We narrowed to 23,595 results for: flag

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pCMV-FLAG-PGRMC1-1-170

Plasmid

#173467

Purpose

PGRMC1 lacking the extreme C-terminus

Depositor

Bill Tsai

Article

Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

Insert

PGRMC1 (PGRMC1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Deleted for amino acids 171-194

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCI-Flag-NEDD4L∆WW4

Plasmid

#166710

Purpose

expresses NEDD4L with inactivating mutation in fourth WW domain in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

NEDD4L W576A

Tags

FLAG

Expression

Mammalian

Mutation

changed tryptophan 576 to alanine

Promoter

CMV promoter

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCI-Flag-NEDD4L∆WW1

Plasmid

#166707

Purpose

expresses NEDD4L with inactivating mutation in first WW domain in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

NEDD4L W221A

Tags

FLAG

Expression

Mammalian

Mutation

changed tryptophan 221 to alanine

Promoter

CMV promoter

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCI-Flag-NEDD4L∆WW1,2,4

Plasmid

#171137

Purpose

expresses NEDD4L with inactivating mutation in first, second and fourth WW domain, but intact third WW domain, in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

NEDD4L (NEDD4L Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed tryptophan 221 to alanine, tryptophan 453…

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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His-SUMO-flag-hDDI2_pET11a

Plasmid

#169121

Purpose

expression of protein

Depositor

Barbara Dirac-Svejstrup

Article

Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

Insert

hDDI (DDI2 Human)

Expression

Bacterial

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pET-FLAG-SpCas9-HF1

Plasmid

#126770

Purpose

Expression of increased fidelity SpCas9-HF1 in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

SpCas9-HF1

Use

CRISPR

Tags

3xFLAG, 6xHis, MBP, and NLS

Expression

Bacterial

Mutation

N497A, R661A, Q695A, Q926A

Promoter

T7

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-MBP1-E322TAG-W340A.pcDNA3-k

Plasmid

#127404

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site.

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid mutations: E322 to Amber codon and W34…

Promoter

CMV

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-M-Flag-BAP1-F170C

Plasmid

#125848

Purpose

express mutant BAP1-F170C

Depositor

Boyi Gan

Article

Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

Insert

BAP1 (BRCA1 associated protein 1) (BAP1 Human)

Use

Lentiviral

Tags

Flag

Mutation

F170C

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-M-Flag-BAP1-G109V

Plasmid

#125846

Purpose

express mutant BAP1-G109V

Depositor

Boyi Gan

Article

Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

Insert

BAP1 (BRCA1 associated protein 1) (BAP1 Human)

Use

Lentiviral

Tags

Flag

Mutation

G109V

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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Brm BDmut-Flag pShuttle

Plasmid

#122108

Purpose

Expression of full length Brm containing point mutations in its bromodomain in mammalian cells

Depositor

Paul Herring

Article

Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

Insert

Brm (SMARCA2 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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p3x-Flag Gli3 37R3-2

Plasmid

#120305

Purpose

Expresses human protein Gli containing a 4HT-inducible intein evolved to operate at 37C

Depositor

David Liu

Article

Peck et al Chem Biol. 2011 May 27;18(5):619-30. doi

Insert

Intein 37R3-2, Gli3

Use

Synthetic Biology

Tags

3X Flag

Expression

Mammalian

Mutation

Contains mutations as reported in 2011 Chemistry …

Promoter

CMV

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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p3x-Flag Gli3 30R3-1

Plasmid

#120304

Purpose

Expresses human protein Gli containing a 4HT-inducible intein evolved to operate at 30C

Depositor

David Liu

Article

Peck et al Chem Biol. 2011 May 27;18(5):619-30. doi

Insert

30R3-1, Gli3

Use

Synthetic Biology

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG Enthoprotin Full Length

Plasmid

#47419

Depositor

Peter McPherson

Article

Wasiak et al J Cell Biol. 2002 Sep 2;158(5):855-62. d

Insert

Enthoprotin

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only
MDH1-PGK-FLAG-TAT-86-A

Plasmid

#35977

Depositor

Susana Valente

Article

Mediouni et al Curr HIV Res. 2015;13(1):64-79.

Insert

transactivating protein (tat Human Immunodeficiency Virus)

Use

RNAi and Retroviral

Tags

Flag

Expression

Mammalian

Mutation

From the "A" HIV clade

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-SZT2 1-1021

Plasmid

#87036

Purpose

mammalian expression of SZT2 fragment

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

SZT2 (SZT2 Human)

Tags

Flag

Expression

Mammalian

Mutation

AA 1-1021

Promoter

CMV

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-Smad4 P130S

Plasmid

#80892

Purpose

mammalian expression of Smad4 P130S

Depositor

Aristidis Moustakas

Article

Moren et al Oncogene. 2000 Sep 7;19(38):4396-404.

Insert

SMAD4 (SMAD4 Human)

Tags

Flag

Expression

Mammalian

Mutation

colorectal cancer mutation P130S; additional D2E

Promoter

CMV

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pX330-Flag-HeFSpCas9-H840A

Plasmid

#80456

Purpose

high fidelity enhanced SpCas9 mammalian expression vector

Depositor

Ervin Welker

Insert

HeFSpCas9_H840A

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Promoter

CBh

Available Since

Aug. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG/MKRN1(1-280)

Plasmid

#78752

Purpose

To overexpress MKRN1(1-280) in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

Insert

MKRN1 (MKRN1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

1-280

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-RIPK1 K115R

Plasmid

#78843

Purpose

To overexpress RIPK1 K115R in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK1 (RIPK1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

K115R

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-Ago2-FDM5

Plasmid

#72212

Purpose

Mammalian expression of aa 518-860 of human Ago2

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

argonaute RISC catalytic component 2 (AGO2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Δ 1-517

Promoter

CMV

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only