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pcDNA3.1+ Flag-RAP2A

Plasmid

#128099

Purpose

Expresses RAP2A in mammalian cells in a G418-selectable plasmid

Depositor

Chi Van Dang

Article

Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

Insert

RAP2A (RAP2A Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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HA-Rab8a-CA (Q67L)

Plasmid

#101050

Purpose

Expresses HA-tagged human Rab8-CA (Q67L) in mammalian cells

Depositor

Ken-Ichi Takemaru

Article

Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

Insert

Rab8a (RAB8A Human)

Tags

HA

Expression

Mammalian

Mutation

Q67L

Promoter

CMV

Available Since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV_T7-PE2-Nuclease

Plasmid

#171997

Purpose

Sequence provided for PE-Nuclease mRNA transcription, suitable for microinjection

Depositor

Paul Thomas

Article

Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

Insert

CMV_T7-Cas9-RT

Expression

Mammalian

Mutation

GC to CA point mutation in SpCas9 to restore nucl…

Promoter

CMV for Cas9, U6 for gRNAs

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pdest WDR4 Puro

Plasmid

#223062

Purpose

WDR4 gene expression in mammalian cells

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

WDR4 (WDR4 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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LARG mCherry sspB in pcDNA3.1

Plasmid

#90459

Purpose

RhoA mediated cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

Inserts

LARG DH-PH (aa 766–1138) (ARHGEF12 Human)
mCherry
sspB

Expression

Mammalian

Mutation

R73Q

Promoter

CMV

Available Since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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GG-EBNA-MIR302-7guides-PGK-Puro

Plasmid

#201960

Purpose

EBNA episome plasmid for U6 promoter-driven expression of 7 gRNAs targeting miRNA302/367. Includes PGK-puro selection cassette

Depositor

Timo Otonkoski

Article

Sokka et al Stem Cell Reports. 2022 Feb 8;17(2):413-

Insert

MIR302-7g-PGK-Puro

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 6, 2023

Availability

Academic Institutions and Nonprofits only

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AAV_CMV_CCR6-Nanoluc-V5

Plasmid

#199672

Purpose

expresses CCR6-NanoLuc-V5 in mammalian cells, AAV vector

Depositor

Alice Ting

Article

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

CCR6 (CCR6 Human)

Use

AAV

Tags

NanoLuc and V5

Expression

Mammalian

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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PL-5LTR-GW-A

Plasmid

#138480

Purpose

For expression of ribozyme-flanked sgRNA after the Gateway (GW) LR cloning.

Depositor

Akitsu Hotta

Article

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

Gateway attR1/R2 cassette with chloramphenicol resistance and ccdB genes

Expression

Mammalian

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-PQBP1

Plasmid

#25804

Depositor

Marius Sudol

Article

Tapia et al J Biol Chem. 2010 Jun 18;285(25):19391-4

Insert

PQBP1 (PQBP1 Human)

Tags

6xHA

Expression

Mammalian

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only
CL20_mEGFP_ZCCHC7_PAX5

Plasmid

#205958

Purpose

Express mEGFP-tagged fusion protein, ZCCHC7_PAX5 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

ZCCHC7_PAX5 (ZCCHC7, PAX5 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO-shC

Plasmid

#21963

Depositor

Lynda Chin

Article

Ji et al Nature. 2007 Aug 16. 448(7155):807-10.

Insert

si NEDD9 (NEDD9 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 3, 2009

Availability

Academic Institutions and Nonprofits only
HA-YAP-wt

Plasmid

#229412

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

YAP-wt (YAP1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

EFS

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-IRES-Puro PFKM

Plasmid

#201638

Purpose

Over-expression of untagged human PFKM

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

PFKM (PFKM Human)

Use

Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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PXPR007 sgCIC-2

Plasmid

#74959

Purpose

Cas9 + sgCIC-2 with blasticidin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

CIC (CIC Human)

Use

CRISPR

Expression

Mammalian

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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HMGN1_pLENTI-CAG-IRES-GFP

Plasmid

#176995

Purpose

Mammalian lentiviral expression vector encoding HMGN1

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

HMGN1 (HMGN1 Human)

Use

Lentiviral

Promoter

CAG

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-REX-GECO1

Plasmid

#61248

Purpose

Expresses REX-GECO1 in neurons

Depositor

Robert Campbell

Article

Wu et al Nat Commun. 2014 Oct 31;5:5262. doi: 10.

Insert

REX-GECO1

Use

AAV

Expression

Mammalian

Mutation

Substitutions relative to R-GECO1: P60R, V61W, R6…

Promoter

hSyn

Available Since

March 27, 2015

Availability

Academic Institutions and Nonprofits only

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pET-28a RUVBL2 Full Length

Plasmid

#159142

Purpose

Expresssion and protein purification of full length RUVBL2

Depositor

Eric Erquan Zhang

Article

Ju et al Sci Transl Med. 2020 May 6;12(542). pii:

Insert

RUVBL2 (RUVBL2 Human)

Expression

Bacterial

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX FynA-SH2

Plasmid

#46429

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

FynA (FYN Human)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 142-251

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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HA-Rab8a-DN (T22N)

Plasmid

#101049

Purpose

Expresses HA-tagged human Rab8-DN (T22N) in mammalian cells

Depositor

Ken-Ichi Takemaru

Article

Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

Insert

Rab8a (RAB8A Human)

Tags

HA

Expression

Mammalian

Mutation

T22N

Promoter

CMV

Available Since

Oct. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pT2K-LRL-IRES-Luc2

Plasmid

#109230

Purpose

For Tol2 transposon-mediated stable expression of LRL and IRES-luciferase

Depositor

Martine Roussel

Article

Kawauchi et al Oncogene. 2017 Sep 14;36(37):5231-5242.

Insert

LRL and Luc2

Expression

Mammalian

Promoter

CAGGS

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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