We narrowed to 18,992 results for: Met;

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pAND_Int2

Plasmid

#60590

Purpose

A transcriptional AND circuit constructed by layering NOR and NOT gates

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

AND_Int2

Expression

Bacterial

Promoter

PPhlF

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pSW104

Plasmid

#122295

Purpose

For run-off transcription of yeast RPL41A mRNA with and without an 82 nucleotide poly(A) tail.

Depositor

Sarah Walker

Article

Liu et al Methods. 2019 Mar 26. pii: S1046-2023(18

Insert

RPL41A

Use

In vitro transcription

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAP758-1

Plasmid

#99494

Purpose

TEV::eGFP::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::eGFP::3XFlag

Expression

Bacterial

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pAP546-2

Plasmid

#99476

Purpose

3XFlag::mNeptune::Myc

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::mNeptune::Myc

Expression

Bacterial

Available Since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pAND_yfp

Plasmid

#60592

Purpose

used with pSpec to generate an AND gate without memory

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

AND_yfp

Expression

Bacterial

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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PM-TD!TB

Plasmid

#48656

Purpose

Bacterial TD crRNA expression: targets TD to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial TD crRNA to prototspacer B

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pGH314_wtGFP S65T, Q80H

Plasmid

#85407

Purpose

lentiviral expression vector for wtGFP S65T-Q80H

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

wtGFP S65T, Q80H

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBPHLWL-QF

Plasmid

#26269

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

QF (qf Neurospora crassa)

Use

Drosophila transgenesis

Expression

Bacterial and Insect

Available Since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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FISSEQ-hgRNA-Design2

Plasmid

#100572

Purpose

FISSEQ-hgRNA-Design2 in a lentiviral backbone

Depositor

George Church

Article

Kalhor et al Nat Methods. 2017 Feb;14(2):195-200. doi

Insert

FISSEQ-hgRNA-Design2

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pSpec

Plasmid

#60593

Purpose

used with pAND_YFP to generate an AND gate without memory

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Spec

Expression

Bacterial

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pEB2-2ndVal mRFP1

Plasmid

#104025

Purpose

Plasmid encoding 2ndVal mRFP1

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal mRFP1

Use

Low copy

Expression

Bacterial

Mutation

mRFP1 with valine inserted in 2nd position

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCC48

Plasmid

#128285

Purpose

asymmetric plasmid partitioning E. coli - target plasmid

Depositor

Matthew Bennett

Article

Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

Insert

Piq - mRFP, BBa_J23100 - phlF, parS

Expression

Bacterial

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pAC-15-LexABD-G4MD-QFAD

Plasmid

#46105

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

LexABD-G4MD-QFAD

Expression

Insect

Available Since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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SK-YFP-TD-B

Plasmid

#48663

Purpose

Bacterial TD repression YFP reporter: protospacer B

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

TD prototspacer B/PAM with reporter EYFP

Use

CRISPR

Promoter

lambda pR promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pCis_7+8+10

Plasmid

#60588

Purpose

a 3-integrase plasmid

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Cis_7+8+10

Expression

Bacterial

Promoter

pBAD

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCis_7+8

Plasmid

#60587

Purpose

a 2-integrase plasmid

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Cis_7+8

Expression

Bacterial

Promoter

pBAD

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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EMX1_sgRNA

Plasmid

#100555

Purpose

Expresses EMX1 sgRNA. Target sequence: GAGTCCGAGCAGAAGAAGAA

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

EMX1 sgRNA

Promoter

U6

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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PM-NM!TA

Plasmid

#48651

Purpose

Bacterial NM crRNA expression: targets NM to protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial NM crRNA to prototspacer A

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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Phamret/pcDNA3

Plasmid

#51953

Purpose

mammalian expression of Phamret, a monomeric photoconvertible fluorescent protein.

Depositor

Takeharu Nagai

Article

Matsuda et al Nat Methods. 2008 Apr;5(4):339-45. doi:

Insert

Phamret

Expression

Mammalian

Available Since

March 18, 2014

Availability

Academic Institutions and Nonprofits only
pAP998-5

Plasmid

#99501

Purpose

3XFlag::tagBFP::linker::TEV

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::tagBFP::linker::TEV

Expression

Bacterial

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pAP683-2

Plasmid

#99488

Purpose

TEV::eGFP::ER::HA::linker::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::eGFP::RE::HA::linker::3XFlag

Expression

Bacterial

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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PM-TD!TA

Plasmid

#48655

Purpose

Bacterial TD crRNA expression: targets TD to protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial TD crRNA to prototspacer A

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pCis_2+5

Plasmid

#60586

Purpose

a 2-integrase plasmid

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Cis_2+5

Expression

Bacterial

Promoter

pBAD

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pEB2-2ndVal mNept2.5

Plasmid

#104032

Purpose

Plasmid encoding 2ndVal mNeptune2.5

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal mNeptune2.5

Use

Low copy

Expression

Bacterial

Mutation

mNeptune2.5 with valine inserted in 2nd position

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PM-NM!TB

Plasmid

#48652

Purpose

Bacterial NM crRNA expression: targets NM to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial NM crRNA to prototspacer B

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pattB-synaptobrevin-5-G4BDMD-QFADM1-hsp70

Plasmid

#46113

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

G4BDMD-QFADM1

Expression

Insect

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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PM-SP!TA

Plasmid

#48649

Purpose

Bacterial SP crRNA expression: targets SP to protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial SP crRNA to prototspacer A

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
FISSEQ-hgRNA-Design1

Plasmid

#100571

Purpose

FISSEQ-hgRNA-Design1 in a lentiviral backbone

Depositor

George Church

Article

Kalhor et al Nat Methods. 2017 Feb;14(2):195-200. doi

Insert

FISSEQ-hgRNA-Design1

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pAP576-2

Plasmid

#99481

Purpose

3XFlag::tagBFP::Myc

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::tagBFP::Myc

Expression

Bacterial

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pDS297

Plasmid

#34986

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

cpPDZ

Expression

Yeast

Promoter

GAL1

Available Since

March 21, 2012

Availability

Academic Institutions and Nonprofits only

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pattB-synaptobrevin-4-QFBDMD-G4AD-hsp70

Plasmid

#46112

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

QFBDMD-G4AD

Expression

Insect

Available Since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pattB-synaptobrevin-2-G4BDMD-QFAD-hsp70

Plasmid

#46110

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

G4BDMD-QFAD

Expression

Insect

Available Since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pattB-synaptobrevin-6-G4BDMD-QFADM2-hsp70

Plasmid

#46114

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

G4BDMD-QFADM2

Expression

Insect

Available Since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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YNP3N

Plasmid

#245858

Purpose

Archael (Methanonezhaarchaeon YNP3N) 16S rRNA expression

Depositor

Roland Hatzenpichler

Article

Van Beek et al Microbiol Resour Announc. 2025 October.

Insert

16S rRNA

Use

Other

Available Since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pNQ055

Plasmid

#242077

Purpose

Expresses MetRS (truncated at K548) as fusion with 6xHis-SUMO

Depositor

Olivier Duss

Article

Qureshi et al Nature. 2025 Jan;637(8045):487-495. doi:

Insert

metG (metG E. coli)

Tags

SUMO

Expression

Bacterial

Mutation

contains M1-K548

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pYL116

Plasmid

#239967

Purpose

CRISPR donor plasmid with Puro-T2A-mCherry cassette for Suz12 disruption

Depositor

Thomas Cech

Article

Long et al Methods. 2021 Jul;191:44-58. doi: 10.101

Insert

PuroR-T2A-mCherry with homology arms for human Suz12

Use

CRISPR

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pYL117

Plasmid

#239968

Purpose

CRISPR donor plasmid with Puro-T2A-mCherry cassette for EZH2 disruption

Depositor

Thomas Cech

Article

Long et al Methods. 2021 Jul;191:44-58. doi: 10.101

Insert

PuroR-T2A-mCherry with homology arms for human Ezh2

Use

CRISPR

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1551

Plasmid

#226498

Purpose

pMOBK360_StandVKL: Standard Vector (Kanamycin R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1552

Plasmid

#226499

Purpose

pMOBC360_StandVC: Standard Vector (Chloramphenicol R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
ExRai-PKNAR

Plasmid

#226631

Purpose

Excitation-ratiometric biosensor for monitoring Protein Kinase Novel activity in living cells

Depositor

Ju Chen

Article

Bogomolovas et al Commun Biol. 2025 Jan 22;8(1):109. doi:

Insert

ExRai-PKNAR

Expression

Mammalian

Available Since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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