Addgene

We narrowed to 9,769 results for: otos

Showing: 1651 - 1680 of 9769 results

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LEPG_shSik3.3974

Plasmid

#125855

Purpose

Retroviral expression of mouse Sik3 shRNA with GFP and puromycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

Insert

shRNA targeting Sik3

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

MSCV-LTR

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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LEPG_shSik3.308

Plasmid

#125878

Purpose

Retroviral expression of mouse Sik3 shRNA with GFP and puromycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

Insert

shRNA targeting Sik3

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

MSCV-LTR

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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VCDstub-GFP

Plasmid

#33122

Depositor

Greg Phillips

Article

O'Leary et al Mol Biol Cell. 2011 Sep 14.

Insert

Pcdh-γA3 (Pcdhga3 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Contains a signal sequence fused to a very small …

Promoter

CMV

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
ΔCT190-GFP

Plasmid

#33118

Depositor

Greg Phillips

Article

O'Leary et al Mol Biol Cell. 2011 Sep 14.

Insert

Pcdh-γA3 (Pcdhga3 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of the last 190 aa of the variable cytop…

Promoter

CMV

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
SRGAP2C_pEF-DEST51

Plasmid

#238497

Purpose

IVT mRNA of human gene

Depositor

Megan Dennis

Article

Soto et al bioRxiv [Preprint]. 2024 Sep 26:2024.09.

Insert

SRGAP2C (SRGAP2C Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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PDZK1P1_pGCS1

Plasmid

#238501

Purpose

IVT mRNA of human gene

Depositor

Megan Dennis

Article

Soto et al bioRxiv [Preprint]. 2024 Sep 26:2024.09.

Insert

PDZK1P1 (PDZK1P1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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ARHGAP11B_pEF-DEST51

Plasmid

#238498

Purpose

IVT mRNA of human gene

Depositor

Megan Dennis

Article

Soto et al bioRxiv [Preprint]. 2024 Sep 26:2024.09.

Insert

ARHGAP11B (ARHGAP11B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PM-deltaCKAR

Plasmid

#222429

Purpose

FRET-based reporter for monitoring deltaPKC activity at the plasma membrane in cells.

Depositor

Alexandra Newton

Article

Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

Insert

Plasma membrane delta-selective C kinase activity reporter

Use

Tags

CFP and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Nuc-deltaCKAR

Plasmid

#222430

Purpose

FRET-based reporter for monitoring deltaPKC activity at the nucleus in cells.

Depositor

Alexandra Newton

Article

Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

Insert

Nucleus delta-selective C kinase activity reporter

Use

Tags

CFP and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Golgi-deltaCKAR

Plasmid

#222428

Purpose

FRET-based reporter for monitoring deltaPKC activity at the Golgi in cells.

Depositor

Alexandra Newton

Article

Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

Insert

Golgi delta-selective C kinase activity reporter

Use

Tags

CFP and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221_zCas9

Plasmid

#213913

Purpose

Entry clone containing 3×FLAG-NLS-zCas9-NLS encoding sequence, Pisum sativum rbcS E9 terminator. For use in plants and compatible with the MultiSite Gateway system

Depositor

Sébastien Mongrand

Article

Li et al Bio Protoc. 2024 Jul 5;14(13):e5029. doi

Insert

zCas9

Use

CRISPR

Tags

Expression

Mutation

Zea mays codon-optimized Cas9

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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AAV hSyn mRuby

Plasmid

#220912

Purpose

Neuronal specific expression of mRuby

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Insert

mRuby

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pJH2673

Plasmid

#199691

Purpose

Pnmr-1 FLPe unc-54 3' UTR C.elegans AVA/E/D premotor IN and others expression of FLPe

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

flpE (flpE )

Use

Tags

no

Expression

Worm

Mutation

Promoter

Pnmr-1 long

Available since

May 7, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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Ph-NylC_pET28a

Plasmid

#215425

Purpose

Expression construct for Ph-NylC gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

Ph-NylC

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pDN0520 (pDEST, TRP1)

Plasmid

#210481

Purpose

pDEST vector with TRP1 marker for gene expression in S. cerevisiae.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Destination vector for gateway cloning

Tags

Expression

Mutation

Promoter

ADH1

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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Vd-ARATR-GFP

Plasmid

#208903

Purpose

encodes the arachnotocin receptor (oxytocin/vasopressin-like) from the mite Varroa destructor, tagged with GFP

Depositor

Christian Gruber

Article

Duerrauer et al Sci Rep. 2019 Dec 17;9(1):19295. doi: 10

Insert

ARATR

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pTarget_PS_VA

Plasmid

#186454

Purpose

pTarget containing protospacer for FnCas12a targeting

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTarget_PS_VB

Plasmid

#186456

Purpose

pTarget containing protospacer for AaCas12b targeting

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSub-Asp6-MBP-HRas

Plasmid

#185758

Purpose

Bacterial expression of Asp6-MBP-HRas

Depositor

Jeffrey Bode

Article

Akimoto et al ACS Cent Sci. 2022 Feb 23;8(2):275-281.

Insert

Asp6-MBP-HRas

Use

Tags

MBP

Expression

Bacterial

Mutation

6x Asp added to the N terminus of MBP

Promoter

tac

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pPB-tetpA(R1R4)-iRPT

Plasmid

#154882

Purpose

Ubiquitously expresses iRFP713, Puromycin resistance gene, and rtTAM2.

Depositor

Hideyuki Okano

Article

Nemoto et al Cells. 2020 Dec 22;10(1). pii: cells1001

Inserts

iRFP713
Puromycin resistance gene
rtTAM2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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PCDHGC5 sh1 3m shRNA

Plasmid

#122228

Purpose

Negative control shRNA (with 3-point mutations), for plasmid 122227: targeting Protocadherin gamma C5 mRNA.

Depositor

Angel de Blas

Article

Li et al J Neurosci. 2012 Aug 22;32(34):11780-97.

Insert

PCDHGC5 shRNA (Pcdhgc5 Rat)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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ΔCT163-GFP

Plasmid

#33116

Depositor

Greg Phillips

Article

O'Leary et al Mol Biol Cell. 2011 Sep 14.

Insert

Pcdh-γA3 (Pcdhga3 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of the last 163 aa of the variable cytop…

Promoter

CMV

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
ΔCT183-GFP

Plasmid

#33117

Depositor

Greg Phillips

Article

O'Leary et al Mol Biol Cell. 2011 Sep 14.

Insert

Pcdh-γA3 (Pcdhga3 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of the last 183 aa of the variable cytop…

Promoter

CMV

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
VCDstubΔ741-752-GFP

Plasmid

#33123

Depositor

Greg Phillips

Article

O'Leary et al Mol Biol Cell. 2011 Sep 14.

Insert

Pcdh-γA3 (Pcdhga3 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Contains a signal sequence fused to a very small …

Promoter

CMV

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
plIG-123_HA-GD2-28z_CAR_TF_Library

Pooled Library

#207478

Purpose

HA-GD2-28z CAR in combination with 100 transcription factors (and related proteins) and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into th

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Species

Homo sapiens

Use

CRISPR

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only
plIG-128_HA-GD2-28z_CAR_TFxTF_Library

Pooled Library

#207480

Purpose

HA-GD2-28z CAR in combination with ~10,000 transcription factor combinations (and related proteins) and controls (GFP/RFP combinations). Library can be used to generate HDR templates for modular poole

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Use

CRISPR

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only
plIG-106_NY-ESO-1_TCR_SR_Library

Pooled Library

#207474

Purpose

NY-ESO-1 TCR in combination with 129 surface receptors and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into the TRAC locus of human T cells

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Species

Homo sapiens

Use

CRISPR

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only
plIG-107_NY-ESO-1_TCR_TF_Library

Pooled Library

#207475

Purpose

NY-ESO-1 TCR in combination with 100 transcription factors (and related proteins) and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into the

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Species

Homo sapiens

Use

CRISPR

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only
plIG-109_CD19-BBz_CAR_SR_Library

Pooled Library

#207476

Purpose

CD19-BBz CAR in combination with 129 surface receptors and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into the TRAC locus of human T cells

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Use

CRISPR

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only
plIG-110_CD19-BBz_CAR_TF_Library

Pooled Library

#207477

Purpose

CD19-BBz CAR in combination with 100 transcription factors (and related proteins) and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into the

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Species

Homo sapiens

Use

CRISPR

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only