We narrowed to 11,588 results for: Vars;

1. pAAV hSyn1 CaRhAC T2A tDimer

   Plasmid

   #101722

   Purpose

   - humanized - variant of pAAV hSyn YFP-CaRhAC Addgene # 101721– less reliable in hippocampal neurons

   Depositor

   Thomas Oertner

   Article

   Scheib et al Nat Commun. 2018 May 24;9(1):2046. doi:

   Inserts

   CatRhAC
   red fluorescent protein
   

   Use

   AAV

   Mutation

   E497K,C569D

   Promoter

   human Synapsin1 promotor and ribosomal skip seque…

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pnEA-NpM-HsNot4-GB1His6_AG

   Plasmid

   #148792

   Purpose

   Bacterial Expression of HsNot4

   Depositor

   Elisa Izaurralde

   Article

   Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

   Insert

   HsNot4 (CNOT4 Human)
   

   Expression

   Bacterial

   Mutation

   one silent mutation compared to the sequence give…

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCEP4-HA-Flag-natT1R2 a22-end_I132S-S212N

   Plasmid

   #113952

   Purpose

   mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

   Depositor

   Erik Procko

   Article

   Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

   Insert

   T1R2 (TAS1R2 Human)
   

   Tags

   FLAG and Signal/leader sequence from influenza he…

   Expression

   Mammalian

   Mutation

   delete N-terminal 21 residues, I132S and S212N su…

   Promoter

   cmv

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET19-M(BRCT)TdTwt

   Plasmid

   #207459

   Purpose

   Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) variant, including the N-terminal BRCT domain

   Depositor

   Jay Keasling

   Article

   Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

   Insert

   M(BRCT)TdTwt (Dntt Mouse)
   

   Tags

   10xHis-MBP

   Expression

   Bacterial

   Available Since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCEP4-HA-Flag-natT1R2 FS10

   Plasmid

   #113956

   Purpose

   mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

   Depositor

   Erik Procko

   Article

   Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

   Insert

   T1R2 (TAS1R2 Human)
   

   Tags

   FLAG and Signal/leader sequence from influenza he…

   Expression

   Mammalian

   Mutation

   delete N-terminal 21 residues, S212N substitution…

   Promoter

   cmv

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-iLMO4 (GLucM23-iChloC-EYFP)

   Plasmid

   #114102

   Purpose

   fusion protein of Gaussia luciferase variant M23, improved slowChloC anion conducting ChR, and enhanced yellow fluorescent protein for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Park et al J Neurosci Res. 2017 Sep 1. doi: 10.1002

   Insert

   GLucM23-iChloC-EYFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMT-Pav-GFP

   Plasmid

   #24286

   Depositor

   Ron Vale

   Article

   Vale et al J Cell Biol. 2009 Sep 7. 186(5):727-38.

   Insert

   Pavarotti (pav Fly)
   

   Tags

   GFP

   Expression

   Insect

   Available Since

   June 29, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pAAV-hSyn-LMO4 (GLucM23-VChR1-EYFP)

   Plasmid

   #114101

   Purpose

   fusion protein of Gaussia luciferase variant M23, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Park et al J Neurosci Res. 2017 Sep 1. doi: 10.1002

   Insert

   Gaussia luciferase, M23; Volvox Channelrhodopsin 1; EYFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA-Prom1 D-5

   Plasmid

   #184017

   Purpose

   Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of exon 19a (amino acids 696-701), and deletion of amino acids 641 to 655.

   Depositor

   Peter Stoilov

   Article

   Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

   Insert

   Prom1 SV8(-Ex19a) D-5 (Prom1 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Deletion of exon 19a (amino acids 696-701), and d…

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGG426_UV5

    Plasmid

    #165608

    Purpose

    Vector for expression of the SpCas9 VRKG variant with sgRNA in E. coli: lacUV5-VRKG(SpCas9, D1135V/S1136R/D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)

    Depositor

    Marcus Noyes

    Article

    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

    Insert

    lacUV5 driving Streptococcus pyogenes Cas9 VRKG(D1135V/S1136R/D1332K/R1333G) and hEGFP-sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Mutation

    D1135V, S1136R, D1332K and R1333G mutations in Sp…

    Promoter

    lacUV5 driving Cas9 VRKG and UV5 driving sgRNA

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hSyn-sbGLuc-CheRiff-EGFP

    Plasmid

    #114108

    Purpose

    fusion protein of Gaussia luciferase variant slow burn, synthetic channelrhodopsin CheRiff, and enhanced green fluorescent protein for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Ikefuama et al Int J Mol Sci. 2022 Oct 27;23(21):12994.

    Insert

    Gaussia luciferase, slow burn; CheRiff synthetic ChR; EGFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. lentiGuideFB-Puro-A

    Plasmid

    #192506

    Purpose

    For cloning of sgRNAs compatible with PspCas9. Contains a 5' direct repeat and a nd a unique sgRNA scaffold variant with a capture sequence. Cloning guide RNAs using BsmBI.

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Methods. 2023 Jan;20(1):86-94. doi:

    Insert

    hU6-sgRNA-CS1-BsmBI-EFS-Puro-WPRE
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCEP4-HA-Flag-natT1R2 a22-end_S212N

    Plasmid

    #113951

    Purpose

    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R2 (TAS1R2 Human)
    

    Tags

    FLAG and Signal/leader sequence from influenza he…

    Expression

    Mammalian

    Mutation

    delete N-terminal 21 residues, S212N substitution

    Promoter

    CMV

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. lentiGuideFB-Puro-B

    Plasmid

    #192507

    Purpose

    For cloning of sgRNAs compatible with PspCas9. Contains a 5' direct repeat and a unique sgRNA scaffold variant with a capture sequence. Cloning guide RNAs using BsmBI.

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Methods. 2023 Jan;20(1):86-94. doi:

    Insert

    bU6-sgRNACR2-CS-BsmBI-EFS-Puro-WPRE
    

    Use

    Lentiviral

    Promoter

    bU6

    Available Since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDual-eGFP(Stop66)

    Plasmid

    #63218

    Purpose

    Expression of a non-sense mutant version of eGFP (dark) in bacteria and in mammalian cells. Could be used as a reporter for gene targeting and recombineering in mammalian cells.

    Depositor

    Richard S Myers

    Article

    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

    Insert

    His-T7-eGFP(Stop66)
    

    Use

    Lentiviral

    Tags

    T7 and his6

    Expression

    Bacterial and Mammalian

    Mutation

    Changed tyrosine at position 66 with respect to t…

    Promoter

    CMV-EF1α hybrid (CEF)

    Available Since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

16. C-His-GvpC-TEV

    Plasmid

    #153296

    Purpose

    Expresses TEV-sensitive variant of Anabaena flos-aquae GvpC in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Insert

    TEV-sensitive Gas vesicle protein C (his-tagged)
    

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    1) 21 bp TEV recognition and cleavage site flanke…

    Promoter

    T7

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. N-His-GvpC-ssrA

    Plasmid

    #153297

    Purpose

    Expresses ClpXP-degradable variant of Anabaena flos-aquae GvpC in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Insert

    Gas vesicle protein C (with N-terminal his-tag and C-terminal ssrA tag)
    

    Tags

    His-tag and ssrA tag

    Expression

    Bacterial

    Mutation

    1) N-terminal 6xHis tag flanked by 1x glycine res…

    Promoter

    T7

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pVE2_3_p40S_QfDBD_VP16AD_cp_pcbC_DsRed_11xQUAS

    Plasmid

    #154223

    Purpose

    Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 11xQUAS+CP_pcbC

    Depositor

    Arnold Driessen

    Article

    Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.

    Insert

    p40S:QfDBD-VP16AD-GFP-NLS
    

    Use

    Synthetic Biology; Fungal expression

    Tags

    GFP-NLS; DsRed-SKL

    Expression

    Bacterial

    Promoter

    p40S (AN0465 A. nidulans)

    Available Since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLM2_42_pgndA_QfDBD_VP16AD_cp_pcbC_DsRed_5xQUAS

    Plasmid

    #154238

    Purpose

    Encodes pgndA:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS+CP_pcbC

    Depositor

    Arnold Driessen

    Article

    Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.

    Insert

    pgndA:QfDBD-VP16AD-GFP-NLS
    

    Use

    Synthetic Biology; Fungal expression

    Tags

    GFP-NLS; DsRed-SKL

    Expression

    Bacterial

    Promoter

    pGndA (An11g02040 A. niger)

    Available Since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZac2.1-GfaABC1D-mCherry-hPMCA2x/b

    Plasmid

    #111569

    Purpose

    Expresses a plasma membrane calcium pump hPMCA2x/b

    Depositor

    Baljit Khakh

    Article

    Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

    Insert

    Plasma membrane calcium pump 2, variant x/b (ATP2B2 Human)
    

    Tags

    mCherry

    Available Since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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