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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 28,117 results for: GFP

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Showing: 11041 - 11060 of 28117 results

  2. pYPQ-dSpRY-Act3.0

    Plasmid
    #158416
    Purpose
    CRISPR-Act3.0 system containing dSpRYCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.
    Depositor
    Yiping Qi
    Publication
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Insert
    dSpRY-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available since
    July 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    158416-plasmid-map-sequence-id-337116

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  3. pCS2+ GFP-2xESP3I-IRES-mCherry

    Plasmid
    #230996
    Purpose
    Protein stability reporter construct for transient expression in mammalian cells. Stability of N-terminal GFP-fusion protein can be assessed by flow cytometry by normalizing to mCherry expression.
    Depositor
    Michael Rape
    Publication
    Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:
    Type
    Empty backbone
    Tags
    AcGFP1
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    230996-plasmid-map-sequence-id-457945

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  6. pCDH-EF1-IRES plum

    Plasmid
    #128072
    Purpose
    gfp was exchanged to plum
    Depositor
    Oskar Laur
    Insert
    plum
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    no
    Available since
    Aug. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    128072-plasmid-map-sequence-id-255193

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  9. pcDNA3 H2B-mIFP T2A Mpro (wt)

    Plasmid
    #163079
    Purpose
    Expresses wild type Mpro (active) and a near-infrared fluorescent protein mIFP fused to H2B
    Depositor
    Xiaokun Shu
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Wild type Mpro of SARS-CoV-2 and H2B-mIFP
    Expression
    Mammalian
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    163079-plasmid-map-sequence-id-320826

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  12. pU6-ACTA2_gRNA1-SpCas9-T2A-GFP

    Plasmid
    #126706
    Purpose
    to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human ACTA2 locus at the end of the endogenous coding sequence
    Depositor
    Darrell Kotton
    Publication
    Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ACTA2_gRNA1-SpCas9-T2A-GF (ACTA2 Human)
    Use
    CRISPR
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    126706-plasmid-map-sequence-id-245754

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  14. pDEST-CMV 3xFLAG-LC3A-GFP

    Plasmid
    #123106
    Purpose
    Expresses 3xFLAG-LC3A-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3A
    Depositor
    Robin Ketteler
    Publication
    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)
    Tags
    3xFLAG and EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    123106-plasmid-map-sequence-id-244007

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  16. P3-dCas9-Tet1-GFP-Puro

    Plasmid
    #190728
    Purpose
    Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)
    Depositor
    Maja Jagodic
    Publication
    Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-tet1
    Use
    CRISPR
    Tags
    3XFLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    Changed Pro434 codon from CCG to CCC and Gly468 f…
    Promoter
    pCAG
    Available since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    190728-plasmid-map-sequence-id-381333

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  17. MSCV-opEBNA2-IRES-GFP

    Plasmid
    #220354
    Purpose
    Expresses EBV latent gene EBNA2 in mammalian cells (EBNA2 coding sequence was optimised for expression and carries a C-terminal 3xHA-tag)
    Depositor
    Klaus Rajewsky
    Publication
    Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d
    Insert
    opEBNA2 (EBNA-2 Epstein-Barr Virus (EBV) strain B95-8)
    Use
    Retroviral
    Tags
    3 x HA tag
    Expression
    Mammalian
    Mutation
    codon-optimised for expression in mammalian cells
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    220354-plasmid-map-sequence-id-437068

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Showing: 11041 - 11060 of 28117 results