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pCAGGS-4493NES

Plasmid

#138373

Purpose

Booster-PKA, a Red-Shifted Genetically Encoded FRET Biosensor for monitoring PKA activity.

Depositor

Michiyuki Matsuda

Article

Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

Insert

Booster-PKA

Expression

Mammalian

Promoter

CAG

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-FluoSTEP-AKAR

Plasmid

#181844

Purpose

Genetically encoded FRET-based sensor for monitoring PKA activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

FluoSTEP-AKAR

Tags

mRuby2 and sfGFP(1-10)

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pJZC34

Plasmid

#62331

Purpose

sgRNA + 2x MS2(wt+f6) with MCP-VP64 effector for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA + 2x MS2(wt+f6) binding module
MCP-VP64

Use

Lentiviral

Tags

VP64

Expression

Mammalian

Mutation

Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

Promoter

CMV and U6

Available Since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST-DHFR F[3]-N (LEU2)

Plasmid

#177798

Purpose

Gateway destination vector to insert genes of interest having a N-terminal DHFR F[3] fusion for DHFR-PCA/BFG-PCA assays.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

Type

Empty backbone

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCR4-TOPO_L31-FLAG-TEV-AviTag_repair_template

Plasmid

#235608

Purpose

Repair template for inserting FLAG-TEV protease cleavage site-AviTag into the C terminus of mouse Rpl31 via CRISPR

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

Rpl31 C-terminus with FLAG-TEV-AviTag and flanking homology arms

Use

CRISPR

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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PtPuc3_diaCas9_sgRNA

Plasmid

#109219

Purpose

Episome based vector with CRISPR/Cas9_sgRNA module for genome editing in Phaeodactylum tricornutum

Depositor

Per Winge

Article

Sharma et al Sci Rep. 2018 Sep 26;8(1):14401. doi: 10

Inserts

LHCF2 promoter
diaCas9
LHCF1 terminator
U6 promoter
sgRNA
U6 3' region

Use

CRISPR and Synthetic Biology

Promoter

LHCF1 terminator, LHCF2 promoter, U6 3' regi…

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV10-hAtg13

Plasmid

#22872

Depositor

Noboru Mizushima

Article

Hosokawa et al Mol Biol Cell. 2009 Apr . 20(7):1981-91.

Insert

autophagy-related genes 13 (ATG13 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Feb. 25, 2010

Availability

Academic Institutions and Nonprofits only
Arf1-GFP

Plasmid

#39554

Depositor

Paul Melancon

Article

Chun et al Mol Biol Cell. 2008 Aug;19(8):3488-500.

Insert

ADP ribosylation factor 1 (ARF1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only

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M3-dCAS9-SunTag (dCas9-SunTag)

Plasmid

#218783

Purpose

Expresses dCAS9-SunTag fusion protein and double marker GFP-T2A-Puro under independent promoters

Depositor

Maja Jagodic

Article

Kakhki et al bioRxiv 2025.03.15.641804; doi: 10.1101/

Inserts

M3-dCAS9-SunTag (dCas9-SunTag)
GFP-T2A-Puro

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF9-2xCSTREP

Plasmid

#136173

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF9

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF9

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-HKU1-Hemagglutinin-esterase

Plasmid

#168902

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-HKU1-Hemagglutinin-esterase (HE )

Use

Gateway-compatible entry vector

Mutation

Codon optimized (H.sapiens)

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HSPA8

Plasmid

#19460

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

HSPA8 (HSPA8 Human)

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pENN239_CRISPRcharm_Kv1

Plasmid

#220838

Purpose

CRISPRcharm Kv1 expression in mammalian cells

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

CRISPRcharm Kv1

Use

CRISPR

Tags

P2A-TagBFP

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pTS118 His14-Avi-28xGS-anti ALFA tag nanobody

Plasmid

#199371

Purpose

Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Insert

anti-ALFA nanobody

Tags

14xHis-Avi

Expression

Bacterial

Mutation

WT

Promoter

T5-LacO

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pFB2G

Plasmid

#195290

Purpose

Bicistronic HSV-TK and mTagBFP resistance for positive/negative selection

Depositor

Scott Lowe

Article

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

HSV-TK 2A mTagBFP

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsEDC4

Plasmid

#66597

Purpose

Expresses lambdaN-HA-tagged EDC4 in mammalian cells

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

EDC4 (EDC4 Human)

Tags

lambdaN-HA

Expression

Mammalian

Promoter

CMV

Available Since

June 24, 2015

Availability

Academic Institutions and Nonprofits only

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pLJC5-3xHA-eGFP-OMP25

Plasmid

#239249

Purpose

3xHA-eGFP-OMP25 lentiviral overexpression vector

Depositor

Jason Cantor

Article

Huggler et al Nat Metab. 2026 Jan 16. doi: 10.1038/s42

Insert

3xHA-eGFP-OMP25 (SYNJ2BP Human)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Promoter

Ubc

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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lenti sgRNA(MS2)_puro backbone

Plasmid

#73795

Purpose

3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 and EF1A

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-pCALM1-SpCas9-miniU6-sgRNAShank3

Plasmid

#213973

Purpose

AAV vector to express SpCas9 driven by pCALM1 promoter for targeting Shank3 locus

Depositor

Zhonghua Lu

Article

Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

Insert

Shank3 sgRNA

Use

AAV and CRISPR

Available Since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-GFP11(x7)-Actin

Plasmid

#181967

Purpose

Human beta actin tagged with 7 copies of GFP11.

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

GFP11(x7)-Actin (ACTB )

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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