We narrowed to 13,693 results for: crispr cas9

1. pORE303N_CEN1

   Plasmid

   #194439

   Purpose

   CRISPR, Cas9 driven by double 35S, nptII for plant selection, knockout CEN1

   Depositor

   Chung-Jui Tsai

   Article

   Ortega et al New Phytol. 2022 Nov 16. doi: 10.1111/np

   Insert

   gRNA targeting CEN1
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   MtU6

   Available Since

   Jan. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMpGE010-sgRNA-Target1 (MpRTN1)

   Plasmid

   #186295

   Purpose

   Binary vector for CRISPR/Cas9 (target 1: MpRTN1) in plants (for Agrobacterium-mediated genetic transformation)

   Depositor

   Yutaka Kodama

   Article

   Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

   Insert

   sgRNA-Target1
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMCL8_28bp

   Plasmid

   #176185

   Purpose

   plasmid containing scaffold gRNA for Cas9 followed by a 28bp Csy4 recognition sequence, RPR1p-tetO-GFP-scRNA-28bpCsy4-RPR1t

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEPOR1CB0023

   Plasmid

   #117538

   Purpose

   Level1 Golden Gate Cassette: extended stem sgRNA cassette with AtU6-26 terminator for SpCas9, targeting NbPDS gene in N. benthamiana

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   sgRNA (Sp Cas9) - extended stem with U626 terminator
   

   Expression

   Plant

   Available Since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRDA_831

   Plasmid

   #216100

   Purpose

   CRISPRa, EF1a-driven dCas9-VP64 (Cas only)

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   Cas9 [Sp]
   

   Use

   CRISPR and Lentiviral; Assembled vector

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKS7107

   Plasmid

   #89051

   Purpose

   Expresses Nm crRNA, Nm tracrRNA and human codon-optimized NmCas9

   Depositor

   Ervin Welker

   Article

   Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

   Inserts

   Nm crRNA
   Nm tracrRNA
   hNmCas9
   

   Use

   CRISPR

   Tags

   HA tag, NLS, and SV40 NLS

   Promoter

   EF1a and human U6

   Available Since

   April 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PX458_BCL6_iso1_2

   Plasmid

   #104047

   Purpose

   Encodes gRNA for 3' target of human BCL6_iso1 along with Cas9 with 2A GFP

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   BCL6_iso1 gRNA (BCL6 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PX458_BCL6_iso1_1

   Plasmid

   #104046

   Purpose

   Encodes gRNA for 3' target of human BCL6_iso1 along with Cas9 with 2A GFP

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   BCL6_iso1 gRNA (BCL6 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PB_gRNA_S.pyogenes_scaffold_BB

   Plasmid

   #226429

   Purpose

   PiggyBac transposon vector backbone for cloning of sgRNA compatible with S.pyogenes dCas9 enzyme. BbsI Golden Gate site upstream of the scaffold for protospacer insertion. Puromycin selection marker.

   Depositor

   Alon Goren

   Type

   Empty backbone

   Use

   CRISPR; Piggybac transposon

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AcrIIA2

    Plasmid

    #101042

    Purpose

    Anti-CRISPRprotein which inhibit SpyCas9 and LmoCas9 activity.

    Depositor

    Zhiwei Huang

    Article

    Dong et al Nature. 2017 Jun 15;546(7658):436-439. d

    Insert

    AcrIIA2
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AcrIIA4

    Plasmid

    #101043

    Purpose

    Anti-CRISPRprotein which inhibit SpyCas9 and LmoCas9 activity.

    Depositor

    Zhiwei Huang

    Article

    Dong et al Nature. 2017 Jun 15;546(7658):436-439. d

    Insert

    AcrIIA4
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCK668

    Plasmid

    #192637

    Purpose

    Expresses dCas9-NG and MCP-SoxS(R93A/S101A) on p15A-CmR

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

    Inserts

    Sp.pCas9-dCas9-NG
    BBa_J23107-MCP-SoxS(R93A, S101A)
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    SoxS has R93A and S101A mutations and dCas9-NG ha…

    Promoter

    BBa_J23107 and Sp.pCas9

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJF093

    Plasmid

    #113323

    Purpose

    Sp.pCas9-dCas9, pTet-MCP-SoxS_R93A

    Depositor

    Jesse Zalatan

    Article

    Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

    Insert

    dCas9 and MCP-SoxS_R93A
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    R93A

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-ABE8.8m-WT-P2A-EGFP (BKS953)

    Plasmid

    #242651

    Purpose

    CMV promoter expression plasmid for human codon optimized ABE8.8m A-to-G base editor with SpCas9(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

    Insert

    pCMV-ABE8.8m-SpCas9-P2A-EGFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    ABE8.8 mutations in TadA

    Promoter

    CMV and T7

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV-eVRQR-P2A-EGFP (KAC1028)

    Plasmid

    #242663

    Purpose

    CMV promoter expression plasmid for human codon optimized SpCas9-eVRQR(S55R)-BPNLS(SV40)-3xFLAG-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

    Insert

    pCMV-SpCas9-eVRQR(S55R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    eVRQR mutations in SpCas9(S55R/D1135V/G1218R/R133…

    Promoter

    CMV and T7

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-PE2-SpG (HES824)

    Plasmid

    #242689

    Purpose

    Prime editing in mammalian cells with SpG-based PE2 prime editors

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

    Insert

    pCMV-NLS-SpCas9(H840A)-M-MLV-RT(D200N,T306K,W313F,T330P,L603W)-NLS
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    PE2 mutations in MMLV RT, SpG mutations in SpCas9…

    Promoter

    CMV

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSQT1601

    Plasmid

    #53369

    Purpose

    human codon optimized Csy4 and FokI-dCas9 expression plasmid

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    hCsy4-T2A-NLS-hFokI-dCas9-NLS
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFD116

    Plasmid

    #124769

    Purpose

    pFD116 carries dcas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector

    Depositor

    David Bikard

    Article

    Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)

    Inserts

    Ptet
    dcas9
    PpflB sgRNA BsaI
    

    Use

    CRISPR

    Available Since

    Aug. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. T444T

    Plasmid

    #113081

    Purpose

    Enhanced RNAi vector for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites.

    Depositor

    Tibor Vellai

    Article

    Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

    Type

    Empty backbone

    Use

    RNAi

    Expression

    Bacterial

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. reporter-gT1

    Plasmid

    #47320

    Purpose

    TF reporter for gRNA-AAVS1_T1 (RNA-guided dTomato expression)

    Depositor

    George Church

    Article

    Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

    Insert

    minCMV-dTomato_pA
    

    Use

    CRISPR

    Available Since

    Aug. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge