We narrowed to 173,969 results for: HTT

Showing: 10701 - 10720 of 173969 results

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pHLS-EF1a-FKBP12-Gag(HIV)

Plasmid

#138476

Purpose

Expresses FKBP12 fused Gag for packaging FRB-SpCas9 into NanoMEDIC particle.

Depositor

Akitsu Hotta

Article

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

HIV Gag

Tags

FRBP12 and Lynn Myristolation site

Expression

Mammalian

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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enAsABE8e

Plasmid

#138506

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(8e)-dAsCas12a (En)

Expression

Mammalian

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLH02

Plasmid

#117262

Purpose

Genome editing for Lactobacillus brevis ATCC367

Depositor

Sheng Yang

Article

Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

Insert

PorfX-LVISKB_1732-30 , sapK-sappR

Use

Other

Available Since

Dec. 9, 2019

Availability

Academic Institutions and Nonprofits only

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Strep-KIFC1*-mCh

Plasmid

#120169

Purpose

Expresses a chimera of streptavidin, a KIFC1-based retrograde motor and mCherry (fluorescent tag)

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member C1 (KIFC1 Human)

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIFC1: 125-673 aa

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLGB29

Plasmid

#126619

Purpose

Suicide vector for allelic replacement in Bacteroides, inulin selection and aTC-inducible ss-Bfe1 counterselection

Depositor

Laurie Comstock

Article

Garcia-Bayona et al mBio. 2019 Aug 13;10(4). pii: mBio.01762

Type

Empty backbone

Use

Allellic exchange in bacteria

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pBT280

Plasmid

#122610

Purpose

express evoFERNY-BE4max in mammalian cells

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

evoFERNY-BE4max

Expression

Mammalian

Mutation

H102P D104N

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCDH-CMV-mC-G-LC3B-P

Plasmid

#124974

Purpose

evaluate autophagy

Depositor

Kazuhiro Oka

Article

Sutton et al Autophagy. 2018;14(4):637-653. doi: 10.1

Insert

mCherry-GFP-LC3B

Use

Lentiviral

Promoter

CMV

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pSCM001

Plasmid

#124605

Purpose

Expresses mCherry-pHluorin fusion protein in E. coli, a fluorescent reporter for cytoplasmic pH

Depositor

David Summers

Article

Zarkan et al Sci Rep. 2019 Mar 7;9(1):3868. doi: 10.1

Insert

mCherry-pHluorin

Tags

mCherry-(Gly-Gly-Ser)x2-pHluorin translational fu…

Expression

Bacterial

Promoter

pDAB

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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PB-gRNA-Puro

Plasmid

#121121

Purpose

Expression of a gRNA together with a Puromycin resistance

Depositor

Pablo Navarro

Article

Heurtier et al Nat Commun. 2019 Mar 7;10(1):1109. doi:

Insert

gRNA

Use

CRISPR

Expression

Mammalian

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pLV hU6-gRNA(anti-sense) hUbC-VP64-dCas9-VP64-T2A-GFP

Plasmid

#66707

Purpose

expresses a single gRNA and VP64-dCas9-VP64

Depositor

Charles Gersbach

Inserts

hU6-gRNA expression cassette
S.Pyogenes VP64-dCas9-VP64

Use

Lentiviral and Synthetic Biology

Tags

VP64

Mutation

D10A and H840A

Promoter

hU6 and hUbC

Available Since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pET29b-AK1-His

Plasmid

#124135

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Adenylate kinase (AK1 Chicken)

Tags

6x-His

Expression

Bacterial

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-ymScarletI-CaURA3

Plasmid

#118457

Purpose

Yeast tagging vector to create a gene fusion with yeast optimized mScarletI with CaUra3 selection. *An updated version of this plasmid is available: #168055 pFA6a-link-ymScarletI-URA3.

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymScarletI

Expression

Yeast

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pJMP1339

Plasmid

#119271

Purpose

for cloning new sgRNAs

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

dcas9

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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nls-mCherry

Plasmid

#108881

Purpose

Expression of nuclear localized mCherry

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

nls-mCherry

Use

Can be used for mammalian expression or in vitro …

Expression

Mammalian

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pET-21a(+)-AK1-His

Plasmid

#118977

Purpose

Bacterial expression plasmid for Adenylate kinase isoenzyme 1

Depositor

Sebastian Maerkl

Article

Lavickova et al ACS Synth Biol. 2019 Jan 11. doi: 10.102

Insert

adenylate kinase isoenzyme 1 AK1 (AK1 Chicken)

Tags

6x-His

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCF525-EF1a-Hygro-P2A-mCherry-lenti

Plasmid

#115796

Purpose

Expresses mCherry in human cells, contained in a lentiviral vector.

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

mCherry

Use

Lentiviral

Expression

Bacterial and Mammalian

Promoter

EF-1a promoter

Available Since

Oct. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-CMV_TetR-HA-NLS-P2A-BSD-Myc

Plasmid

#113899

Purpose

Mammalian constitutive expression of HA-tagged Tetracyclin repressor protein and Myc-tagged blasticidin deaminase

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Insert

TetR-HA-NLS-P2A-BSD-Myc

Use

Lentiviral

Tags

HA-Myc

Promoter

CMV-MIE

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pV1382

Plasmid

#111436

Purpose

S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR

Depositor

Gerald Fink

Article

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgRNA-BsmBI stuffer

Use

CRISPR

Expression

Yeast

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-tdTomato-WPRE

Plasmid

#104112

Purpose

AAV-mediated expression of tdTomato under the TRE promoter.

Depositor

Takeshi Imai

Article

Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

tdTomato

Use

AAV

Promoter

TRE

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-TIWB-yellow pre-eGRASP(p32)

Plasmid

#111582

Purpose

An AAV vector that expresses yellow pre-eGRASP(p32) under the tetO promoter.

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

yellow pre-eGRASP(p32)

Use

AAV

Promoter

tetO

Available Since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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