We narrowed to 1,670 results for: nif

1. pPP378

   Plasmid

   #158797

   Purpose

   pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-1(D371A) in MCS1 for expression in E. coli and protein purification

   Depositor

   Jennifer Doudna

   Article

   Pausch et al Science. 2020 Jul 17;369(6501):333-337.

   Insert

   dCasPhi-1 (D371A)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. p2CT-His-MBP-Rca_Cas13a_WT

   Plasmid

   #91870

   Purpose

   Bacterial expression for Cas13a

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

   Insert

   RcaCas13a (RcaC2c2) WT
   

   Use

   Tags

   His6-MBP-TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCF525-EF1a-Hygro-P2A-AcrVA1-lenti

   Plasmid

   #115662

   Purpose

   Expresses AcrVA1 in human cells, contained in a lentiviral vector.

   Depositor

   Jennifer Doudna

   Article

   Watters et al Science. 2018 Oct 12;362(6411):236-239.

   Insert

   AcrVA1
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a promoter

   Available Since

   Oct. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSHS293

   Plasmid

   #87348

   Purpose

   D435C/E945C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, lobe closure FRET construct

   Depositor

   Jennifer Doudna

   Article

   Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

   Insert

   Cas9
   

   Use

   Tags

   His-MBP

   Expression

   Bacterial

   Mutation

   C80S/D435C/C574S/E945C

   Promoter

   T7

   Available Since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPP384

   Plasmid

   #158799

   Purpose

   pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-3(D413A) in MCS1 for expression in E. coli and protein purification

   Depositor

   Jennifer Doudna

   Article

   Pausch et al Science. 2020 Jul 17;369(6501):333-337.

   Insert

   dCasPhi-3 (D413A)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. T1P/pUBQ10(mTurq->mScarlet)

   Plasmid

   #195945

   Purpose

   PhiC31 target with pUBQ10 switching from mTurq to mScarlet

   Depositor

   Jennifer Nemhauser

   Article

   Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

   Insert

   tRBCS-mScarlet-attP_PhiC31-pUBQ10-attB_PhiC31-mTurq-tUBQ10
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. p2CT-His-MBP-Cam_Cas13a_WT

   Plasmid

   #91869

   Purpose

   Bacterial expression for Cas13a

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

   Insert

   CamCas13a (CamC2c2) WT
   

   Use

   Tags

   His6-MBP-TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. p2CT-His-MBP-Ere_Cas13a_WT

   Plasmid

   #91868

   Purpose

   Bacterial expression for Cas13a

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

   Insert

   EreCas13a (EreC2c2) WT
   

   Use

   Tags

   His6-MBP-TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSHS248

   Plasmid

   #87354

   Purpose

   S867C/N1054C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-2 FRET construct

   Depositor

   Jennifer Doudna

   Article

   Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

   Insert

   Cas9
   

   Use

   Tags

   His-MBP

   Expression

   Bacterial

   Mutation

   C80S/C574S/S867C/N1054C

   Promoter

   T7

   Available Since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. human EB1-GFP mutagenized S16 R17 H18 D19 W23 N25 all converted to A (JB139)

    Plasmid

    #39300

    Depositor

    Tim Mitchison, Jennifer Tirnauer

    Insert

    EB1 (MAPRE1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S16A, R17A, H18A, D19A, W23A and N25A

    Promoter

    CMV

    Available Since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pET_His6-MBP-TEV-Cas2

    Plasmid

    #186275

    Purpose

    Expresses Thiomicrospira Cas2 in E. coli

    Depositor

    Jennifer Doudna

    Article

    Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

    Insert

    Thiomicropira Cas2
    

    Use

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGP4G-TPLRD1-ccdB

    Plasmid

    #83877

    Purpose

    Destination vector for integration at yeast Trp locus with N-terminal TPL repression domain (RD) for auxin response assays

    Depositor

    Jennifer Nemhauser

    Article

    Pierre-Jerome et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. L0-I39 PhiC31-Intein GG1-GG4

    Plasmid

    #195905

    Purpose

    Level 0 PhiC31-intein(DnaE-Npu) with GG1 and GG4 golden gate linkers

    Depositor

    Jennifer Nemhauser

    Article

    Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

    Insert

    PhiC31-intein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. L0-I40 Intein-PhiC31 GG1-GG4

    Plasmid

    #195906

    Purpose

    Level 0 Intein(DnaE-Ssp)-PhiC31 with GG1 and GG4 golden gate linkers

    Depositor

    Jennifer Nemhauser

    Article

    Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

    Insert

    Intein-PhiC31
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. 7xNLS-pMJ915v2-sfGFP

    Plasmid

    #88922

    Purpose

    For expression of modified SpyCas9 in e.coli.

    Depositor

    Jennifer Doudna

    Article

    Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Inserts

    Cas9
    T7
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-3a-His-MBP-TEV-VC_Cas1_WT

    Plasmid

    #120364

    Purpose

    Expresses wildtype V-C Cas1

    Depositor

    Jennifer Doudna

    Article

    Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

    Insert

    VC_Cas1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET-3a-His-MBP-TEV-VD_Cas1_WT

    Plasmid

    #120365

    Purpose

    Expresses wildtype V-D Cas1

    Depositor

    Jennifer Doudna

    Article

    Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

    Insert

    VD_Cas1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pVC-CRISPR1

    Plasmid

    #120366

    Purpose

    Plasmid containing shortened version of upstream V-C CRISPR array for integration assays

    Depositor

    Jennifer Doudna

    Article

    Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

    Insert

    CRISPR1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pVC-CRISPR

    Plasmid

    #120369

    Purpose

    Plasmid containing shortened versions of both natural V-C CRISPR arrays for preparing sequencing library

    Depositor

    Jennifer Doudna

    Article

    Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

    Inserts

    CRISPR1
    CRISPR2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pVD_CRISPR

    Plasmid

    #120370

    Purpose

    Plasmid containing shortened version of V-D CRISPR array for integration assays

    Depositor

    Jennifer Doudna

    Article

    Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

    Insert

    VD_CRISPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge