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Showing: 10821 - 10840 of 11090 results

  1. pDEST-N2H-C1

    Plasmid
    #125549
    Purpose
    Gateway compatible N2H assay destination vector with C-terminus tagging of nanoluc fragment1
    Depositor
    Marc Vidal
    Article
    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:
    Type
    Empty backbone
    Use
    In vitro expression
    Tags
    NanoLuc fragment1 attached to c-terminus of Gatew…
    Expression
    Mammalian and Yeast
    Promoter
    synthetic promoter containing yeast, mammalian, a…
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    125549-plasmid-map-sequence-id-254837

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  2. pDEST-N2H-C2

    Plasmid
    #125559
    Purpose
    Gateway compatible N2H assay destination vector with C-terminus tagging of nanoluc fragment2
    Depositor
    Marc Vidal
    Article
    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:
    Type
    Empty backbone
    Use
    In vitro expression
    Tags
    NanoLuc fragment2 attached to c-terminus of Gatew…
    Expression
    Mammalian and Yeast
    Promoter
    synthetic promoter containing yeast, mammalian, a…
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    125559-plasmid-map-sequence-id-254701

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  3. pDEST-N2H-N2

    Plasmid
    #125548
    Purpose
    Gateway compatible N2H assay destination vector with N-terminus tagging of nanoluc fragment2
    Depositor
    Marc Vidal
    Article
    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:
    Type
    Empty backbone
    Use
    In vitro expression
    Tags
    NanoLuc fragment2 attached to n-terminus of Gatew…
    Expression
    Mammalian and Yeast
    Promoter
    synthetic promoter containing yeast, mammalian, a…
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    125548-plasmid-map-sequence-id-254836

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  4. pBFC0993

    Plasmid
    #231993
    Purpose
    CRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with negative control (RFP-targeting) spacer
    Depositor
    Brady Cress
    Article
    Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d
    Inserts
    dRfxCas13d
    crRNA with negative control (RFP-targeting) spacer
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23119 and pTet
    Available Since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    231993-plasmid-map-sequence-id-467616

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  9. GABA(A) receptor subunit a2SE

    Plasmid
    #49169
    Purpose
    pHluorin-tagged GABA A receptor subunit (alpha 2) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface
    Depositor
    Tija Jacob, Stephen Moss
    Article
    Tretter et al J Neurosci. 2008 Feb 6;28(6):1356-65. do
    Insert
    GABA(A) receptor subunit alpha-2 (GABRA2 Human)
    Tags
    HA, bovine alpha 1 signal sequence, and pHGFP (pH…
    Expression
    Mammalian
    Mutation
    T343A
    Promoter
    CMV
    Available Since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    49169-plasmid-map-sequence-id-72495

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  10. pEW108

    Plasmid
    #232823
    Purpose
    Expresses yeast compass complex in insect cells
    Depositor
    Cynthia Wolberger
    Article
    Worden et al Elife. 2020 Jan 10;9. pii: 53199. doi: 1
    Insert
    COMPASS (Cps60, Cps50, Cps35, Cps25, Cps15, Flag-Set1, Cps30, Twinstrep-Cps40) (BRE2, SWD1, SET1, SPP1, SWD3, SWD2, SDC1, SHG1 Budding Yeast)
    Tags
    His6-3xFLAG, Twinstrep
    Expression
    Insect
    Mutation
    Set1(762-1080)
    Promoter
    polyhedrin promoter
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232823-plasmid-map-sequence-id-465728

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  11. pINDUCER-21-LCOR-P2A-HOXA9-T2A-HOXA5

    Plasmid
    #97044
    Purpose
    Expresses LCOR, P2A, HOXA9, T2A and HOXA5 in mammalian cells. *Please see notes below on P2A cleaving efficiency.
    Depositor
    George Daley
    Article
    Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature
    Insert
    Ligand Dependent Nuclear Receptor Corepressor, Runt-related transcription factor 1, ETS-related gene
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    Tet on
    Available Since
    Sept. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    97044-plasmid-map-sequence-id-192694

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  12. pThermoCas9i_ctrl

    Plasmid
    #100982
    Purpose
    Expresses the catalytically inactive version of ThermoCas9 (Thermo(d)Cas9) and its sgRNA module
    Depositor
    John van der Oost
    Article
    Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:
    Inserts
    Catalytically inactive variant of the Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
    ThermoCas9 single guide RNA expressing module
    Tags
    None
    Expression
    Bacterial
    Mutation
    changed aspartate 8 to alanine and histidine 582 …
    Promoter
    B. coagulans DSM 1 pta promoter and B. smithii xy…
    Available Since
    Nov. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100982-plasmid-map-sequence-id-392326

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  13. pLKO_HA.NWS.VSVg_NGFR

    Plasmid
    #158243
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HA.NWS.VSVg
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available Since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158243-plasmid-map-sequence-id-329479

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  15. pLKO_HA.FLAG.VSVg_NGFR

    Plasmid
    #158245
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HA.FLAG.VSVg
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available Since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158245-plasmid-map-sequence-id-327706

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  16. pRRLSIN.cPPT.PGK.MD(ND2bHLH)WCS.WPRE

    Plasmid
    #122056
    Purpose
    Lentiviral expression of a MyoD chimeric protein substituted with the NeuroD2 bHLH domain and point mutations to prevent MyoD interaction with PBX/MEIS: W96A, C98A, and S253P
    Depositor
    Stephen Tapscott
    Article
    Fong et al Cell Rep. 2015 Mar 31;10(12):1937-46. do
    Insert
    MD(ND2bHLH)WCS (Myod1, Neurod2 Mouse)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    MyoD chimeric protein substituted with the NeuroD…
    Available Since
    Feb. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    122056-plasmid-map-sequence-id-235399

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  17. pLKO_NWS.FLAG.VSVg_NGFR

    Plasmid
    #158233
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    NWS.FLAG.VSVg
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available Since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158233-plasmid-map-sequence-id-327650

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  18. pdCas9-M-G6

    Plasmid
    #73437
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant G6.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor G6 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available Since
    March 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    73437-plasmid-map-sequence-id-135336

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  19. pLKO_S.NWS.VSVg_NGFR

    Plasmid
    #158348
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    S.NWS.VSVg
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available Since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158348-plasmid-map-sequence-id-330894

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  20. pdCas9-M-3A2

    Plasmid
    #73435
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3A2.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor 3A2 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available Since
    March 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    73435-plasmid-map-sequence-id-135340

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Showing: 10821 - 10840 of 11090 results