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pFPL-Gh

Plasmid

#61648

Purpose

Gateway vector for generating green fluorescent protein fusions in fungi

Depositor

Mark Farman

Article

Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

Insert

enhanced green fluorescent protein

Expression

Plant

Available Since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pTK_TFAP2b_enh_226_Citrine

Plasmid

#130618

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

TFAP2b_enh_226

Use

Enhancer reporter

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pJAT39

Plasmid

#204289

Purpose

For marking CRISPR HDR integrant with EGFP expressed in flight muscles.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

MHC-EGFP+H3:H26

Use

CRISPR

Promoter

MHC

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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miR155 in pcDNA3.1

Plasmid

#78126

Purpose

Mammalian expression of miR155

Depositor

Heidi Schwarzenbach

Article

Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

Insert

miR155 (MIR155 Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pCMVhTRIM65_3Flag

Plasmid

#53966

Purpose

Human TRIM65 mammalian expression vector with 3xFLAG epitope at c-terminus.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

TRIM65 (TRIM65 Human)

Tags

3x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2014

Availability

Academic Institutions and Nonprofits only

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pMaCTag-05

Plasmid

#119984

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with eGFP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

eGFP

Use

Pcr template

Tags

eGFP

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCMX/cyclinB1 (17)

Plasmid

#26060

Depositor

Jonathon Pines

Article

Hagting et al Curr Biol. 1999 Jul 1. 9(13):680-9.

Insert

Cyclin B1 (CCNB1 Human)

Tags

Myc

Expression

Mammalian

Available Since

Aug. 27, 2010

Availability

Academic Institutions and Nonprofits only
GFP-Rnd3

Plasmid

#23229

Depositor

Channing Der

Article

Roberts et al J Biol Chem. 2008 Sep 12. 283(37):25150-

Insert

Rnd3 (RND3 Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only

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Frb-N-TEV

Plasmid

#100095

Purpose

SNIPer plasmid, N terminal TEV fused to Frb

Depositor

Jim Wells

Article

Morgan et al Proc Natl Acad Sci U S A. 2015 Jul 7;112

Insert

N-TEV

Use

Retroviral

Tags

Frb and Myc

Expression

Mammalian

Mutation

N terminal domain

Promoter

CMV

Available Since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pFH2.214_mCherry-PP7x12

Plasmid

#205556

Purpose

Express mCherry-PP7x12 (export tag consisting of 12 tandem repeats of PP7 stem-loop aptamer)

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

mCherry

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pET11a-SUMO2

Plasmid

#53142

Purpose

E.coli expression of untagged mature human SUMO2 (amino acids 1-92)

Depositor

Frauke Melchior

Article

Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

Insert

SUMO2 (SUMO3 Human)

Expression

Bacterial

Mutation

mature form (amino acids 1-92)

Promoter

T7

Available Since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE39

Plasmid

#90379

Purpose

PPARg - PPARg homodimer gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

PPARg

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pME-minP:luc2

Plasmid

#138354

Purpose

Gateway compatible middle entry clone contains Firefly Luciferase2 insert driven by minimal promoter

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

Luciferase

Expression

Mammalian

Promoter

minimal promoter

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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TLCV2 - sgPTEN

Plasmid

#202759

Purpose

Encodes Doxycycline inducible Cas9 and sgRNA targeting PTEN

Depositor

Christopher Counter

Article

Zimmerman et al Nat Commun. 2025 Mar 6;16(1):2254. doi:

Insert

gRNA targeting PTEN

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE42

Plasmid

#90380

Purpose

GATA3 - Gata3 gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

GATA3

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pHV738

Plasmid

#117476

Purpose

Expression of NMT1

Depositor

Richard Kahn

Article

Van Valkenburgh et al Methods Enzymol. 2002;344:186-93.

Insert

NMT1 (NMT1 Human)

Expression

Bacterial

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pBzCas13b-gRNA-NT

Plasmid

#164861

Purpose

Constitutive expression of single-spacer CRISPR array with non-targeting spacer for BzCas13b in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

BzCas13b-gRNA-NT

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pBzCas13b-gRNA-1

Plasmid

#164858

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for BzCas13b in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

BzCas13b-gRNA-1

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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Epsin1-ECFP

Plasmid

#21066

Depositor

Xiaowei Zhuang

Article

Chen et al Proc Natl Acad Sci U S A. 2008 Aug 19. 1

Insert

Epsin 1 (Epn1 Rat)

Tags

ECFP

Expression

Mammalian

Available Since

May 22, 2009

Availability

Academic Institutions and Nonprofits only
pCIP

Plasmid

#79009

Purpose

For expression in mouse ESCs or other eukaryotic cells. Contains pCAGGS-IRES-Puro-bpA, with a NheI-MfeI cloning site in between pCAGGS and IRES.

Depositor

Reuben Shaw

Article

Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

Type

Empty backbone

Expression

Mammalian

Promoter

pCAGGs

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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