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pCAGGS-RaichuEV-Rac1(T17N)

Plasmid

#164902

Purpose

Expresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation T17N in the Rac1 domain

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

RaichuEV-Rac1 (RAC1 Human)

Expression

Mammalian

Mutation

T17N mutation in the Rac1 domain which make the s…

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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KM-CashGem-Xyl2-URA3-LINEAR

Plasmid

#174838

Purpose

pRS414 backbone containing LINEAR fragment targeting XYL2 in K. marxianus

Depositor

Zengyi Shao

Article

Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

Insert

Codon optimized Cas9:hGem

Use

CRISPR and Synthetic Biology

Tags

hGeminin tag

Expression

Bacterial and Yeast

Promoter

TEF1p

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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LbCas12a-DR-cr in PBCSK-

Plasmid

#126641

Purpose

Cloning vector LbCas12a DR-crRNA for expression in mammalian cells

Depositor

Scot Wolfe

Article

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

LbCas12a Full Length Direct Repeat crRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6 Promoter

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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GST-PRDM16 1-223

Plasmid

#53346

Purpose

bacterial expression of mouse PRDM16 fragment

Depositor

Shingo Kajimura, Bruce Spiegelman

Article

Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

Insert

PRDM16 (Prdm16 Mouse)

Tags

GST

Expression

Bacterial

Mutation

aa 1-223

Promoter

tac

Available Since

June 2, 2014

Availability

Academic Institutions and Nonprofits only
pCALNL-Kir2.1

Plasmid

#125578

Purpose

Cre-dependent expression of Kir2.1 (FLAG-tagged) in mammalian cells.

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

Kir2.1 (Kcnj2 Mouse)

Use

Cre/Lox

Tags

FLAG

Expression

Mammalian

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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YIp128-CUP1-NUbo-GFP-His6

Plasmid

#212790

Purpose

Copper-inducible expression of the artificial test substrate NUbo-GFP-His6 in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

NUbo-GFP-His6

Use

Integrative vector

Expression

Yeast

Promoter

CUP1

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pHS1477

Plasmid

#205970

Purpose

MWEE01000013 Cas5-HNH operon (E. coli codon optimized) with His14-tagged Cse2 in pET45b(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas5-HNH all proteins, with His-tagged Cas11

Tags

14xHis on Cas11

Expression

Bacterial

Promoter

T7

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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GST-PRDM16 1039-1176

Plasmid

#53351

Purpose

bacterial expression of mouse PRDM16 fragment

Depositor

Shingo Kajimura, Bruce Spiegelman

Article

Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

Insert

PRDM16 (Prdm16 Mouse)

Tags

GST

Expression

Bacterial

Mutation

aa 1039-1176

Promoter

tac

Available Since

June 2, 2014

Availability

Academic Institutions and Nonprofits only
GST-PRDM16 681-880

Plasmid

#53349

Purpose

bacterial expression of mouse PRDM16 fragment

Depositor

Shingo Kajimura, Bruce Spiegelman

Article

Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

Insert

PRDM16 (Prdm16 Mouse)

Tags

GST

Expression

Bacterial

Mutation

aa 681-880

Promoter

tac

Available Since

June 2, 2014

Availability

Academic Institutions and Nonprofits only
GST-PRDM16 455-680

Plasmid

#53348

Purpose

bacterial expression of mouse PRDM16 fragment

Depositor

Shingo Kajimura, Bruce Spiegelman

Article

Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

Insert

PRDM16 (Prdm16 Mouse)

Tags

GST

Expression

Bacterial

Mutation

aa 455-680

Promoter

tac

Available Since

June 2, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-p97wt-mycStrep

Plasmid

#31837

Depositor

Hemmo Meyer

Article

Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

Insert

p97

Tags

Strep and myc

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only
pFUW-TetO-STAT2

Plasmid

#178449

Purpose

doxycycline-inducible expression of Human STAT2 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

STAT2 (STAT2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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bArr1-ALFA

Plasmid

#201496

Purpose

Expression of the beta-arrestin1 containing a C-terminal ALFA tag.

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

bArr1-ALFA (ARRB1 Human)

Tags

ALFA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pGEL636

Plasmid

#189830

Purpose

The T-DNA backbone of Cas12j2 for transcriptional activation in rice

Depositor

Yong Zhang

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Promoter

pZmUbi1

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGEL638

Plasmid

#189831

Purpose

The T-DNA backbone of nCas12j2 for genome editing in rice

Depositor

Yong Zhang

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR; T-dna clone

Promoter

ZmUbi1

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pUDE735

Plasmid

#103024

Purpose

expression of a Cpf1 programming crRNA targetting CAN1, HIS4, PDR12 and ADE2 (crCAN1-4.crHIS4-4.crPDR12-3.crADE2-3.S)

Depositor

Jean-Marc Daran

Article

Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

Insert

crCAN1-4.crHIS4-4.crPDR12-3.crADE2-3.S

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Xpress-GBF1

Plasmid

#206417

Purpose

Mammalian expression of hGBF1

Depositor

Kazuhisa Nakayama

Article

Kawamoto et al Traffic. 2002 Jul;3(7):483-95. doi: 10.1

Insert

GBF1 (GBF1 Human)

Expression

Mammalian

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold-Actin-C-18

Plasmid

#158009

Purpose

Visualization of actin in mammalian cells using mGold

Depositor

Francois St-Pierre

Article

Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

Insert

Actin (ACTB Human)

Tags

mGold

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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1129 pcDNA3 GFP FKHR H215R AAA

Plasmid

#9034

Depositor

William Sellers

Article

Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

Insert

FKHR H215R AAA (FOXO1 Human)

Tags

GFP

Expression

Mammalian

Mutation

H215R T24A S256A S319A

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pDECKO_mCherry_CD19.4

Plasmid

#140101

Purpose

CRISPR-Cas9 Knock down

Depositor

Roderic Guigo

Article

Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Available Since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

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