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AOI-WT-Cas9-sg-mouse Ezh2-E10-GFP

Plasmid

#91879

Purpose

Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Ezh2

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

gRNA targeting Ezh2 (Ezh2 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only
AOI-WT-Cas9-sg-mouse Ezh2-E18-GFP

Plasmid

#91880

Purpose

Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Ezh2

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

gRNA targeting Ezh2 (Ezh2 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only
GP1BB

Plasmid

#51908

Purpose

Expresses full-length Platelet glycoprotein Ib beta chain precursor ectodomain in mammalian cells. Stop codon before C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

GP1BB (GP1BB Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Mutation

Stop Codon at amino acid 149, before CD4, bio and…

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_ NLS~K682A_K686A_K694_K709A (4KA) POLH

Plasmid

#246406

Purpose

Expresses K682A_K686A_K694_K709A POLH with an N-terminal NLS~FLAG tag in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

FLAG

Expression

Mammalian

Mutation

POLH K682A_K686A_K694_K709A. Coding sequence has …

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_ NLS~K682R_K686R_K69R_K709R (4KR) POLH

Plasmid

#246407

Purpose

Expresses K682R_K686R_K69R_K709R POLH with an N-terminal NLS~FLAG tag in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

FLAG

Expression

Mammalian

Mutation

POLH K682R_K686R_K69R_K709R. Coding sequence has …

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_WT POLH_ΔGG NEDD8

Plasmid

#221860

Purpose

Expresses FLAG-tagged WT POLH fused to ΔGG NEDD8 in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

FLAG and ΔGG NEDD8

Expression

Mammalian

Mutation

Coding sequence has been optimised for expression…

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_WT POLH_ΔGG Ubiquitin

Plasmid

#221861

Purpose

Expresses FLAG-tagged WT POLH fused to ΔGG ubiquitin in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

FLAG and ΔGG Ubiquitin

Expression

Mammalian

Mutation

Coding sequence has been optimised for expression…

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_K682A_K709A POLH

Plasmid

#221864

Purpose

Expresses POLH mutated at K682A and K709A with a FLAG tag in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

FLAG

Expression

Mammalian

Mutation

POLH K682A + K709A. Coding sequence has been opti…

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_K682A_K686A_K694A_K709A POLH

Plasmid

#221865

Purpose

Expresses POLH mutated at K682A, K686A, K694A and K709A with a FLAG tag in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

FLAG

Expression

Mammalian

Mutation

POLH K682A, K686A, K694A + K709A. Coding sequence…

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pHR-AL-mCh-Cry2WT-NLS

Plasmid

#221924

Purpose

Expression of CBP autoregulatory loop (AL) fragment fused to optogenetic protein mCh-Cry2WT

Depositor

Daniel Bose

Article

Gelder et al Cell Rep. 2026 Mar 12;45(3):117109. doi:

Insert

CBP AL (aa 1529-1607) (CREBBP Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

insertion of the autoregulatory loop (AL) (aa 152…

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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AOI-WT-Cas9-sg-mouse Gfi1-exon5 (F3)-GFP

Plasmid

#91884

Purpose

Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Gfi1

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

gRNA targeting Gfi1 (Gfi1 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only
AOI-WT-Cas9-sg-mouse Gfi1-exon4(F2)-GFP

Plasmid

#91877

Purpose

Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Gfi1

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

gRNA targeting Gfi1 (Gfi1 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only
AOI-WT-Cas9-sg-mouse Gfi1-exon4(F1)-GFP

Plasmid

#91883

Purpose

Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Gfi1

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

gRNA targeting Gfi1 (Gfi1 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only
pLV[Exp]-EF1A>Tet3G:IRES:Neo

Plasmid

#198756

Purpose

Vector encodes a Tet-On Advanced transactivator under control of a constitutive EF1α promoter and confers resistance to the antibiotic G418

Depositor

Nael Nadif Kasri

Article

Mossink et al Mol Psychiatry. 2022 Jan;27(1):1-18. doi

Insert

Tet3G, IRES, Neo

Use

Lentiviral

Promoter

EF1A

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221-EWSR1 Stop

Plasmid

#84887

Purpose

DONOR vector for Gateway cloning of EWSR1

Depositor

Aaron Gitler

Article

Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91

Insert

EWSR1 (EWSR1 Human)

Use

Gateway donor

Promoter

none

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pAM-DCA-HA-Ast-3-NP-1-IRES-mCherry-WPRE

Plasmid

#159630

Purpose

AAV expression of HA Allatostatin-3, neurophysin, IRES mCherry under the CAG promoter

Depositor

Andrew Allen, Ross Bathgate

Article

Ngo et al Cell Rep. 2020 Sep 15;32(11):108139. doi

Insert

Ast (AstA Fly)

Use

AAV

Tags

HA, IRES, mCherry, and neurophysin-1

Expression

Mammalian

Promoter

DCA (same as CAG promoter)

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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p11d-tRPA-32Asp8

Plasmid

#102617

Purpose

Expression of human RPA with aspartic acid substituted phosphorylation domain

Depositor

Marc Wold

Article

Binz et al J Biol Chem. 2003 Sep 12;278(37):35584-9

Inserts

RPA1 (RPA1 Human)
RPA3 (RPA3 )
RPA2 (RPA2 )

Expression

Bacterial

Mutation

with aspartic acid substituted phosphorylation do…

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-C-6His-mRplp1

Plasmid

#173153

Purpose

mouse Ribosomal Protein Lateral Stalk Subunit P1 (Rplp1) expression plasmid in mammalian cells

Depositor

Brijesh Kumar Singh

Article

Tripathi et al Mol Metab. 2024 Sep;87:101997. doi: 10.1

Insert

ORF clone Ribosomal Protein Lateral Stalk Subunit P1 (Rplp1) (Rplp1 Mouse)

Tags

His

Expression

Mammalian

Mutation

Wild type

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pCSC-CMV-HA-Ast-3-NP-1-IRES-mCherry

Plasmid

#159631

Purpose

expresses HA Allatostatin-3, Neurophysin-1, IRES mCherry under the CSC promoter

Depositor

Andrew Allen, Ross Bathgate

Article

Ngo et al Cell Rep. 2020 Sep 15;32(11):108139. doi

Insert

Ast (AstA Fly)

Use

Lentiviral

Tags

HA, IRES, mCherry, and neurophysin-1

Expression

Mammalian

Promoter

CMV promoter

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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R989-X05-566: His6-MBP-tev-G-Hs.KRAS4b(1-188)

Plasmid

#159546

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188)

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-188) (KRAS Human)

Tags

His6-MBP-tev

Expression

Bacterial

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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