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pEY14

Plasmid

#191059

Purpose

acr-5 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

acr-5(orf) (acr-5 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pMy-BirA-T2A-eGFP

Plasmid

#50565

Purpose

Retroviral vector encoding for GFP and the biotinylating enzyme BirA

Depositor

Rafael Casellas

Article

Nakahashi et al Cell Rep. 2013 May 30;3(5):1678-89. doi:

Insert

Ampicilin

Use

Retroviral

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-dInPAkt(R23/25A)

Plasmid

#181835

Purpose

Phospholipid binding-deficient Lyn-dInPAkt negative-control plasmid.

Depositor

Jin Zhang

Article

Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

Insert

Lyn-dInPAkt

Tags

N-terminal targeting sequence from Lyn kinase, dd…

Expression

Mammalian

Mutation

Arginines 23 and 25 in PH domain mutated to alani…

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ChRger2-TS-EYFP

Plasmid

#127237

Purpose

High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.

Depositor

Viviana Gradinaru

Article

Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

ChRger2-TS-EYFP

Use

AAV

Tags

EYFP and SpyTag

Expression

Mammalian

Promoter

hSyn1

Available Since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pBAD-mNectarine

Plasmid

#21717

Purpose

Bacterial expression of pH-sensitive monomeric mNectarine red fluorescent protein

Depositor

Robert Campbell

Article

Johnson et al J Biol Chem. 2009 Jul 31;284(31):20499-5

Insert

mNectarine fluorescent proteins

Tags

His

Expression

Bacterial

Mutation

The fluorescent protein was inserted between XhoI…

Available Since

Aug. 11, 2009

Availability

Academic Institutions and Nonprofits only

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pLD-puro-2A-rtTA-TcVA

Plasmid

#24592

Depositor

Jason Moffat

Article

Mak et al Mol Cell Proteomics. 2010 May;9(5):811-2

Insert

Gateway(TM) cassette

Use

Lentiviral

Tags

VA

Expression

Mammalian

Available Since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only

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pYFJ16-LplA(W37V) (for E. Coli expression)

Plasmid

#34838

Depositor

Alice Ting

Article

Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

Insert

LplA W37V (lplA E. Coli)

Tags

His Tag

Expression

Bacterial

Mutation

Mutated Trp37 to Val

Promoter

T5 promoter

Available Since

May 16, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV-BE4-PpAPOBEC1

Plasmid

#138349

Purpose

Mammalian expression plasmid for BE4-PpABOBEC1

Depositor

Nicole Gaudelli

Article

Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

Insert

BE4-PpAPOBEC1

Tags

BP-NLS

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-HLA-A2_GFP

Plasmid

#85161

Purpose

HLA.A2_GFP fusion with 2 glycines in between

Depositor

Tone Fredsvik Gregers, Sébastien Walchli

Article

Walchli et al Eur J Immunol. 2014 Mar;44(3):774-84. do

Insert

HLA.A2 (HLA-A Human)

Use

Gateway entry vector

Tags

EGFP

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE DN Cullin5

Plasmid

#41916

Depositor

Stephen Elledge

Article

Emanuele et al Cell. 2011 Oct 14;147(2):459-74. doi: 10

Insert

CUL5 (CUL5 Human)

Use

Lentiviral

Available Since

April 3, 2013

Availability

Academic Institutions and Nonprofits only

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pPIK16045_pX330_Flag-SuperFi-Cas9

Plasmid

#184370

Purpose

Mammalian expression of SuperFi-Cas9 nuclease

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

Insert

SuperFi-Cas9

Use

CRISPR

Tags

3X FLAG and NLS

Expression

Mammalian

Mutation

Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q1027D, K…

Promoter

CBh

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-eYFP

Plasmid

#117383

Purpose

An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein eYFP

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Has Service

AAV1

Insert

EYFP

Use

AAV

Expression

Mammalian

Promoter

TRE

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-synp-F-H2B-jRGECO1a

Plasmid

#102995

Purpose

Expresses histone fused jRGECO1a under the synapsin promoter

Depositor

David Cox

Article

Farhi et al J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0

Insert

H2B-jRGECO1a

Use

AAV

Expression

Mammalian

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pET21b-RBD-JA1

Plasmid

#167126

Purpose

Expresses SARS-CoV-2 RBD in bacterial system

Depositor

David Margulies

Article

Ahmad et al bioRxiv. 2021 Jan 27. doi: 10.1101/2021.

Insert

SARS-CoV-2 Receptor Binding domain (S SARS-CoV-2)

Expression

Bacterial

Mutation

residues (333 to 529 aa)

Promoter

T7

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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hDHFR

Plasmid

#184707

Purpose

bacterial expression of human DHFR

Depositor

Peter Wright

Article

Bhabha et al FEBS Lett. 2011 Nov 16;585(22):3528-32.

Insert

DHFR (DHFR Human)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-2Aneo v2

Plasmid

#80033

Purpose

Used to construct AAV-based targeting vectors that carry an IVS-2Aneo cassette for promoter-trapping

Depositor

Hiroyuki Konishi

Article

Karnan et al Nucleic Acids Res. 2015 Dec 10. pii: gkv

Type

Empty backbone

Use

AAV and Cre/Lox; A platform vector to construct t…

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pHAGE DN Cullin3

Plasmid

#41913

Depositor

Stephen Elledge

Article

Emanuele et al Cell. 2011 Oct 14;147(2):459-74. doi: 10

Insert

Cullin 3 (CUL3 Human)

Use

Lentiviral

Available Since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only

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pJAK184.tetR.PT5-3.dCas9

Plasmid

#232482

Purpose

optimized ahTet inducible dCas9 for insertion into C. difficile genome

Depositor

James Collins

Article

Chua et al Microbiol Resour Announc. 2025 Apr 28:e0

Insert

Catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system

Use

CRISPR

Expression

Bacterial

Promoter

TetR-PT5-3

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pJC.15A.sgRNA.TA

Plasmid

#232483

Purpose

sgRNA cloning backbone for expression of sgRNA in C. difficile. Contains TA system to enable maintenence without antibiotic selection.

Depositor

James Collins

Article

Chua et al Microbiol Resour Announc. 2025 Apr 28:e0

Inserts

Toxin-antitoxin System from CD630
medium-copy-number p15A origin of replication
mCherry

Use

CRISPR

Expression

Bacterial

Promoter

P3 constitutive promoter and native promoter

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-AcGFP-progerin

Plasmid

#86947

Purpose

Construct used to synthesize progerin mRNA. Please note the plasmid contains LMNA gene with nucleotide C1824T mutation.

Depositor

Alex Hewitt

Insert

AcGFP-progerin (LMNA Human)

Use

AAV

Tags

AcGFP

Expression

Mammalian

Mutation

progerin sequence is LMNA gene with nucleotide C1…

Promoter

CMV and T7

Available Since

June 7, 2017

Availability

Academic Institutions and Nonprofits only

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