We narrowed to 84,030 results for: TRI

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3xFLAG-SENP7

Plasmid

#42886

Depositor

Edward Yeh

Article

Bawa-Khalfe et al Proc Natl Acad Sci U S A. 2012 Oct 23;10

Insert

SENP7 (SENP7 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

T102A, R969C, L996S

Available Since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pKMD106h - intein-Cas9(C574)

Plasmid

#64191

Purpose

Expresses intein-Cas9(C574) in mammalian cells

Depositor

David Liu

Article

Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi

Insert

mammalian codon-optimized S. pyogenes intein-Cas9(C574) - NLS - 3x FLAG

Use

CRISPR

Tags

NLS - 3x FLAG (C terminal on insert)

Expression

Mammalian

Promoter

CMV

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST40-MFRN2

Plasmid

#50546

Purpose

Expression of MFRN2 gene in mammalian cells

Depositor

Vamsi Mootha

Article

Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

Insert

Mrfn2 (SLC25A28 Human)

Tags

V5

Expression

Mammalian

Available Since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only
pZac2.1-GfaABC1D-GAT3-HA-mCherry

Plasmid

#117722

Purpose

Expresses a GABA transporter GAT-3 (Slc6a11)

Depositor

Baljit Khakh

Article

Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

Insert

Slc6a11 (Slc6a11 Mouse)

Use

AAV

Tags

HA and mCherry

Expression

Mammalian

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pAC8-DDIT4

Plasmid

#187116

Purpose

insect cell expression of DDIT4

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

DDIT4 (DDIT4 Human)

Tags

Strep

Expression

Insect

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAF0443 Nm60_attP-mCherry donor

Plasmid

#193471

Purpose

Promoterless mcherry donor for Nm60

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Nm60_attP

Expression

Mammalian

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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PB-CAG::Nucbow

Plasmid

#158996

Purpose

Multicolor nuclear-restricted labeling with a genome-integrating piggyBac transposon vector (randomly expressing H2B-tethered mEYFP, mCherry or mCerulean upon Cre recombination)

Depositor

Jean Livet

Article

Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10

Insert

Nucbow

Tags

All FPs are fused to H2B

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-HsSENP1

Plasmid

#71465

Purpose

Encodes human full length SENP1 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).

Depositor

Jorge Eduardo Azevedo

Article

Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

Insert

SUMO1/sentrin specific peptidase 1 (SENP1 Human)

Tags

Hexahistidine

Expression

Bacterial

Mutation

Gene optimised for E. coli expression (Genscript)

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pAF0466 Ef1a-Nm60_attB acceptor

Plasmid

#193476

Purpose

Acceptor plasmid for 3 plasmid recombination assay

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Nm60_attB

Expression

Mammalian

Promoter

Ef1a

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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EC2_10_dCas9_VPR_HC_sgRNA

Plasmid

#163712

Purpose

Yeast high copy plasmid with dCas9, sgRNA expression cassette and VPR activation domains

Depositor

Yvonne Nygård

Article

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Inserts

dCas9
VPR

Expression

Yeast

Mutation

Point mutations D10A and H840A

Promoter

ScTEF1

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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SP6-VEE-IRES-Puro

Plasmid

#58971

Purpose

noninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

Depositor

Steven Dowdy

Article

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Type

Empty backbone

Use

Venezuelan equine encephalitis (vee) virus rna re…

Expression

Mammalian

Promoter

26S subgenomic promoter

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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GW1-mito-mCherryEA

Plasmid

#207868

Purpose

Mitochondria-targeted mCherry(I158E/Q160A) mutant as a red fluorescent pH sensor

Depositor

Mathew Tantama

Article

Rajendran et al ACS Omega. 2018 Aug 31;3(8):9476-9486. d

Insert

mito-mCherryEA

Tags

4xCox8 mitochondrial targeting

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMT-ADARcd-E488Q-V5

Plasmid

#139686

Purpose

Expresses only the hyperactive form of the ADAR catalytic domain - no RNA binding protein. This is a control plasmid to be used with pMT-Hrp48-ADARcd-E488Q-V5

Depositor

Michael Rosbash

Article

Xu et al RNA. 2018 Feb;24(2):173-182. doi: 10.126

Insert

Adenosine deaminase acting on RNA (Adar Fly)

Tags

V5

Expression

Bacterial and Insect

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pHR CD19-PI3K CAR

Plasmid

#113018

Purpose

for lentivirus production of CD19-PI3K CAR

Depositor

Ron Vale

Article

Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

Insert

CD19 CAR-P intracellular PI3k recruiting domain from CD19 (CD19 Human, Synthetic)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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MMLV-RT(dRH) for Split-PE (pEH50)

Plasmid

#190106

Purpose

Expresses MMLV-RT (from PE2) with truncated RNAse H domain (dRH) for Split-PE use

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

Insert

MMLV-RT(delta RNAseH)

Tags

bpNLS

Expression

Mammalian

Mutation

mutations from RT in PE2 and truncation of RNAse …

Promoter

CMV

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAF0185 Cp36_attP-mCherry donor

Plasmid

#193468

Purpose

Promoterless mcherry donor for Cp36

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Cp36_attP

Expression

Mammalian

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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GW1-rogRFP2

Plasmid

#207876

Purpose

Mammalian expression of the red fluorescent rogRFP2 redox sensor

Depositor

Mathew Tantama

Article

Radhakrishnan et al ACS Chem Neurosci. 2020 Sep 2;11(17):266

Insert

rogRFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pKH161_FLAG-APEX2-YIPF3 WT

Plasmid

#214980

Purpose

Flag-APEX2 tagged YIPF3

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

FLAG-APEX2-YIPF3 (YIPF3 Human)

Use

Lentiviral

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-HsSENP2

Plasmid

#71466

Purpose

Encodes human full length SENP2 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).

Depositor

Jorge Eduardo Azevedo

Article

Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

Insert

SUMO1/sentrin specific peptidase 2 (SENP2 Human)

Tags

Hexahistidine

Expression

Bacterial

Mutation

Gene optimised for E. coli expression (Genscript)

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
phMYT1L-N106

Plasmid

#60861

Purpose

Expresses MYT1L under the EF1a promoter

Depositor

Andrew Yoo

Article

Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

Insert

MYT1L (MYT1L Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

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