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We narrowed to 15,013 results for: flag

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Showing: 10521 - 10540 of 15013 results

  2. FNL Combinatorial Cloning Platform

    Plasmid kit
    #1000000211
    Purpose
    A Frederick National Laboratory (FNL) combinatorial cloning set to generate many tagged expression clones in parallel for protein production, in vivo gene expression, and shRNA and CRISPR delivery.
    Depositor
    Dominic Esposito
    Application
    Cloning and Synthetic Biology, Gene Expression and Labeling
    Vector type
    Bacterial Expression, Insect Expression, Mammalian Expression
    Cloning type
    MultiSite Gateway
    Available since
    Dec. 22, 2022
    Availability
    Academic Institutions and Nonprofits only

  6. dCas9-p300 HAT

    Plasmid
    #179544
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT domain (aa 1287-1664) driven by AF4 promoter
    Depositor
    Isaac Hilton
    Publication
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human p300 HAT domain (aa 1287-1664) (EP300 Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179544-plasmid-map-sequence-id-354774

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  7. Negative Control Binder

    Plasmid
    #179140
    Purpose
    Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9) and an additionional A70Q mutation greatly reduces affinity for SsrA peptide
    Depositor
    Klaus Hahn
    Publication
    Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SspB
    Tags
    FLAG and mCherry
    Expression
    Mammalian
    Mutation
    Y11K/A15E, residue numbering from pdb: 1ou9. Addi…
    Available since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    179140-plasmid-map-sequence-id-353410

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  10. LV-AR-KKKAAA

    Plasmid
    #171219
    Purpose
    Lenti-viral expression of human androgen receptor DNA-binding mutant: K630A-K632A-K633A, mutations at the boundary between AR DNA-binding domain and hinge domain.
    Depositor
    Steven Balk
    Publication
    Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:
    Insert
    AR-KKKAAA (AR Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    AR-K630A-K632A-K633A
    Promoter
    EF-1a
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    171219-plasmid-map-sequence-id-342535

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  11. pGWB601NL3F10H

    Plasmid
    #141287
    Purpose
    No promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, BASTA R for plant)
    Depositor
    Andrew Millar
    Publication
    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10
    Type
    Empty backbone
    Use
    Gateway Cloning and Luciferase
    Tags
    FLAG (x3), HIS (x10), and NLUC
    Expression
    Plant
    Available since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    141287-plasmid-map-sequence-id-338006

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  12. LV-AR-C619Y

    Plasmid
    #171222
    Purpose
    Lenti-viral expression of human androgen receptor DNA-binding mutant: C619Y, mutation at the end of zinc finger 2.
    Depositor
    Steven Balk
    Publication
    Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:
    Insert
    AR-C619Y (AR Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    AR-C619Y
    Promoter
    EF-1a
    Available since
    July 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    171222-plasmid-map-sequence-id-336120

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  14. pCMV-YdLight1

    Plasmid
    #125705
    Purpose
    Expresses the yellow genetically encoded dopamine sensor YdLight1 in mammalian cells.
    Depositor
    Lin Tian
    Publication
    Patriarchi et al Nat Methods. 2020 Nov;17(11):1147-1155.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    YdLight1
    Tags
    Flag tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    125705-plasmid-map-sequence-id-246162

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  15. FKBP-CD28TMD-NTEVp(WT)

    Plasmid
    #137826
    Purpose
    MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal WT TEV protease
    Depositor
    Joshua Leonard
    Publication
    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FKBP-CD28TMD-NTEVp(WT)
    Tags
    3x FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    137826-plasmid-map-sequence-id-311921

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  18. HsSPHK1

    Plasmid
    #118091
    Purpose
    Expresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)
    Depositor
    Kevin Lynch
    Publication
    Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do
    Insert
    Homo sapiens Sphingosine Kinase 1
    Tags
    FLAG
    Expression
    Yeast
    Promoter
    GAL1, 10
    Available since
    Aug. 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    118091-plasmid-map-sequence-id-234124

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  19. MmSphk1

    Plasmid
    #118599
    Purpose
    Expresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate in mice
    Depositor
    Kevin Lynch
    Publication
    Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do
    Insert
    Mus musculus sphingosine kinase 1
    Tags
    FLAG
    Expression
    Yeast
    Promoter
    GAL1, 10
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    118599-plasmid-map-sequence-id-234126

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  20. pEBTet-SNAP-Centrobin

    Plasmid
    #136829
    Purpose
    Mammalian expression of the centrosomal protein Centrombin N-terminally fused to SNAP-tag
    Depositor
    Kai Johnsson
    Publication
    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SNAP-Centrobin (CNTROB Human, Synthetic)
    Tags
    FLAG-tag / His-tag
    Expression
    Mammalian
    Promoter
    CMV-TetO2
    Available since
    March 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    136829-plasmid-map-sequence-id-276002

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Showing: 10521 - 10540 of 15013 results