We narrowed to 23,148 results for: REP

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pCoofy40

Plasmid

#44006

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

His10-Stop-OneStrep-Stop-S-Stop-CBP-Stop-HPC4-Sto…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pSMP-G9A_3

Plasmid

#36333

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

G9A (EHMT2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pBABE flag M33

Plasmid

#1951

Depositor

Robert Kingston

Article

Levine et al Mol Cell Biol 2002 Sep;22(17):6070-8.

Insert

M33 (Cbx2 Mouse)

Use

Retroviral

Tags

flag

Expression

Mammalian

Available Since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
AAV-KrasG12V/sgKras/Cre

Plasmid

#99854

Purpose

Expresses Cre-recombinase, a barcoded HDR template to introduce a G12V mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

Depositor

Monte Winslow

Article

Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

Insert

Kras (Kras Mouse)

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-UBC-S3-ExRaiAKAR

Plasmid

#160724

Purpose

Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter ExRai-AKAR (PKA indicator), V5 tag, and S3 protein scaffold.

Depositor

Edward Boyden

Article

Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

Insert

S3-ExRaiAKAR

Use

AAV

Expression

Mammalian

Mutation

N/A

Promoter

Human ubiquitin C (UBC) promoter

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pMuLE_ENTR_PTCH1-mTurquoise2_R4-R3

Plasmid

#113864

Purpose

Single pathway reporter; hedgehog promoter-driven expression of mTurquoise2; MultiSite Gateway entry vector.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Inserts

PTCH1 (hedgehog dependent promoter)
mTurquoise2

Use

Gateway Cloning

Expression

Mammalian

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pLVH-EF1a-GFP-P2A-KLF4

Plasmid

#130694

Purpose

Lentiviral plasmids encoding human KLF4 with co-expressoin of GFP mediated by P2A.

Depositor

Kejin Hu

Article

Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

Insert

human KLF4 (KLF4 Human)

Use

Lentiviral

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCAP03-ermE*p

Plasmid

#120234

Purpose

Carries promoter cassette containing ermE*p promoter and ampicillin resistance gene that can be amplified with homology sequence for refactoring.

Depositor

Bradley Moore

Article

Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

Insert

promoter cassette containing ermE*p promoter and ampicillin resistance gene

Use

Synthetic Biology

Mutation

bla (amp resistance gene)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_HAND2_P2A_Hygro_Barcode

Plasmid

#120447

Purpose

Barcoded lentiviral vector to express HAND2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

HAND2 (HAND2 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pSMP-DNMT1_2

Plasmid

#36383

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

DNMT1 (DNMT1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

June 22, 2012

Availability

Academic Institutions and Nonprofits only
pSUMO His6 SUMO MYC3

Plasmid

#177848

Purpose

Bacterial Expression of MYC3

Depositor

Karsten Melcher

Article

Zhang et al Nature. 2015 Sep 10;525(7568):269-73. do

Insert

MYC3 (MYC3 Mustard Weed)

Tags

6xHis-SUMO

Expression

Bacterial

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pJet1.2 cat cassette

Plasmid

#117119

Purpose

Chloramphenicol resistance cassette

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Insert

Chloramphenicol resistance cassette for Bacillus subtilis selection

Use

Golden gate donor vector for gene targeting in ba…

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pJet1.2 specR cassette

Plasmid

#117120

Purpose

Spectinomycin resistance cassette

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Insert

Spectinomycin resistance cassette for Bacillus subtilis selection

Use

Golden gate donor vector for gene targeting in ba…

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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Non-specific sgRNA

Plasmid

#109432

Purpose

MLM3636 backbone containing a gRNA that does not bind to any sequence in the human genome.

Depositor

Reuben Harris

Article

St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

Insert

Non-specific gRNA

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

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pCI-OR2J2

Plasmid

#22321

Depositor

Hiroaki Matsunami

Article

Saito et al Sci Signal. 2009 . 2(60):ra9.

Insert

OR2J2 (OR2J2 Human)

Tags

Rho

Expression

Mammalian

Available Since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

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pAd5-B1-deltaE1-MCS

Plasmid

#122558

Purpose

Modified block 1, with deletion in E1

Depositor

Fred Bunz

Article

Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

Insert

Adenovirus 5 genomic region 1-3759

Use

Synthetic Biology

Mutation

Adenovirus sequences 481-3530 replaced with multi…

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pTrc99S-ssDsbA-CRM197-4xDQNAT

Plasmid

#128403

Purpose

Periplasmic expression of CRM197 with C terminal 4xDQNAT glycosylation sites in E. coli

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Diphtheria toxin

Tags

4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

Expression

Bacterial

Mutation

Inactivating G52E mutation

Promoter

trc

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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CD3-2A_pMI-LO

Plasmid

#153418

Purpose

Retroviral (MSCV) expression of all four WT CD3 subunits, co-expressed with LSSmOrange

Depositor

Maki Nakayama

Article

Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

Insert

murine CD3 delta-gamma-epsilon-zeta subunits (Cd3d Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF6_P2A_Hygro_Barcode

Plasmid

#120492

Purpose

Barcoded lentiviral vector to express KLF6 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF6 (KLF6 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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RAB11A-mCherry HDRT Source (pTR 144)

Plasmid

#112013

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with mCherry

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

RAB11A-mCherry HDRT (RAB11A Human)

Use

CRISPR and Synthetic Biology

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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