We narrowed to 37,610 results for: ELL
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Plasmid#69569PurposeTCR beta chain genomic transgenic construct for creating OT-1 miceDepositorPublicationAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only
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pLKO.1 shPIK3CD.v1 puro
Plasmid#58706PurposeLentiviral shRNA vector for knockdown of human PIK3CDDepositorAvailable sinceSept. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Tau(T217E)
Plasmid#187024PurposeExpresses EGFP tagged human 2N4R tau (with T217E mutation) in mammalian cells with CMV promoterDepositorInsertMAPT (MAPT Human)
TagsEGFPExpressionMammalianMutationChanged Threonine 217 to Glutamic acidPromoterCMVAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
MF85_PB-14xUAS-12x42bs-10x(ErbB2bs_ErbB2bs)-miniCMV-NLS-FKBP12F36V-ErbB2ZFR2AR39A-VP16-NLS-DHFR-IRES-mTurquoise2-PEST-BGHpA
Plasmid#176629PurposeTF C self-activation cassette in MultiFate-3DepositorInsert14xUAS-12x42bs-10x(ErbB2bs_ErbB2bs)-miniCMV-NLS-FKBP12F36V-ErbB2ZFR2AR39A-VP16-NLS-DHFR-IRES-mTurquoise2-PEST-BGHpA
UseSynthetic BiologyAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti NLuc398-hLGALS8 IRES hCALCOCO2-CLuc394
Plasmid#128387PurposeExpresses a split firefly luciferase reporter based on the full length, human Gal8 and CALCOCO2, which interact following endosomal disruptionDepositorUseLentiviral, Luciferase, and Synthetic BiologyTagsCLuc394 and NLuc398ExpressionMammalianPromoterEFS and IRESAvailable sinceJan. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
KCC2-pHext
Plasmid#104076PurposeTo visualize the expression and localization of potassium-chloride cotransporter type 2 (KCC2)DepositorInsertKCC2 (b isoform) (Slc12a5 Rat)
TagspHluorine tag inserted in the second putative tra…ExpressionMammalianMutationEcoR1 site in rat KCC2 was removed by silent muta…PromoterUbCAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-tagBFP-hTRF2
Plasmid#103803PurposeBLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorExpressionMammalianMutationhTRF2: deletion of amino acids 1-42 and 476 compa…PromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSF4 TetCMV 5'TOP intron Renilla-STOP 24xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyA
Plasmid#104140PurposeExpresses TREAT-5'TOP reporter in mammalian cellsDepositorInsertRenilla luciferase
Tags24xMS2 stem loops in 3'UTR, 24xPP7 stem loop…ExpressionMammalianAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 myc-mβTrCP WD1-7 (241-569)
Plasmid#62977PurposeExpresses myc-tagged mouse βTrCP residues 241-569DepositorAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-nAChr-Alpha6-GFP
Plasmid#50486Purposethis report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRFDepositorInsertnAChr-alpha6 (Chrna6 Mouse)
TagsGFP fusion in M3-M4 loop (after residue A405)ExpressionMammalianMutationGly-Ala-Gly flexible linker flanking the GFP open…PromoterCMVAvailable sinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pWJB30
Plasmid#126505PurposeSalmonella typhimurium diguanylate cyclase AdrA cloned as AdrA-8xHis in pBAD30DepositorInsertadrA-8xHis
Tags8xHisExpressionBacterialPromoterPBAD30Available sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTE4999
Plasmid#107555PurposeExpresses human codon optimized inactive FnCpf1(dead1) in mammalian cells.DepositorInserthFnCpf1(dead1)
UseCRISPRTags3xHA and NLSExpressionMammalianMutationD917APromoterCMVAvailable sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmKikGR Actin
Plasmid#105315PurposePhotoconvertable cytoskeletal protein actin to study kinetics or dynamicsDepositorAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
NFATc2-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)
Plasmid#75232PurposeCRISPR/Cas9 plasmid against human NFATc2DepositorInsertsgRNA against NFATc2 (NFATC2 Human)
UseCRISPRAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDest490-OPN-10 kDa
Plasmid#17595DepositorAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
LB360 (Bcl-2 from -1287 to -1337)
Plasmid#15242DepositorInsertB-cell lymphoma/leukemia-2 (BCL2 Human)
UseLuciferaseTagsLuciferaseExpressionBacterialMutationBcl-2 fragment from -1287 to -1337 with P1Available sinceAug. 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
Human Transporter Knockout library
Pooled library#213695PurposeNext generation transporter-focused CRISPR/Cas9 library targeting transmembrane transporters to test the impact of transporters on biological processes.DepositorSpeciesHomo sapiensUseCRISPR and LentiviralAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
S. rosetta Col- cDNA amplified library
Pooled library#62843Purposeamplified library of Salpingoeca rosettaDepositorExpressionBacterialSpeciesSalpingoeca rosettaAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV_AR-RE_MLPmin_BC1011-luc2
Plasmid#227106PurposeAndrogen receptor response element for luciferase and barcode assays; barcode BC1011DepositorInsert8x clustered AR response element linked to MLP minimal promoter driving barcode 1011 and a luciferase reporter gene
UseAAVExpressionMammalianAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCIneoLuc-PP2A
Plasmid#128349PurposeVector for LUMIER assayDepositorAvailable sinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only