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aTY135_mtk02_pCAG-nfGFPtether_P2A_H2B-IRFP_SV40pA

Plasmid

#225350

Purpose

piggyback backbone for non-fluorescent GFP tether and H2B-IRFP expression

Depositor

Wendell Lim

Article

Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

Insert

H2B (H2B Human, Synthetic)

Tags

miRFP and non-fluorescent GFP (Y67F)-P2A

Expression

Mammalian

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pIRGAL4A

Plasmid

#218277

Purpose

Introduction of a LowTempGAL Turbo module (GAL4) to accelerate induction of GAL promoter induction

Depositor

Claudia Vickers

Article

Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

Insert

fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-fcy1(454-778)

Expression

Yeast

Available Since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pUBQ10:LYK5_At-3xHA

Plasmid

#202192

Purpose

Express Arabidopsis thaliana LYK5 gene under UBQ10 promoter

Depositor

Gitta Coaker

Article

Trinh et al Plant Physiol. 2023 May 5:kiad263. doi:

Insert

LYSM-CONTAINING RECEPTOR-LIKE KINASE 5 (LYK5 Mustard Weed)

Tags

HA

Expression

Plant

Promoter

AtUBQ10

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAcGFP1-N1-RvCAHS12-min-GS

Plasmid

#192465

Purpose

Expression of RvCAHS12-min-GS_linker-AcGFP1 in mammalian cells

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

Insert

CAHS12-min

Tags

AcGFP1

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAcGFP1-N1-RvCAHS3-min-GS

Plasmid

#192463

Purpose

Expression of RvCAHS3-min-GS_linker-AcGFP1 in mammalian cells

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

Insert

CAHS3-min

Tags

AcGFP1

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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phABCA3-eGFP_CRISPR_Donor

Plasmid

#188540

Purpose

Homologous recombination donor plasmid for CRISPR/Cas9 targeting of GFP fusion protein to the stop codon of endogenous human ABCA3 gene locus

Depositor

Darrell Kotton

Article

Sun et al Am J Respir Cell Mol Biol. 2021 Oct;65(4

Insert

EGFP

Use

CRISPR; Donor for homologous recombination

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCDF1-ADAMTS18 (P1035S)

Plasmid

#29558

Depositor

Yardena Samuels

Article

Wei et al Mol Cancer Res. 2010 Nov . 8(11):1513-25

Insert

ADAMTS18 (ADAMTS18 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

changed proline 1035 to serine

Available Since

Sept. 7, 2011

Availability

Academic Institutions and Nonprofits only
pCDF1-ADAMTS18 (C638S)

Plasmid

#29555

Depositor

Yardena Samuels

Article

Wei et al Mol Cancer Res. 2010 Nov . 8(11):1513-25

Insert

ADAMTS18 (ADAMTS18 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

changed cysteine 638 to serine

Available Since

July 22, 2011

Availability

Academic Institutions and Nonprofits only
pICSL50026

Plasmid

#174590

Purpose

Level 0 C-terminal tag module carrying 3C-6xHIS

Depositor

Mark Banfield

Insert

3C-6xHIS

Use

Synthetic Biology

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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Sc Rad27-S6- pET24

Plasmid

#239205

Purpose

Overexpress Sc Rad27 (FEN1) (C-terminal S6 tag) in E. coli

Depositor

Michael O'Donnell

Insert

Rad27 (RAD27 Budding Yeast)

Tags

S6

Expression

Bacterial

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pICSL50025

Plasmid

#174589

Purpose

Level 0 C-terminal tag module carrying 6xHIS uncleavable

Depositor

Mark Banfield

Insert

6xHIS uncleavable

Use

Synthetic Biology

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pmCherry-Gal3

Plasmid

#85662

Purpose

Fluorescent reporter for lysosomal damage

Depositor

Hemmo Meyer

Article

Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Insert

LGALS3 (LGALS3 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pmCherry N1 MFN2

Plasmid

#157759

Purpose

Expression of MFN2:mcherry in cells

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

MFN2 (MFN2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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ch-TOGKDP-GFP

Plasmid

#69112

Purpose

For mammalian expression of knockdown-proof human ch-TOG tagged with EGFP.

Depositor

Stephen Royle

Article

Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

Insert

Colonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Silent mutations to confer shRNA resistance.

Promoter

CMV

Available Since

Oct. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pmCherry C1 Omp25

Plasmid

#157758

Purpose

Expression of mcherry-labelled mitochondrial outer membrane protein 25 in cells

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

SYNJ2bp (SYNJ2BP Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-hMC4R

Plasmid

#179315

Purpose

Express human melanocortin 4 receptor (GFP tagged) in mammalian cells

Depositor

Christian Gruber

Article

Muratspahic et al ACS Pharmacol Transl Sci. 2023 Sep 26;6(

Insert

melanocortin 4 receptor (MC4R Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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peGFP C3-SPG20 8xV

Plasmid

#218579

Purpose

Expresses GFP-tagged human SPG20 bulky hydrophobic amino acid mutant in mammalian cells

Depositor

Hemmo Meyer

Article

Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

Insert

SPG20 (SPART Human)

Tags

eGFP

Expression

Mammalian

Mutation

changed Tryptophan 419, Leucine 438, Isoleucine 4…

Promoter

CMV

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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UbC-blasticidine-P2A-TETon-3G transactivator

Plasmid

#200191

Purpose

A TETon activator, which combined with the DiLiCre 2.0 plasmid, drives the expression of DiLiCre 2.0 upon doxycycline. DiLiCre 2.0 is a cre recombinase that is activated by violet light.

Depositor

Jacco van Rheenen

Article

Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:

Insert

blasticidine - P2A - TETon-3G transactivator

Use

Lentiviral

Expression

Bacterial and Mammalian

Promoter

UbC

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-ORP8-H514A-H515A

Plasmid

#208360

Purpose

Mammalian expression of fluorescent N-terminally tagged H514A-H515A mutant of ORP8

Depositor

Paula Nunes-Hasler

Article

Criado Santos et al Commun Biol. 2023 Oct 4;6(1):1008. doi:

Insert

ORP8 (OSBPL8 Human)

Tags

EGFP

Expression

Mammalian

Mutation

H514A-H515A

Promoter

CMV

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pMYH9-D1424H-FLAG

Plasmid

#183513

Purpose

Gateway expression vector encoding D1424H mutant MYH9 cDNA, with N-terminal Flag tag. For mammalian expression.

Depositor

Emanuele Panza

Article

Panza et al Pathogenetics. 2008 Dec 1;1(1):5. doi: 1

Insert

MYH9 (MYH9 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Aspartic acid 1424 to Histidine

Promoter

CMV

Available Since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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