franco Lucchini

Article

Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

Insert

p19 + Influenza virus NP inverted repeat

Tags

6x His tag

Expression

Bacterial

Available Since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL1936

Plasmid

#195692

Purpose

Complementation of MlaF-MlaE-MlaDdelta153-183-MlaC-MlaB

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-MlaE-MlaDdelta153-183-MlaC-MlaB

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL1935

Plasmid

#195693

Purpose

Complementation of MlaF-MlaE-MlaDdelta173-183-MlaC-MlaB

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-MlaE-MlaDdelta173-183-MlaC-MlaB

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL1953

Plasmid

#195694

Purpose

Complementation of MlaF-MlaE-MlaD(F118A/L123A)-MlaC-MlaB

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-MlaE-MlaD(F118A/L123A)-MlaC-MlaB

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL1933

Plasmid

#195695

Purpose

Complementation of MlaF-MlaE-MlaD(F118A)-MlaC-MlaB

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-MlaE-MlaD(F118A)-MlaC-MlaB

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL1934

Plasmid

#195696

Purpose

Complementation of MlaF-MlaE-MlaD(L123A)-MlaC-MlaB

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-MlaE-MlaD(L123A)-MlaC-MlaB

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL2766

Plasmid

#196028

Purpose

Complementation of MlaF-MlaE-MlaDdelta141-183-MlaC-MlaB

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-MlaE-MlaDdelta141-183-MlaC-MlaB

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pFR-mTq2-antiFRET-P2A-RCaMP1h

Plasmid

#191467

Purpose

Compare the intensity of the genetically encoded calcium sensor (GECI) RCaMP1h to mTurquoise2 fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al eLife 14:RP105086; doi: https://doi.org/

Insert

RCaMP1h

Tags

His-mTurquoise2-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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