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Ubiquitin WT

Plasmid

#12647

Depositor

Rachel Klevit

Article

Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

Insert

Ubiquitin - wild type (UBC Human)

Expression

Bacterial

Promoter

T7

Available Since

July 31, 2007

Availability

Academic Institutions and Nonprofits only

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opto-RhoGEF-mCherry_pcDNA3.1

Plasmid

#164473

Purpose

Expresses opto-RhoGEF (BcLOV4-GGGSx2-ARHGEF11dh-GGGSx2-mCherry) in a pcDNA3.1 backbone.

Depositor

Brian Chow

Article

Berlew et al Adv Biol. 2021 Sep;5(9):e2100810. doi: 1

Insert

opto-RhoGEF

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pFa6-TID-FRB-NLS-3HA-HIS3MX6

Plasmid

#228023

Purpose

To integrate TID-FRB-NLS fusion at yeast promoter; yeast BioID with HIS3MX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FRB

Tags

HA tag

Expression

Yeast

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pTW199b2

Plasmid

#136928

Purpose

Directed evolution of SpCas9

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

gIII

Use

CRISPR

Expression

Bacterial

Mutation

none

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pFa6-TID-FKBP-3HA-HIS3MX6

Plasmid

#228025

Purpose

To integrate TID-FKBP fusion at yeast promoter; yeast BioID with HIS3MX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FKBP

Tags

HA tag

Expression

Yeast

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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TLCV2 - sgAP2S1 #2

Plasmid

#202757

Purpose

Encodes Doxycycline inducible Cas9 and sgRNA targeting AP2S1

Depositor

Christopher Counter

Article

Zimmerman et al Nat Commun. 2025 Mar 6;16(1):2254. doi:

Insert

gRNA targeting AP2S1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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TLCV2 - sgAP2S1 #1

Plasmid

#202756

Purpose

Encodes Doxycycline inducible Cas9 and sgRNA targeting AP2S1

Depositor

Christopher Counter

Article

Zimmerman et al Nat Commun. 2025 Mar 6;16(1):2254. doi:

Insert

gRNA targeting AP2S1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pDA313

Plasmid

#195214

Purpose

Expression of superfolder GFP (sfGFP) by a hybrid T7 promoter with tetO.

Depositor

Rahul Sarpeshkar

Article

Deng et al Front Bioeng Biotechnol. 2022 Sep 28;10:

Insert

sfGFP with tetO and a hairpin

Expression

Bacterial

Promoter

T7

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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Myc-TEAD4

Plasmid

#24638

Depositor

Kunliang Guan

Article

Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

Insert

TEAD-4 (TEAD4 Human)

Tags

Myc

Expression

Mammalian

Available Since

May 13, 2010

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-10Myc

Plasmid

#181939

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

10xMyc

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pGreen_dualluc_ORF_sensor

Plasmid

#55207

Purpose

Dual-luciferase reporter for microRNA targeting in plants

Depositor

Michael Axtell

Article

Liu et al Plant Cell. 2014 Feb;26(2):741-53. doi:

Inserts

Renilla luciferase
Firefly luciferase

Use

Luciferase; Plant transient expression

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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Flag-hCRM1

Plasmid

#17647

Depositor

Xin Wang

Article

Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

Insert

CRM1 (XPO1 Human)

Tags

3x Flag

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pPOTv7-g418-spGFP(11)

Plasmid

#190257

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Jack Sunter

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

spGFP(11)

Use

For endogenous gene tagging

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-spGFP(1-10)

Plasmid

#190208

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

spGFP(1-10)

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-Tpr

Plasmid

#35024

Depositor

Larry Gerace

Article

Frosst et al J Cell Biol. 2002 Feb 18;156(4):617-30.

Insert

Tpr (TPR Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2012

Availability

Academic Institutions and Nonprofits only

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pNICE-NL2A

Plasmid

#15259

Depositor

Peter Scheiffele

Article

Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

Insert

Neuroligin 2 with insert A (Nlgn2 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Jan. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pG12-SEAP

Plasmid

#170271

Purpose

Secreted alkaline phosphatase (SEAP) expression in mammalian cells. Reporter plasmid.

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

Insert

SEAP

Expression

Mammalian

Promoter

12xUAS with TATA-box minimal promoter

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCR-U6-gRNA-miniCMV-TdTomato

Plasmid

#192656

Purpose

A plasmid encoding miniCMV sgRNA and miniCMV-promoter-TdTomato

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

TdTomato

Expression

Mammalian

Promoter

U6, miniCMV

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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PlayBack-Ef1a-EcoR1

Plasmid

#203311

Purpose

For expression of eukaryotic genes of interest with Ef1a promoter with EcoRI as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-g418-3HA

Plasmid

#247593

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

3xHA

Use

Unspecified

Promoter

read through

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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