We narrowed to 11,662 results for: Kars

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pCold I-Hero9

Plasmid

#187927

Purpose

Bacterial expression of heat-resistant obscure (Hero) protein Hero9

Depositor

Yukihide Tomari

Article

Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

Insert

Hero9

Tags

Flag-6xHis

Expression

Bacterial

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pSG5-HA-H-Ras_wt

Plasmid

#39509

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS (HRAS Human)

Use

Eukaryotic

Tags

HA

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
SLC9A3R1_PDZ_2

Plasmid

#103933

Purpose

Protein expression and purification of SLC9A3R1 PDZ2 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

SLC9A3R2 (NHERF2 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP eIF2beta

Plasmid

#45902

Depositor

Louise Larose

Article

Kebache et al Proc Natl Acad Sci U S A. 2002 Apr 16;99

Insert

eIF2S2 (Eif2s2 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
pcold-6xHis-HRV3Csite-DmLoqsPA

Plasmid

#41093

Depositor

Phillip Zamore

Article

Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

Insert

Loquacious-PA (loqs Fly)

Tags

6xHis-HRV3Csite

Expression

Bacterial

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only
MigR1-Flag-TRIB2

Plasmid

#131154

Purpose

Retroviral expression of flag-tagged mouse TRIB2

Depositor

Karen Keeshan, Warren Pear

Article

Keeshan et al Cancer Cell. 2006 Nov;10(5):401-11. doi:

Insert

Trib2 (Trib2 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PKAsub-YPet-NES

Plasmid

#138203

Purpose

Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.

Depositor

Jin Zhang

Article

Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

Insert

PKAsub-YPet-NES

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

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NLS-dCas9-HA-NLS-GFP4-VPR

Plasmid

#183924

Purpose

VPR activation domain coupled to catalytically inactive dCas9 for localization; contains a GFP tetramer fpr labeling and as a spacer

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

dCas9-GFP4-VPR

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pOmpT

Plasmid

#112123

Purpose

Bacterial expression outer membrane protein T (OmpT), wild type

Depositor

Karen Fleming

Article

Burgess et al J Biol Chem. 2008 Sep 26;283(39):26748-5

Insert

OmpT (ompT E. coli)

Expression

Bacterial

Mutation

N-terminal signal sequence not included and repla…

Promoter

T7

Available Since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pOmpW

Plasmid

#112121

Purpose

Bacterial expression of outer membrane protein W (OmpW), wild type

Depositor

Karen Fleming

Article

Burgess et al J Biol Chem. 2008 Sep 26;283(39):26748-5

Insert

OmpW (ompW E. coli)

Expression

Bacterial

Mutation

N-terminal signal sequence not included and repla…

Promoter

T7

Available Since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pUE1-TSP-ORF68 K395A/K396A

Plasmid

#162656

Purpose

Expresses ORF68 K395A/K396A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II and HRV 3C site

Expression

Mammalian

Mutation

Amino acids K395 and K396 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcold-6xHis-HRV3Csite-DmLoqsPD

Plasmid

#41095

Depositor

Phillip Zamore

Article

Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

Insert

Loquacious-PD (loqs Fly)

Tags

6xHis-HRV3Csite

Expression

Bacterial

Available Since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only
pSG5-Myr-FLAG-p110β-DM

Plasmid

#55719

Purpose

eukaryotic expression of myristoylation tag (Myr-FLAG) p110b RBD double mutant S205D, K224A

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

p110 beta

Tags

myristoylation tag (Myr-FLAG)

Expression

Mammalian

Mutation

S205D, K224A

Promoter

T7

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pRVdG-4FLPo

Plasmid

#122050

Purpose

Genome plasmid for deletion-mutant rabies virus encoding Flpo recombinase

Depositor

Ian Wickersham

Article

Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

Insert

FLPo

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pcold-6xHis-HRV3Csite-DmLoqsPB

Plasmid

#41094

Depositor

Phillip Zamore

Article

Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

Insert

Loquacious-PB (loqs Fly)

Tags

6xHis-HRV3Csite

Expression

Bacterial

Available Since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only
ptagBFP-TetR-NLS-VP16

Plasmid

#103835

Purpose

Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited tetO arrays

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

tagBFP-TetR-NLS-VP16 (tetR )

Expression

Mammalian

Promoter

SV2

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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GST eIF2beta

Plasmid

#45900

Depositor

Louise Larose

Article

Kebache et al Proc Natl Acad Sci U S A. 2002 Apr 16;99

Insert

eIF2S2 (Eif2s2 Mouse)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
SLC9A3R2_PDZ_2

Plasmid

#103926

Purpose

Protein expression and purification of NHERF2 PDZ2 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

SLC9A3R2 (NHERF2 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pSV HA eIF2beta WT

Plasmid

#45898

Depositor

Louise Larose

Article

Kebache et al Proc Natl Acad Sci U S A. 2002 Apr 16;99

Insert

Eif2s2 (Eif2s2 Mouse)

Tags

HA

Expression

Mammalian

Promoter

SV40

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-RLuc8C-FHA1-NES

Plasmid

#138210

Purpose

Encodes PAABD fragment of luminescence-based bimolecular kinase activity reporters (LumKARs); cytosol targeted.

Depositor

Jin Zhang

Article

Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

Insert

RLuc8C-FHA1-NES

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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