We narrowed to 11,865 results for: Kars

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pLenti-CaMKIIa-hChR2(H134R)-EYFP-WPRE

Plasmid

#20944

Purpose

3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by CaMKIIa promoter for optogenetic activation

Depositor

Karl Deisseroth

Article

Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

Insert

channelrhodopsin-2

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

H134R

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only
pCS2-3HA-Mefv iso1

Plasmid

#92105

Purpose

Expression in eukaryotic cells

Depositor

Feng Shao

Article

Gao et al Proc Natl Acad Sci U S A. 2016 Aug 16;11

Insert

Mefv isoform1 (Mefv Mouse)

Tags

3xHA

Expression

Mammalian

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only
Flag-HA-EIF3S3

Plasmid

#22545

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

EIF3S3(1-348aa) (EIF3H Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 Ub-S297

Plasmid

#169965

Purpose

pDONR221 for Gateway shuttling. Contains the CARD8 C-terminus fused to ubiquitin (co-translationally processed)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Tags

Ub

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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Human DsRed-Monomer CASP9 C287A Mutant

Plasmid

#198469

Purpose

Expresses Human DsRed-Monomer CASP9 C287A Mutant Fusion Protein in Mammalian Cells

Depositor

Hannah Rabinowich

Insert

Human Caspase-9 C287A Mutant (CASP9 Human)

Tags

DsRed-monomer

Expression

Mammalian

Mutation

Changed Cysteine 287 to Alanine to eliminate prot…

Promoter

CMV

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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ptagRFP-MS2coatProtein

Plasmid

#103831

Purpose

Live cell reader for MS2 loop RNA production

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

MS2 coat protein (MCP) (cp )

Tags

tagRFP

Expression

Mammalian

Mutation

MCP: nt 202-240 are missing (13 aa), C242G (A81G)…

Promoter

CMV

Available Since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pPagP

Plasmid

#112125

Purpose

Bacterial expression of outer membrane protein PagP, wild type

Depositor

Karen Fleming

Article

Burgess et al J Biol Chem. 2008 Sep 26;283(39):26748-5

Insert

PagP (pagP E. coli)

Expression

Bacterial

Mutation

N-terminal signal sequence not included and repla…

Promoter

T7

Available Since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-VChR1-EYFP-WPRE

Plasmid

#20954

Purpose

3rd gen lentiviral expression of VChR1 fused to EYFP driven by CaMKIIa promoter for optogenetic activation

Depositor

Karl Deisseroth

Article

Zhang et al Nat Neurosci. 2008 Jun . 11(6):631-3.

Insert

volvox channelrhodopsin-1

Use

Lentiviral

Tags

YFP

Expression

Mammalian

Promoter

CaMKIIa

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pTRE3G-iRFP-B1-timestamp_A

Plasmid

#159924

Purpose

Timestamp Template A inserted into the 3’ UTR of iRFP between bActin Zipcode element and WPRE, with RNA barcode TGC. Also includes a xrRNA element in the 5’ UTR. AKA 147B1.

Depositor

Fei Chen

Article

Rodriques et al Nat Biotechnol. 2020 Oct 19. pii: 10.103

Insert

iRFP-Timestamp A

Expression

Mammalian

Promoter

TRE3G

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-LAP2-mCherry

Plasmid

#159524

Purpose

Expresses mCherry from LAP2 promoter

Depositor

Esteban Engel

Article

Maturana et al Mol Ther Methods Clin Dev. 2020 Apr 14;1

Insert

mCherry

Use

AAV

Expression

Mammalian

Promoter

LAP2

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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NLS-tdPCP-GFP-VPR

Plasmid

#183936

Purpose

PCP tandem dimer binding to PP7 RNA stem loops coupled to VP16 activation domain; labeled with GFP

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

tdPCP-GFP-VPR

Tags

NLS

Expression

Mammalian

Mutation

none

Promoter

CMV (TATA box removed)

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pMD19-Cas9_handle-S.pyogenes_terminator-PBBa_J23117-Cmr

Plasmid

#190794

Purpose

Template vector to amplify pieces for creating multiplex sgRNA-arrays.

Depositor

Paul Hudson

Article

Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

Insert

sgRNA-scaffold only

Use

Synthetic Biology

Expression

Bacterial

Promoter

BBa_J23117

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pmKate2-MS2coatProtein

Plasmid

#103833

Purpose

Live cell reader for MS2 loop RNA production

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

mKate2-MS2 coat protein (cp )

Expression

Mammalian

Mutation

MCP: nt 202-240 are missing (13 aa), C242G (A81G)…

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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PERK 3: PERK.dC.9E10.pCDNA

Plasmid

#21816

Depositor

David Ron

Article

Harding et al Nature. 1999 Jan 21. 397(6716):271-4.

Insert

PERK (C-term deleted) (Eif2ak3 Mouse)

Tags

Myc (9E10)

Expression

Mammalian

Mutation

amino-acids 582-1081 deleted

Available Since

July 27, 2010

Availability

Academic Institutions and Nonprofits only

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pNBA001

Plasmid

#155323

Purpose

Binary vector for expression of PULSE-controlled EFR-GFP

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

LB_(etr)8-(C120)5-PhCMVmin-EFR-GFP-T35S_RB

Use

Synthetic Biology; Eukaryotic expression

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pNBA002

Plasmid

#155324

Purpose

Binary vector for expression of PULSE-controlled GBP-mCherry

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

LB_(etr)8-(C120)5-PhCMVmin-GBP-mCherry-T35S_RB

Use

Synthetic Biology; Eukaryotic expression

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR/zeo-MACROD1

Plasmid

#172572

Purpose

For gateway cloning of N-terminal tagged MACROD1 constructs.

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

MACROD1 (MACROD1 Human)

Use

Gateway cloning

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR/zeo-MACROD2

Plasmid

#172573

Purpose

For gateway cloning of N-terminal tagged MACROD2 constructs.

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

MACROD2 (MACROD2 Human)

Use

Gateway cloning

Available Since

April 7, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-SI-121

Plasmid

#131126

Purpose

A-to-G base editing reporter

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

mutant EGFP

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

CMV promoter

Available Since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pGLACTE-HT51-10

Plasmid

#99255

Purpose

Contains expanded CGG repeats (98 repeats with 2 AGG interruptions) within the 5'UTR of FMR1. To generate capillary electrophoresis size standards.

Depositor

Karen Usdin

Article

Hayward et al J Mol Diagn. 2017 Nov;19(6):828-835. doi

Insert

FMR1 (FMR1 Human)

Tags

eGFP

Available Since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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