We narrowed to 28,145 results for: STI

Showing: 10981 - 11000 of 28145 results

Results per page:

Sort results by:

SpCas9-NRCH-YE1-BE4max

Plasmid

#198549

Purpose

Expresses human codon-optimized SpCas9-NRCH-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-NRCH-YE1-BE4max-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-NRCH-YE1-BE4max

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Promoter

EFS

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR P2R-P3-EGFP

Plasmid

#48349

Purpose

Contributes the coding sequence for EGFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

Depositor

Anna Planas, Tomas Santalucia

Article

Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

Insert

EGFP

Use

Gateway entry vector

Mutation

Aminoacid 40 is valine in our sequence, while it …

Promoter

none

Available Since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
BB3cH_pGAP_23*_pPFK300_Cas9

Plasmid

#104912

Purpose

hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg

Depositor

Brigitte Gasser

Article

Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

Inserts

HH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)

Use

CRISPR

Expression

Yeast

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV_dCas9-ZIM3-KRAB_sgTAOK2i_#3

Plasmid

#172985

Purpose

CRISPRi for TAOK2

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

sgTAOK2i (TAOK2 Human)

Use

CRISPR and Lentiviral

Tags

3xFLAG

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-TetON-mEGFP-MYOD1_WT

Plasmid

#215617

Purpose

For integration of MYOD1 WT T2A mEGFP

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

MYOD1 (MYOD1 Human)

Tags

T2A mEGFP

Expression

Mammalian

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-TetON-mEGFP-MYOD1_AroPERFECT-C

Plasmid

#215621

Purpose

For integration of MYOD1 AroPERFECT C T2A mEGFP

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

MYOD1 AroPERFECT C (MYOD1 Human)

Tags

T2A mEGFP

Expression

Mammalian

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
SpCas9-NG-ABE8.17-m+V106W

Plasmid

#198534

Purpose

Expresses human codon-optimized SpCas9-NG-ABE8.17-m+V106W and blasticidin resistance: EFS promoter-SpCas9-NG-ABE8.17-m+V106W-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-NG-ABE8.17-m+V106W

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Promoter

EFS

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5 bovine GRK5

Plasmid

#14690

Depositor

Robert Lefkowitz

Article

Pitcher et al J Biol Chem. 1996 Oct 4. 271(40):24907-1

Insert

GRK5 (GRK5 Bovine)

Expression

Mammalian

Available Since

June 19, 2007

Availability

Academic Institutions and Nonprofits only
pEMS2159

Plasmid

#70120

Purpose

Promoterless AAV plasmid with MCS to insert promoter for expression of iCre. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-MCS-iCre-WPRE

Use

AAV

Expression

Mammalian

Promoter

Promoterless

Available Since

May 12, 2016

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
MMW1578: CAG-human dLbCpf1(D832A)-NLS-3xHA

Plasmid

#104563

Purpose

Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1)

Depositor

Keith Joung

Article

Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

Insert

dLbCpf1 (D832A)

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Mutation

D832A

Promoter

CAG

Available Since

Jan. 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-FRT/TO-mNeonGreen-NUT

Plasmid

#204693

Purpose

This plasmid allows for inducible expression of full-length NUT tagged with mNeonGreen, in mammalian cells.

Depositor

Michael Rosen

Article

Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

Insert

NUT (NUTM1 Human)

Tags

mNeonGreen

Expression

Mammalian

Mutation

n/a

Promoter

CMV

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSynapsin_NLS_GFP_P2A_His6_NR1.

Plasmid

#170457

Purpose

Expresses histidine tagged NR1 in neuronal cells

Depositor

Shigeki Watanabe

Article

Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

Insert

NR1 (Grin1 Rat)

Use

Lentiviral

Tags

Hexa-histidin

Expression

Mammalian

Promoter

Synapsin

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
hPlekhm1-dsRedM

Plasmid

#73592

Purpose

Express human Plekhm1 in mammalian cells

Depositor

Paul Odgren

Article

Van Wesenbeeck et al J Clin Invest. 2007 Apr;117(4):919-30.

Insert

Plekhm1 (PLEKHM1 Human)

Tags

dsRed

Expression

Mammalian

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only
pCAG_GST-TEV-WIPI3

Plasmid

#223800

Purpose

Expression of recombinant protein for purification

Depositor

James Hurley, Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

WIPI3 (WDR45B Human)

Tags

GST-TEV

Expression

Mammalian

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pX458_Ruby

Plasmid

#110164

Purpose

Cas9 from S. pyogenes with Ruby2, and cloning backbone for sgRNA

Depositor

Philip Hublitz

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Insert

hSpCas9

Use

CRISPR

Tags

3xFLAG and Ruby2

Expression

Mammalian

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4.26-Ccnd2PromoterRegion1(1.2kb)

Plasmid

#228058

Purpose

1.2 kb from mouse Ccnd2 promoter in front of firefly luciferase reporter

Depositor

Kenneth Chen

Article

Fraire et al bioRxiv [Preprint]. 2024 Apr 30:2024.04.

Insert

Ccnd2 promoter (Ccnd2 Mouse)

Use

Luciferase

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Sec61 beta pAcGFP-C1 (802)

Plasmid

#62008

Purpose

mammalian expression of GFP-Sec61 beta

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

Sec61 beta (SEC61B Human)

Tags

AcGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pMCS.DT-A

Plasmid

#22730

Depositor

Mark Magnuson

Article

Chen et al Dis Model Mech. 2011 Jul;4(4):537-47. do

Insert

DT-A

Use

Bac recombineering

Mutation

This is a gap-repair vector designed for retrievi…

Available Since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
spCas9-NLS-MAV

Plasmid

#124212

Purpose

SpCas9-NLS 17 amino acid linker to monoavidin (SpCas9-NLS-MAV)

Depositor

Uri David Akavia

Article

Roche et al CRISPR J. 2018 Dec;1:414-430. doi: 10.10

Insert

cas9

Use

CRISPR

Tags

His6, MBP, TEV Cleavage site, and two copies of S…

Expression

Bacterial

Promoter

T7

Available Since

June 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBa.GFP-KIF13B tail 442-1843

Plasmid

#134623

Purpose

Mammalian expression of mouse KIF13B 442-1843

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF13B (Kif13b Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

WT with deletion of the 441 N-terminal residues.

Promoter

Chicken Beta actin

Available Since

Oct. 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge