We narrowed to 59,250 results for: {{{s}}}

1. pAAV-CMV-Hsp1-Hsp2Cas9-KY-P2A-puro

   Plasmid

   #192133

   Purpose

   Expresses highly specific Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

   Depositor

   Yongming Wang

   Article

   Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

   Insert

   Hsp1-Hsp2Cas9-KY
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Hsp1-Hsp2Cas9 (K390A, Y446A)

   Promoter

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTV-Flnc-5prime-1000-24MS96T-3F_NeoR

   Plasmid

   #174881

   Purpose

   Targeting vector for generating Flnc STREAMING-tag KI cell

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   STREAMING-tag with homology arms for Flnc
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTV-Usp5-5prime-1000-24MS96T-3F_NeoR

   Plasmid

   #174885

   Purpose

   Targeting vector for generating Usp5 STREAMING-tag KI cell

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   STREAMING-tag with homology arms for Usp5
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTV-Wnk1-5prime-1000-24MS96T-3F_NeoR

   Plasmid

   #174886

   Purpose

   Targeting vector for generating Wnk1 STREAMING-tag KI cell

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   STREAMING-tag with homology arms for Wnk1
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Chinchilla–insM

   Plasmid

   #191226

   Purpose

   Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

   Depositor

   Susanne Dobler

   Article

   Mohammadi et al Mol Biol Evol. 2022 Dec 6:msac258. doi:

   Inserts

   ATP1A1
   ATP1B1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   removed methionine at 122 in the protein sequence

   Promoter

   PH and p10

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Sandgrouse–insD

   Plasmid

   #191228

   Purpose

   Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

   Depositor

   Susanne Dobler

   Article

   Mohammadi et al Mol Biol Evol. 2022 Dec 6:msac258. doi:

   Inserts

   ATP1A1
   ATP1B1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   removed aspartic acid at 121 in the protein seque…

   Promoter

   PH and p10

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR4 Ta-SRO1 JN177

   Plasmid

   #192546

   Purpose

   pENTR plasmid Triticum aestivum SRO1, full CDS, cultivar JN177, no stop codon

   Depositor

   Lennart Wirthmueller

   Article

   Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

   Insert

   SIMILAR TO RCD ONE 1 (SRO1), cultivar Jinan 177 (JN177)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR4 Ta-sro1 SR3

   Plasmid

   #192547

   Purpose

   pENTR plasmid Triticum aestivum sro1, full CDS, cultivar SR3, no stop codon

   Depositor

   Lennart Wirthmueller

   Article

   Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

   Insert

   SIMILAR TO RCD ONE 1 (SRO1), cultivar Shanrong No. 3 (SR3)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pACE-K2P4.1-StrepTagII

   Plasmid

   #191474

   Purpose

   Expresses K2P4.1 with C-terminal StrepTagII

   Depositor

   Arthur Laganowsky

   Article

   Zhu et al J Am Chem Soc. 2022 Apr 27;144(16):7048-

   Insert

   K2P4.1 (KCNK4 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   N104Q N108Q

   Promoter

   polH

   Available Since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. mRFP-PA-GFP-RhoAQ63L

    Plasmid

    #187282

    Purpose

    Express pmRFP-PAGFP-RhoA Q63L

    Depositor

    Alpha Yap

    Article

    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

    Insert

    RhoA (RHOA Human)
    

    Use

    Tags

    mRFP-PAGFP

    Expression

    Mammalian

    Mutation

    RhoA Q63L

    Promoter

    CMV, T7

    Available Since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYPQ265N2

    Plasmid

    #174001

    Purpose

    Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-SpRY-UNG for C-G base editing

    Depositor

    Yiping Qi

    Article

    Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

    Insert

    rAPOBEC1-zCas9(D10A)-SpRY-UNG
    

    Use

    CRISPR

    Tags

    3X FLAG, NLS

    Expression

    Plant

    Mutation

    In zCas9(D10A)-SpRY: D10A, A61R, L1111R, D1135L, …

    Promoter

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYPQ265O2

    Plasmid

    #174002

    Purpose

    Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-SpRY-rXRCC1 for C-G base editing

    Depositor

    Yiping Qi

    Article

    Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

    Insert

    rAPOBEC1-zCas9(D10A)-SpRY-rXRCC1
    

    Use

    CRISPR

    Tags

    3X FLAG, NLS

    Expression

    Plant

    Mutation

    In zCas9(D10A)-SpRY: D10A, A61R, L1111R, D1135L, …

    Promoter

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYPQ265L2

    Plasmid

    #174000

    Purpose

    Gateway entry clone (attL1 & attR5) UNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY for C-G base editing

    Depositor

    Yiping Qi

    Article

    Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

    Insert

    UNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY
    

    Use

    CRISPR

    Tags

    3X FLAG, NLS

    Expression

    Plant

    Mutation

    In rAPOBEC1: R33A; in zCas9(D10A)-SpRY: D10A, A61…

    Promoter

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYPQ131-RZ-Cas12j2

    Plasmid

    #173927

    Purpose

    Golden Gate entry vector; empty vector to clone the 1st Cas12j2 crRNA with ribozyme processing

    Depositor

    Yiping Qi

    Article

    Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYPQ132-RZ-Cas12j2

    Plasmid

    #173928

    Purpose

    Golden Gate entry vector; empty vector to clone the 2nd Cas12j2 crRNA with ribozyme processing

    Depositor

    Yiping Qi

    Article

    Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYPQ133-RZ-Cas12j2

    Plasmid

    #173929

    Purpose

    Golden Gate entry vector; empty vector to clone the 3rd Cas12j2 crRNA with ribozyme processing

    Depositor

    Yiping Qi

    Article

    Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYPQ134-RZ-Cas12j2

    Plasmid

    #173930

    Purpose

    Golden Gate entry vector; empty vector to clone the 4th Cas12j2 crRNA with ribozyme processing

    Depositor

    Yiping Qi

    Article

    Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-Duet1-6xHis-TEV-OPTN

    Plasmid

    #190192

    Purpose

    Plasmid for the expression of 6xHis-TEV-OPTN. Internal reference: SMC397

    Depositor

    Sascha Martens

    Article

    Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

    Insert

    optineurin (OPTN Human)
    

    Use

    Tags

    6xHis and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pv6_dPC_2xChromo

    Plasmid

    #179398

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of dPC_2xChromo

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    dPC_2xChromo
    

    Use

    Tags

    AviTag and EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available Since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-FluoSTEP-AKAR (C/Y)

    Plasmid

    #181862

    Purpose

    FluoSTEP-AKAR using split CFP and cpVenus(E172) as the FRET donor and acceptor. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

    Depositor

    Jin Zhang

    Article

    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

    Insert

    FluoSTEP-AKAR (C-Y)
    

    Use

    Tags

    CFP(1-10) and cpVenus(E127)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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