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pGL3-24p3/LCN2-Luc

Plasmid

#25463

Depositor

Sarah Gaffen

Article

Shen et al J Leukoc Biol. 2005 Mar . 77(3):388-99.

Insert

24p3/lipocalin 2 proximal promoter (Lcn2 Mouse)

Use

Luciferase

Mutation

none

Available Since

June 25, 2010

Availability

Academic Institutions and Nonprofits only
pETM6-At4CL-PhCHS-CmCHI (C5 mutant, pFlavo-opt)

Plasmid

#73404

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

At4CL
PhCHS
CmCHI

Expression

Bacterial

Promoter

T7

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pDestattB_ubi:EGFP (pCM272)

Plasmid

#68339

Purpose

Multisite Gateway destination vector for phiC31-mediated transgenesis in zebrafish, harbors ubiquitin promoter (ubi)-driven EGFP.

Depositor

Christian Mosimann, Leonard Zon

Article

Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

Insert

EGFP

Use

Zebrafish transgenesis

Promoter

ubi

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pDisplay-UDP1.0-IRES-mCherry-CAAX

Plasmid

#220850

Purpose

CMV promoter drives the expression of an extracellular UDP sensor (UDP1.0) and a membrane localized mCherry

Depositor

Yulong Li

Article

Umpierre et al Neuron. 2024 Apr 10:S0896-6273(24)00195-

Insert

UDP1.0

Expression

Mammalian

Promoter

CMV

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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PresentER-MSIIFFLPL (GFP)

Plasmid

#102946

Purpose

PresentER minigene encoding H2-Kb ligand MSIIFFLPL (GFP)

Depositor

David Scheinberg

Article

Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

Insert

MSIIFFLPL (Serpinf1 Mouse, Synthetic)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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MSCVhygro-F-Ezh2-F667I

Plasmid

#24927

Depositor

Kai Ge

Article

Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

Insert

Ezh2 (EZH2 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Phenylanaline(F)667 mutated to Isoleucine(I)

Available Since

July 15, 2010

Availability

Academic Institutions and Nonprofits only
pEC3006 (pSpy1C-PZn_ffluc)

Plasmid

#218515

Purpose

replicative E. coli-S. pyogenes shuttle plasmid for zinc-inducible expression of the Firefly luciferase in S. pyogenes

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

ffluc

Use

Luciferase

Expression

Bacterial

Mutation

deletion of Plac_mrfp cassette

Promoter

PZn (PczcD)

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pRN3P-HA-Suv4-20h2

Plasmid

#86691

Purpose

For transcription of of Suv4-20h2 mRNA preceded by an HA-tag in 5'

Depositor

Maria-Elena Torres-Padilla

Article

Eid et al Genes Dev. 2016 Nov 15;30(22):2513-2526.

Insert

histone-lysine N-methyltransferase KMT5C (Kmt5c Mouse)

Tags

HA

Promoter

T3

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pU6-SAG1-DHFR

Plasmid

#80322

Purpose

Encodes a CRISPR sgRNA that allows for mutation of the surface antigen SAG1 in Toxoplasma gondii and confers resistance to pyrimethamine

Depositor

Sebastian Lourido

Article

Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

Insert

SAG1 sgRNA

Use

CRISPR

Promoter

U6

Available Since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF13B

Plasmid

#134626

Purpose

Mammalian expression of mouse KIF13B 442-1843

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF13B (Kif13b Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

WT

Promoter

Chicken Beta actin

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/V5 hsa-mir-150

Plasmid

#26310

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

hsa-mir-150 (MIR150 Human)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/V5 mmu-mir-133a-1

Plasmid

#26326

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-133a-1 (Mir133a-1 Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
MSCV eIF4E S209D IRES GFP

Plasmid

#18765

Depositor

Scott Lowe

Article

Wendel et al Genes Dev. 2007 Dec 15. 21(24):3232-7.

Insert

eIF4E S209D (Eif4e Mouse)

Use

Retroviral

Tags

IRES GFP

Expression

Mammalian

Mutation

S209D

Available Since

July 27, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 hsa-mir-124

Plasmid

#26306

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

hsa-mir-124 (MIR124-1 Human)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pCRISPRai

Plasmid

#189938

Purpose

Expresses CRISPRai cassette under EF1a promoter

Depositor

Yu-Chen Hu

Article

Truong et al Nucleic Acids Res. 2019 Jul 26;47(13):e7

Insert

CRISPRai cassette

Expression

Mammalian

Promoter

CMV enhancer-rat EF1 alpha

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-Decoy

Plasmid

#80324

Purpose

Encodes Cas9 and a CRISPR sgRNA that alleviates toxicity to Cas9 in Toxoplasma gondii

Depositor

Sebastian Lourido

Article

Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

Insert

Decoy sgRNA

Use

CRISPR

Promoter

U6

Available Since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pMIG-N19RhoA

Plasmid

#14120

Depositor

Richard Hynes

Article

Clark et al Nature. 2000 Aug 3. 406(6795):532-5.

Insert

N19 Human RhoA (RHOA Human)

Use

Retroviral

Tags

Myc

Mutation

Site directed mutagenesis of Thr to Asn at Codon …

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 hsa-mir-9-1

Plasmid

#26317

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

hsa-mir-9-1 (MIR9-1 Human)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/V5 hsa-mir-205

Plasmid

#26312

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

hsa-mir-205 (MIR205 Human)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
MSCV eIF4E W73A IRES GFP

Plasmid

#18762

Depositor

Scott Lowe

Article

Wendel et al Genes Dev. 2007 Dec 15. 21(24):3232-7.

Insert

eIF4E W73A (Eif4e Mouse)

Use

Retroviral

Tags

IRES GFP

Expression

Mammalian

Mutation

W73A

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
pCW57.1-pDUXCca

Plasmid

#198236

Purpose

Lentivector with tet-inducible porcine DUXC (codon altered)

Depositor

Stephen Tapscott

Article

Nip et al Hum Mol Genet. 2023 Feb 2:ddad021. doi:

Insert

porcine DUXC (codon altered)

Use

Lentiviral

Expression

Mammalian

Mutation

codon altered

Promoter

TRE

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/V5 hsa-mir-192

Plasmid

#26311

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

hsa-mir-192 (MIR192 Human)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pJL1-MBP-4xDQNAT

Plasmid

#128390

Purpose

In vitro expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Maltose binding protein

Tags

4xDQNAT glycosylation tag and 6xHis tag

Expression

Bacterial

Mutation

A302T- please see depositor comment

Promoter

T7

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/V5 hsa-mir-125

Plasmid

#26307

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

hsa-mir-125 (MIR125A Human)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 mmu-mir-223

Plasmid

#26334

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-223 (Mir223 Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
MSCV eIF4E E103A IRES GFP

Plasmid

#18766

Depositor

Scott Lowe

Article

Wendel et al Genes Dev. 2007 Dec 15. 21(24):3232-7.

Insert

eIF4E E103A (Eif4e Mouse)

Use

Retroviral

Tags

IRES GFP

Expression

Mammalian

Mutation

E103A

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 hsa-mir-214

Plasmid

#26313

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

hsa-mir-214 (MIR214 Human)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pHA-ΔN335-Kin1A

Plasmid

#31606

Depositor

Anthony Brown

Article

Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-50

Insert

ΔN335 kinesin-1A (Kif5a Mouse)

Tags

HA-tag

Expression

Mammalian

Mutation

deleted amino acids 1-335

Promoter

CMV

Available Since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 hsa-mir-142

Plasmid

#26309

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

hsa-mir-142 (MIR142 Human)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 mmu-mir-137

Plasmid

#26327

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-137 (Mir137 Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pAYC

Plasmid

#62657

Purpose

Mammalian expression of iCre recombinase from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Insert

iCre

Expression

Mammalian

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA3 14 mut B-Myb

Plasmid

#25967

Depositor

Rob Lewis

Article

Johnson et al J Biol Chem. 2002 Feb 8. 277(6):4088-97.

Insert

B-Myb (Mybl2 Mouse)

Tags

Flag

Expression

Bacterial

Mutation

Changed threonines 267, 408, 443, 447 490, 497, 5…

Available Since

Sept. 20, 2010

Availability

Academic Institutions and Nonprofits only
pDONR221-rap1-K9R

Plasmid

#84527

Purpose

This plasmid can be used as a donor for subcloning into Gateway destination vectors.

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Gateway donor plasmid

Mutation

K(43,61,69,117,240,246,527,582,651)R

Promoter

none

Available Since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 hsa-mir-128

Plasmid

#26308

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

hsa-mir-128 (MIR128-1 Human)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pXGFPC-5 Pxyl-dcas9 (Spy)

Plasmid

#133316

Purpose

expresses dcas9 from Streptococcus pyogenes; repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistance

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

dcas9 (Streptococcus pyogenes)

Use

CRISPR

Promoter

xylose

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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WZLneo-PPARγ2

Plasmid

#34566

Depositor

Kai Ge

Article

Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

Insert

PPARγ2 (Pparg Mouse)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

5'LTR

Available Since

Jan. 25, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 hsa-mir-193b

Plasmid

#26318

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

hsa-mir-193b (MIR193B Human)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pJL1-EPA-DNNNS-DQNRT

Plasmid

#128397

Purpose

In vitro expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Exotoxin A

Tags

6xHis tag

Expression

Bacterial

Mutation

242DNNNS246 and 384DQNRT388 engineered glycosylat…

Promoter

T7

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR4_FLAG/HA_FUS_His6

Plasmid

#26365

Depositor

Thomas Tuschl

Article

Spitzer et al Genes Chromosomes Cancer. 2011 May . 50(

Insert

FUS (FUS Human)

Use

Entry vector

Tags

FLAG/HA and His6

Mutation

delta 527 (stop codon)

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
pBCV

Plasmid

#62659

Purpose

Mammalian expression of FLPo recombinase from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Insert

FLPo

Expression

Mammalian

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge

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