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Showing: 1561 - 1590 of 8890 results

  1. pTWIN1-His6-Ssp-Htt2-90(A2C)-15Q-P90C

    Plasmid
    #114610
    Purpose
    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P90C cysteine mutations and a 15 polyQ repeat
    Depositor
    Hilal Lashuel
    Article
    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Use
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    A2C, P90C; 15 polyQ repeat
    Promoter
    Available since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    114610-plasmid-map-sequence-id-222711

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  2. pTWIN1-His6-Ssp-Htt2-90(A2C)-15Q-P70C

    Plasmid
    #114609
    Purpose
    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P70C cysteine mutations and a 15 polyQ repeat
    Depositor
    Hilal Lashuel
    Article
    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Use
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    A2C, P70C; 15 polyQ repeat
    Promoter
    Available since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    114609-plasmid-map-sequence-id-222709

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  3. pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q-P90C

    Plasmid
    #114620
    Purpose
    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P90C cysteine mutations and a 37 polyQ repeat
    Depositor
    Hilal Lashuel
    Article
    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Use
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    A2C, P90C; 37 polyQ repeat
    Promoter
    Available since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    114620-plasmid-map-sequence-id-222661

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  4. pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q-P80C

    Plasmid
    #114619
    Purpose
    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P80C cysteine mutations and a 37 polyQ repeat
    Depositor
    Hilal Lashuel
    Article
    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Use
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    A2C, P80C; 37 polyQ repeat
    Promoter
    Available since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    114619-plasmid-map-sequence-id-222659

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  5. pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q-A60C

    Plasmid
    #114618
    Purpose
    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & A60C cysteine mutations and a 37 polyQ repeat
    Depositor
    Hilal Lashuel
    Article
    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Use
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    A2C, A60C; 37 polyQ repeat
    Promoter
    Available since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    114618-plasmid-map-sequence-id-222655

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  6. pTWIN1-His6-Ssp-Htt2-90(A2C)-15Q-A60C

    Plasmid
    #114608
    Purpose
    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & A60C cysteine mutations and a 15 polyQ repeat
    Depositor
    Hilal Lashuel
    Article
    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Use
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    A2C, A60C; 15 polyQ repeat
    Promoter
    Available since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    114608-plasmid-map-sequence-id-222653

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  8. pET21b(+)-Is-PETase-W159H-S238F

    Plasmid
    #112203
    Purpose
    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W159H and S238F mutations, codon optimized for expression in E. coli K12
    Depositor
    Christopher Johnson, Gregg Beckham
    Article
    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115
    Insert
    PETase gene from Ideonella sakaiensis 201-F6 with W159H and S238F mutations, codon optimized for expression in E. coli K12
    Use
    Tags
    6XHIS
    Expression
    Bacterial
    Mutation
    W159H, S238F
    Promoter
    N/A
    Available since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    112203-plasmid-map-sequence-id-215852

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  10. pEN487 - Sororin-AID[71-114]-eGFP-FRT-Blast-FRT targeting conuct

    Plasmid
    #156433
    Purpose
    Targeting vector to introduce an AID-eGFP cassette at the mouse Cdca5 (SORORIN) locus using BLASTICIDIN selection. Auxin-inducible degron system. Designed to be used with sgRNA GGGATGCCCGTCATTAAGTG
    Depositor
    Elphege Nora
    Article
    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:
    Insert
    SORORIN, AID (Cdca5 Mouse)
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    none
    Promoter
    Available since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    156433-plasmid-map-sequence-id-310157

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  11. pTWIN1-His6-Ssp-Htt18-90(Q18C)-42Q-P90C

    Plasmid
    #114606
    Purpose
    Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & P90C cysteine mutations and a 42 polyQ repeat
    Depositor
    Hilal Lashuel
    Article
    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Use
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    dNt17 (deletion of first 17 amino acids), Q18C, P…
    Promoter
    Available since
    Oct. 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    114606-plasmid-map-sequence-id-225647

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  12. pTWIN1-His6-Ssp-Htt18-90(Q18C)-22Q-P80C

    Plasmid
    #114602
    Purpose
    Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & P80C cysteine mutations and a 22 polyQ repeat
    Depositor
    Hilal Lashuel
    Article
    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Use
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    dNt17 (deletion of first 17 amino acids), Q18C, P…
    Promoter
    Available since
    Oct. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114602-plasmid-map-sequence-id-224743

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  13. pTWIN1-His6-Ssp-Htt18-90(Q18C)-22Q-A60C

    Plasmid
    #114601
    Purpose
    Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & A60C cysteine mutations and a 22 polyQ repeat
    Depositor
    Hilal Lashuel
    Article
    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Use
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    dNt17 (deletion of first 17 amino acids), Q18C, A…
    Promoter
    Available since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    114601-plasmid-map-sequence-id-222795

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  14. pTWIN1-His6-Ssp-Htt18-90(Q18C)-42Q-P70C

    Plasmid
    #114605
    Purpose
    Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & P70C cysteine mutations and a 42 polyQ repeat
    Depositor
    Hilal Lashuel
    Article
    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Use
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    dNt17 (deletion of first 17 amino acids), Q18C, P…
    Promoter
    Available since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    114605-plasmid-map-sequence-id-222758

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  15. pTWIN1-His6-Ssp-Htt18-90(Q18C)-42Q-A60C

    Plasmid
    #114604
    Purpose
    Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & A60C cysteine mutations and a 42 polyQ repeat
    Depositor
    Hilal Lashuel
    Article
    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Use
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    dNt17 (deletion of first 17 amino acids), Q18C, A…
    Promoter
    Available since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    114604-plasmid-map-sequence-id-222736

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  16. pTWIN1-His6-Ssp-Htt18-90(Q18C)-22Q-P90C

    Plasmid
    #114603
    Purpose
    Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & P90C cysteine mutations and a 22 polyQ repeat
    Depositor
    Hilal Lashuel
    Article
    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Use
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    dNt17 (deletion of first 17 amino acids), Q18C, P…
    Promoter
    Available since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    114603-plasmid-map-sequence-id-222733

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  17. pAU003 - pTRE3G-Cterm_Nterm_switch_CTCF-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

    Plasmid
    #156430
    Purpose
    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.
    Depositor
    Elphege Nora
    Article
    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:
    Insert
    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Cterm_Nterm_switched
    Promoter
    Available since
    Sept. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    156430-plasmid-map-sequence-id-310203

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  18. pEN515 - pTRE3G-CTCF(ZFmut)-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

    Plasmid
    #156434
    Purpose
    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA (all Zinc fingers point mutated), targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA.
    Depositor
    Elphege Nora
    Article
    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:
    Insert
    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    All zinc fingers mutated
    Promoter
    Available since
    Sept. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    156434-plasmid-map-sequence-id-310270

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  20. pAU002 - pTRE3G-CTCF(DELTA1-265)-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

    Plasmid
    #156429
    Purpose
    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.
    Depositor
    Elphege Nora
    Article
    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:
    Insert
    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    DELTA1-265
    Promoter
    Available since
    Sept. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    156429-plasmid-map-sequence-id-310209

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  21. pEN366 - pTRE3G-CTCF-mRuby2-BGHpA-CAGGS-rtta3G-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE donor

    Plasmid
    #156432
    Purpose
    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible wild-type CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.
    Depositor
    Elphege Nora
    Article
    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:
    Insert
    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    none
    Promoter
    Available since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    156432-plasmid-map-sequence-id-310156

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  25. pcDNA3.1_ spike_del19

    Plasmid
    #155297
    Purpose
    Expression of spike with C-term deletion of 19 aa, used to generate high efficiency SARS-CoV-2-pseudotyped lentiviral particles
    Depositor
    Raffaele De Francesco
    Article
    Conforti et al Mol Ther. 2021 Sep 20. pii: S1525-0016(2
    Insert
    SARS-Cov2 spike_deleted (S SARS-Cov2)
    Use
    Generation of sars-cov-2-pseudotyped lentiviral p…
    Tags
    Expression
    Mutation
    Deletion in C-term 19 aa of spike
    Promoter
    CMV
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    155297-plasmid-map-sequence-id-307858

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  26. pHIPH4

    Plasmid
    #117685
    Purpose
    Hansenula polymorpha expression plasmid
    Depositor
    Ida van der Klei
    Insert
    Alcohol Oxidase promoter
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Alcohol Oxidase
    Available since
    Jan. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    117685-plasmid-map-sequence-id-232223

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  27. pCAS

    Plasmid
    #60847
    Purpose
    Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast
    Depositor
    Jamie Cate
    Article
    Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife
    Inserts
    S. pyogenese Cas9
    RNA pol III promoter (tRNA-Tyr)
    hepatitis delta virus ribozyme, genomic
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    NLS/His8 and TTT 3' extension prior to sgRNA
    Expression
    Bacterial and Yeast
    Mutation
    L4 is UUCG tetraloop and guide targets LYP1 (CATA…
    Promoter
    Available since
    Jan. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    60847-plasmid-map-sequence-id-197511

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  29. pLKO.1-TRC.mKO2_shB3GNT5.1

    Plasmid
    #110325
    Purpose
    TRCN0000034809 (Target GCCTATGTAATCTCCGGTGAT), silence human B3GNT5 gene and express monomeric Kusabira-Orange2
    Depositor
    Sandra Martha Gomes Dias
    Article
    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1
    Insert
    B3GNT5 (B3GNT5 Human)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    RNA polymerase III promoter for human U6 snRNA fo…
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    110325-plasmid-map-sequence-id-213398

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Showing: 1561 - 1590 of 8890 results